Additional file 3: of Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

Figure S2. A longitudinal study on a single GBM patient was carried out in order to monitor the changes in serum HOTAIR expression over time. 3 different time points were included in this study: pre-op (the blood was drawn right before the surgery started), post-op (at least 24 h after surgery) and during the 2 week follow-up (F/U) with the neurosurgeon. We show that the level of HOTAIR decreases after surgery and at the follow-up visit. (PDF 430 kb

Additional file 1: of Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

Supplementary materials and methods. (DOCX 109 kb

Additional file 2: of Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

Figure S1. HOTAIR expression detected in our biomarker assay is derived mostly from circulating RNA, not DNA. 3 GBM serum samples were selected at random and the relative HOTAIR expression in GBM serum with and without reverse transcription (RT)-PCR was determined. The HOTAIR RNA was reverse-transcribed into HOTAIR cDNA and qPCR was performed. The circulating HOTAIR DNA in the serum was detected by qPCR without RT. The considerable difference between HOTAIR expression with and without RT demonstrates that the HOTAIR we are detecting in our qRT-PCR reactions is derived from RNA and not DNA. (PDF 1003 kb

Additional file 2: of Prenatal arsenic exposure alters the placental expression of multiple epigenetic regulators in a sex-dependent manner

Associations of candidate gene expression in fetal placenta with maternal U-As. Multivariable linear regression analysis was performed to determine the association between log-transformed maternal U-As and log-transformed fetal placental expression of epigenetic candidate genes, in A) the unstratified cohort, B) males, and C) females. Analyses were adjusted for maternal age at enrollment. P values before and after FDR adjustment are shown (columns C-D). The effect of adjustment for urinary creatinine was also determined, by comparing the standardized beta values obtained from the subset of participants for which urinary creatinine data were available (“standardized_subset_beta”, column P) with the equivalent values after adjustment for urinary creatinine (“standardized_subset_beta”, column Q). Results of goodness-of-fit tests are also shown (columns S-T). (XLSX 124 kb

Additional file 3: of Prenatal arsenic exposure alters the placental expression of multiple epigenetic regulators in a sex-dependent manner

Associations of fetal placental candidate gene expression with maternal U-As. Graphical representation of associations between maternal U-As and fetal placental expression of epigenetic candidate genes in the unstratified cohort. Analysis was adjusted for maternal age at enrollment. Dots depict coefficient estimates and error bars represent 95% CIs. Significant associations are those with 95% CIs not crossing zero (red dotted line). (PDF 133 kb

Additional file 1: of Prenatal arsenic exposure alters the placental expression of multiple epigenetic regulators in a sex-dependent manner

Candidate genes used in this study (XLSX 60 kb

Additional file 5: of Prenatal arsenic exposure alters the placental expression of multiple epigenetic regulators in a sex-dependent manner

Associations of candidate gene expression in male fetal placenta with maternal U-As. Graphical representation of associations between maternal U-As and expression of epigenetic candidate genes in male fetal placentas. Analysis was adjusted for maternal age at enrollment. Dots depict coefficient estimates and error bars represent 95% CIs. Significant associations are those with 95% CIs not crossing zero (red dotted line). (PDF 102 kb