Synthesis of Anisotropic Magnetic Polymeric Janus Particles by In Situ Separation of Magnetic Nanoparticles in a Microfluidic Device

Magnetic Janus particles have been studied extensively for medical and biological applications owing to their controllable mobility in fluid media. In this work, we report a novel microfluidic device designed for the synthesis of magnetically anisotropic Janus particles made of poly(ethylene glycol) diacrylate and embedded with magnetic iron oxide nanoparticles. Our method consists of a droplet generation step followed by magnetic separation using an external magnetic field and ultraviolet polymerization. The synthesized particles exhibit a monodisperse size distribution with a standard deviation of less than 3.5%, which is among the best size distributions obtained in the literature for magnetic Janus particles. The anisotropic magnetic property of the particles enable them to rotate about their own axes in the presence of an external magnetic field, introducing another degree of freedom to their motion. This microfluidic technique is simple, one-step, and versatile, offering control over the size distribution to synthesize magnetically anisotropic Janus particles

Synthesis of Anisotropic Magnetic Polymeric Janus Particles by In Situ Separation of Magnetic Nanoparticles in a Microfluidic Device

Magnetic Janus particles have been studied extensively for medical and biological applications owing to their controllable mobility in fluid media. In this work, we report a novel microfluidic device designed for the synthesis of magnetically anisotropic Janus particles made of poly(ethylene glycol) diacrylate and embedded with magnetic iron oxide nanoparticles. Our method consists of a droplet generation step followed by magnetic separation using an external magnetic field and ultraviolet polymerization. The synthesized particles exhibit a monodisperse size distribution with a standard deviation of less than 3.5%, which is among the best size distributions obtained in the literature for magnetic Janus particles. The anisotropic magnetic property of the particles enable them to rotate about their own axes in the presence of an external magnetic field, introducing another degree of freedom to their motion. This microfluidic technique is simple, one-step, and versatile, offering control over the size distribution to synthesize magnetically anisotropic Janus particles

Synthesis of Anisotropic Magnetic Polymeric Janus Particles by In Situ Separation of Magnetic Nanoparticles in a Microfluidic Device

Magnetic Janus particles have been studied extensively for medical and biological applications owing to their controllable mobility in fluid media. In this work, we report a novel microfluidic device designed for the synthesis of magnetically anisotropic Janus particles made of poly(ethylene glycol) diacrylate and embedded with magnetic iron oxide nanoparticles. Our method consists of a droplet generation step followed by magnetic separation using an external magnetic field and ultraviolet polymerization. The synthesized particles exhibit a monodisperse size distribution with a standard deviation of less than 3.5%, which is among the best size distributions obtained in the literature for magnetic Janus particles. The anisotropic magnetic property of the particles enable them to rotate about their own axes in the presence of an external magnetic field, introducing another degree of freedom to their motion. This microfluidic technique is simple, one-step, and versatile, offering control over the size distribution to synthesize magnetically anisotropic Janus particles

Massive attack by honeybees in a German shepherd dog: description of a fatal case and review of the literature

In the present study, a fatal case caused by honeybee (Apis cerana) stings was documented in a female German shepherd dog that was presented at the Veterinary Teaching Hospital, University of Agriculture Faisalabad, Pakistan. Characteristic clinical signs included hematuria, hematemesis, incoordination and convulsions along with evidence of massive honeybee attack supported the diagnosis of envenomation. The dog was treated with dexamethasone and diphenhydramine, but it did not respond to therapy and died. This outcome could be avoided if we had a bee antivenom available for treating envenomated patients.</div

Lucigenin-Tris(2-carboxyethyl)phosphine Chemiluminescence for Selective and Sensitive Detection of TCEP, Superoxide Dismutase, Mercury(II), and Dopamine

Development of simple and effective chemiluminescence (CL) systems for multiple sensing applications is significantly important but still a challenge. Until now, the majority of CL systems primarily utilized hydrogen peroxide (H2O2) as coreactant, which is limited in its stability and selectivity due to the easy decomposition of H2O2 in the presence of several ions. In this study, we develop a new and intense CL system by combined use of tris­(2-carboxyethyl)­phosphine (TCEP), a highly solution stable and pH-tolerant tertiary phosphine, with lucigenin for the first time. The effective pairing leads to a significant ∼23 times CL enhancement over classic the lucigenin–H2O2 system without employing additional catalysts. By virtue of this fascinating platform, a sensitive CL method has been developed for the multiple detection of TCEP (LOD = 70 nM), lucigenin (LOD = 4.0 nM), superoxide dismutase (LOD = 0.8 ng/mL), Hg2+(LOD = 0.3 nM), and dopamine (LOD = 3.0 nM), with a linear range of 0.1–320 μM, 0.01–55 μM, 0.005–0.5 μg/mL, 1.0–600 nM, and 0.01–0.8 μM, respectively. Remarkably, this CL method exhibited superior selectivity over several potential interferents. Moreover, the proposed method achieved excellent recoveries in the range of 94.0–102.3% for both Hg2+ detection in lake water and dopamine detection in human serum real samples. We envision that broad applications of TCEP may lead to construct new CL systems, pushing forward for efficient detection of various analytes

Maximum likelihood phylogenetic tree of amino acid sequences of coat proteins of isolates of the carlaviruses garlic common latent virus (GCLV) (red dots) and shallot latent virus (SLV) (black dots).

<p>Shown for each isolate is GenBank accession code, isolate name and country of origin. Isolates described in this study are indicated by a dot.</p

Maximum likelihood phylogenetic tree of amino acid sequences of replicase proteins of isolates of potexviruses.

<p>Shown for each isolate is GenBank accession code, and virus name. The sequence representing the new Asparagus virus 3 (AV3) isolate is indicated by a black dot. The homologous region of an isolate of garlic virus A (<i>Allexivirus</i>) was used as the outgroup.</p

Zirconium-Directed Supramolecular Self-Assembly of Coenzyme A@GNCs with Enhanced Phosphorescence for Developing Ultrasensitive Tracer Probe of Dipicolinic Acid, a Biomarker of Bacterial Spores

Luminescent gold nanoclusters (GNCs) are a class of attractive quantum-sized nanomaterials bridging the gap between organogold complexes and gold nanocrystals. They typically have a core–shell structure consisting of a Au(I)-organoligand shell-encapsulated few-atom Au(0) core. Their luminescent properties are greatly affected by their Au(I)-organoligand shell, which also supports the aggregation-induced emission (AIE) effect. However, so far, the luminescent Au nanoclusters encapsulated with the organoligands containing phosphoryl moiety have rarely been reported, not to mention their AIE. In this study, coenzyme A (CoA), an adenosine diphosphate (ADP) analogue that is composed of a bulky 5-phosphoribonucleotide adenosine moiety connected to a long branch of vitamin B5 (pantetheine) via a diphosphate ester linkage and ubiquitous in all living organisms, has been used to synthesize phosphorescent GNCs for the first time. Interestingly, the synthesized phosphorescent CoA@GNCs could be further induced to generate AIE via the PO32– and Zr4+ interactions, and the observed AIE was found to be highly specific to Zr4+ ions. In addition, the enhanced phosphorescent emission could be quickly turned down by dipicolinic acid (DPA), a universal and specific component and also a biomarker of bacterial spores. Therefore, a Zr4+-CoA@GNCs-based DPA biosensor for quick, facile, and highly sensitive detection of possible spore contamination has been developed, showing a linear concentration range from 0.5 to 20 μM with a limit of detection of 10 nM. This study has demonstrated a promising future for various organic molecules containing phosphoryl moiety for the preparation of AIE-active metal nanoclusters

Viruses detected from garlic (<i>Allium sativum</i>) and wild garlic (<i>A. vineale</i>) plants.

a<p>Country or area where plant was sourced.</p>b<p>Source: Allium vineale.</p>c<p>AV3, Asparagus virus 3; GCLV, garlic common latent virus; SLV, shallot latent virus; OYDV, onion yellow dwarf virus; LYSV, leek yellow stripe virus; GarVA, garlic virus A; GarVB, garlic virus B; GarVC, garlic virus C; GarVD, garlic virus D; GarVX, garlic virus X. Isolate names, GenBank accession codes, and nucleotide sequence lengths (in parenthesises) are shown. Complete or near-complete genome sequences indicated by an asterisk.</p>d<p>Commercial garlic grower.</p>e<p>Commercial plant nursery.</p>f<p>Kitchen garden.</p