4 research outputs found

    Effects of Folic Acid and Magnesium Co-Administration on Blood Glucose Levels in Streptozotocin-Induced Type I Diabetic Wistar Rats

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    Abstract: This work was designed to study the effects of FA+ Mg (folic acid and magnesium) co-administration on blood glucose levels of STZ (streptozotocin) induced diabetic rats. Healthy albino rats weighing between 150 g and 200 g were used. The rats were randomly allotted into six groups, each containing five albino rats respectively. Five of the groups ( II, III, IV, V and VI) were induced with diabetes by the by i.p. (intraperitoneal) injection of freshly prepared in 0.1 mol/L citrate buffered solution (pH 4.5) of streptozotocin (Sigma Aldrich, St. Louis, MO, USA) at a dose of 60 mg/kg body weight. Control (vehicle) rats were injected with equal volume of 0.1 mol/L citrate buffer. Four days after STZ injection, diabetes induction was confirmed by measuring fasting blood glucose level in a tail vein blood samples using ACCU-CHEK compact plus glucometer (Roche, France). Rats with glucose level of 200 mg/dl or higher were considered as diabetic. After the induction of diabetes, the rats were treated using the folic acid and magnesium separately and in combination respectively according to group daily, whereas, the other group (a) was not given any treatment and this served as the normal control, providing a baseline data. Blood samples were collected from the rat tail vein weekly for a period of four weeks. Results obtained from the study showed that FA+ Mg administered conjointly lowered blood glucose levels after 4 weeks of treatment when compared to diabetic control, significantly (P < 0.05). The action of the co-administration of folic acid and magnesium on blood glucose in diabetic rats was similar to that of Insulin (6 IU/mL), a potent hypoglycaemic agent. Oral supplementation of folic acid (20 mg/kg b.w. /day) and MgCl 2 (500/kg b.w. /day) separately or in combination for 4 weeks of treatment exhibited differential protective response in lowering the blood glucose levels following treatments. It was also found that during combined exposure of folic acid and magnesium, the adverse effects of the diabetes induced by STZ were less pronounced in the group that had FA+ Mg than their individual effects. This suggests the synergistic beneficial effects of folic acid and Magnesium against STZ-induced diabetes in Wistar Rats

    Antidiarrhoeal effect of the crude methanol extract of the dried fruit of Adansonia digitata L. (Malvaceae)

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    Aim: The study was designed to evaluate the antidiarrhoeal property of the methanol extract of the fruit of Adansonia digitata using laboratory animal models. Materials and Methods: The acute oral toxicity (LD50) of the extract was determined in mice, while the antidiarrhoeal effect of different doses of the extract was evaluated in both mice and rats. The effect of the extract at doses of 300, 700 and 1000 mg/kg were tested against intestinal transit time, magnesium sulphate-induced gastrointestinal motility test and castor oil-induced diarrhoea, respectively in mice. Similarly, the extract at the doses mentioned above was evaluated against castor-oil induced enteropooling in rats. Results: The methanol extract of A. digitata was shown to have no toxic effect in mice at doses up to 5000 mg/kg. At doses of 300 and 700 mg/kg the extract significantly (p<0.01) decrease intestinal transit time in mice. In addition, the extract at doses of 300, 700 and 1000 mg/kg, significantly (p<0.05) lowered diarrhoea induced by magnesium sulphate and castor oil in mice. Similarly, the extract produced a significant (p<0.05) inhibition of enteropooling caused by castor oil in rats. The antidiarrhoeal effect of the extract was shown to be dose-dependent. Conclusion: These findings revealed the potential of the extract of fruit of A. digitata as an antidiarrhoeal agent. The detailed mechanism of the extract antidiarrhoeal action is yet to be ascertained. However, one or more of the phytoconstituents contained in the extract could be responsible for the observed antidiarrhoeal effect

    Role of maternal variables on the development of neonatal hypoglycaemia and influence of neonatal hypoglycaemia on performance of goat kids

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    The study evaluated the influence of maternal variables (age, body weight and body mass index; BMI) during mating on the development of hypoglycaemia and investigated whether hypoglycaemia at birth impairs thermoregulation, metabolism, body weight gain and immunoglobulin concentration in neonatal goat kids. Post-kidding, the kids born with hypoglycaemia (n = 19) and normoglycaemia (n = 19) were immediately identified and postnatal blood samples, body weight and cardinal physiological variables were determined. Results revealed no significant (P 0.05) effect in hypoglycaemic kids. A more pronounced decrease (P < 0.05) in weekly weight gain was observed in hypoglycaemic kids. It was concluded that neonatal goat kids born with hypoglycaemia may have compromised thermoregulation, metabolism and body weight gain, and the cause of hypoglycaemia in kids may not be related to pre-mating maternal variables

    Available at www.veterinaryworld.org/Vol.7/July-2014/10.pdf RESEARCH ARTICLE Open Access Antidiarrhoeal effect of the crude methanol extract of the dried fruit of

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    the crude methanol extract of the dried fruit of Adansonia digitata L. (Malvaceae), Veterinary World 7(7): 495-500. Aim: The study was designed to evaluate the antidiarrhoeal property of the methanol extract of the fruit of Adansonia digitata using laboratory animal models. Materials and Methods: The acute oral toxicity (LD 50) of the extract was determined in mice, while the antidiarrhoeal effect of different doses of the extract was evaluated in both mice and rats. The effect of the extract at doses of 300, 700 and 1000 mg/kg were tested against intestinal transit time, magnesium sulphate-induced gastrointestinal motility test and castor oilinduced diarrhoea in mice. Similarly, the extract at the doses mentioned above was evaluated against castor-oil induced enteropooling in rats. Results: The methanol extract of A. digitata was shown to have no toxic effect in mice at doses up to 5000 mg/kg. At doses of 300 and 700 mg/kg the extract significantly (p&lt;0.01) decrease intestinal transit time in mice. In addition, the extract at doses of 300, 700 and 1000 mg/kg, significantly (p&lt;0.05) lowered diarrhoea induced by magnesium sulphate and castor oil in mice. Similarly, the extract produced a significant (p&lt;0.05) inhibition of enteropooling caused by castor oil in rats. The antidiarrhoeal effect of the extract was shown to be dose-dependent
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