Nanocorona Formation between Foodborne Nanoparticles Extracted from Roast Squid and Human Serum Albumin

Foodborne nanoparticles (FNPs) produced by roasting have attracted the attention of people, owing to their safety risk to body health. Herein, we reported the formation, physicochemical properties, elemental composition, biodistribution, and binding with human serum albumin (HSA) of FNPs extracted from roast squid. The results showed that the FNP size gradually decreased from 4.1 to 2.3 nm as the roasting temperature changed from 190 to 250 °C. The main component elements of FNPs are carbon, oxygen, and nitrogen, and the carbon and nitrogen contents of FNPs increased with the roasting temperature rising. The surface of FNPs contained hydroxyl, amino, and carboxyl functional groups. The FNPs can emit fluorescence in ultraviolet light and show excitation-dependent emission behavior. Furthermore, it was found that the FNPs derived from roast squid could be accumulated in the stomach, intestine, and brain of BALB/c mice after oral feeding. Static fluorescence quenching of HSA was found by the Stern–Volmer equation and ultraviolet–visible spectrum analysis after interaction with the FNPs. After the addition of FNPs, the α-helix content of HSA decreased and the morphological height of HSA increased, which indicated that the FNPs could cause structural changes in HSA. The atomic force microscopy characterization showed the formation of nanocorona between FNPs and HSA

Preparation of Polysaccharide–Protein Hydrogels with an Ultrafast Self-Healing Property as a Superior Oral Delivery System of Probiotics

Oral administration of probiotic supplements can effectively regulate intestinal disorders. However, harsh gastrointestinal conditions greatly limit the bioavailability of probiotics. In this work, biomass-derived polysaccharide–protein hydrogels (Dex-sBSA hydrogels) were constructed as an oral probiotic delivery system. The hydrogel encapsulation significantly promoted the growth and proliferation of probiotics and protected them from gastric acid, bile salts, reactive oxygen species, and antibiotics. In vivo experiments demonstrated that the hydrogel encapsulation significantly enhanced the bioavailability of probiotics, of which the cell number in the intestine, colon, and cecum was 35 times, 8 times, and 203 times higher than the free one, respectively. Attributed to the superior ultrafast self-healing property, the Dex-sBSA hydrogel successfully prevented the probiotics from quick elimination and prolonged the retention time in the gut, providing great possibilities for colonization and proliferation. These results clearly indicate the great potential of the Dex-sBSA hydrogel as a superior oral delivery system for probiotics

A Europium(III) Complex as an Efficient Singlet Oxygen Luminescence Probe

A new europium(III) complex, [4‘-(10-methyl-9-anthryl)-2,2‘:6‘,2‘ ‘-terpyridine-6,6‘ ‘-diyl]bis(methylenenitrilo) tetrakis(acetate)−Eu3+, was designed and synthesized as a highly sensitive and selective time-gated luminescence probe for singlet oxygen (1O2). The new probe is highly water soluble with a large stability constant of ∼1021 and a wide pH available range (pH 3−10), and can specifically react with 1O2 to form its endoperoxide (EP-MTTA−Eu3+) with a high reaction rate constant at 1010 M-1 s-1, accompanied by the remarkable increases of luminescence quantum yield from 0.90% to 13.8% and lifetime from 0.80 to 1.29 ms, respectively. The wide applicability of the probe was demonstrated by detection of 1O2 generated from a MoO42-/H2O2 system, a photosensitization system of 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) (TMPyP), and a horseradish peroxidase catalyzed aerobic oxidation system of indole-3-acetic acid (IAA). In addition, it was found that the new probe could be easily transferred into living HeLa cells by incubation with TMPyP. A time-gated luminescence imaging technique that can fully eliminate the short-lived background fluorescence from TMPyP and cell components has been successfully developed for monitoring the time-dependent generation of 1O2 in living cells

Effect of hot-air oven dehydration process on water dynamics and microstructure of apple (<i>Fuji</i>) cultivar slices assessed by LF-NMR and MRI

Non-destructive analysis of water dynamics during drying is of importance for quality control of food products. In this study, different water dynamics and migration in Fuji apple slices dried at various hot-air oven temperatures, i.e. 50, 60, 70, and 80 °C and air velocity at 0.2 m/s were monitored using low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI). Multi-exponential fitting of the transverse relaxation T2 parameter demonstrated four distinct water peaks in all samples corresponding to strongly bound, lightly bound, entrapped/immobilized, and free water as follows: T21 in the range of 0.01–1 ms, T22 in the range of 1–10 ms, T23 in the range of 10–100 ms, and T24 in the range of 100–1000 ms, respectively. The water content was measured and analyzed by the traditional technique using the oven drying method. The overall results were highly significant, depicting that the transverse relaxation times T24, signal per mass of the free water A24, and water content significantly decreased (pL*, a*, b*) and shear force (SF) curves increased with extended drying. Furthermore, good correlations were observed between the LF-NMR parameters and color, water content, and SF in differently processed samples during the dehydration process. Scanning electron microscopy (SEM) and MRI provided the structural changes and spatial water distributions during the drying process. LF-NMR exhibited great potential in evaluating the various water dynamics and quality of Fuji cultivar apples during the drying process. Abbreviations: AA: ascorbic acid; ANOVA: analysis of variance; CPMG: Carr-Purcell-Meiboom-Gill; DW: distilled water; F: Fuji; LF-NMR: low-field nuclear magnetic resonance; MRI: magnetic resonance imaging; SE: spin-echo; SEM: scanning electron microscopy; SF: shear force; SIRT: simultaneous iterative reconstruction technique; TE: echo time; TR: repetition time.</p

Change of Cell Toxicity of Food-Borne Nanoparticles after Forming Protein Coronas with Human Serum Albumin

Nanoparticles (NPs) can form protein coronas with plasma proteins after entering the biological environment due to their surface adsorption ability. In this study, the effects of protein coronas of roast squid food-borne nanoparticles (FNPs) with human serum albumin (HSA) on the HepG-2 and normal rat kidney (NRK) cells were investigated. The hydrodynamic diameters of the HSA and HSA-FNPs were 8 and 13 nm, respectively. The cytotoxicity and cell membrane damage of FNPs to HepG-2 cells increased with the increase of roasting temperature. The presence of 4.78 × 10–3 mol/L FNPs increased the numbers of cellular necrosis and prolonged the G2 phase of the cell cycle. The formation of protein coronas of squid FNPs mitigated the autophagy phenomenon by FNPs on HepG-2 cells. Moreover, protein coronas reduced the mitochondrial membrane potential in the HepG-2 and NRK cells and the production of reactive oxygen species caused by FNPs. The abnormal contents of oxidative stress indicators such as glutathione, superoxide dismutase, malondialdehyde, and catalase in HepG-2 and NRK cells induced by FNPs were alleviated due to the presence of HSA. These results suggested that the protein coronas formed by HSA on FNPs mitigated the cytotoxicity compared with the bare FNPs, thus providing insights into the interaction of squid FNPs with HSA

Procyanidins-Loaded Complex Coacervates for Improved Stability by Self-Crosslinking and Calcium Ions Chelation

The purpose of this work was to develop a facile strategy based on self-crosslinking between the core and wall materials in the coacervation system for effective procyanidins (PCs) encapsulation. The coacervates were constructed through the interaction of bioactive PCs, gelatin, and sodium alginate, followed by forming cationic bridge of sodium alginate-calcium ions to improve the stability of PCs. When the concentration of PCs and calcium ions were 6.25 and 0.24 mg/mL, respectively, the PC-loaded coacervates showed spherical shape with a size about 150 nm, and the microcapsulation efficiency and yield was 81.19 ± 1.47 and 87.86 ± 2.67%, respectively. The photothermal stability of PCs was effectively improved by embedding them in coacervates. The decrease of mitochondrial membrane potential in PC-12 cells induced by H2O2 was significantly inhibited by PC coacervates, demonstrating an improved protection effect of PCs after being encapsulated in coacervates

Universal existence of fluorescent carbon dots in beer and assessment of their potential toxicity

Nanosized materials may produce adverse physiological effects or potential health risks due to their unique physical and chemical properties. Herein, the universal presence of fluorescent carbon dots (CDs) in commercial beers was confirmed through a systematic survey. The beer CDs were roughly spherically shaped in appearance and emitted bright blue fluorescence under ultraviolet light with quantum yields (QYs) ranging from 1.42% to 3.92%. Furthermore, digestion, biodistribution, and cytotoxicity assessments of CDs from Snow beer were conducted as an example. The CDs were significantly quenched during in vitro digestion. The dynamic distribution of CDs in mice showed that they easily accumulated in the intestine and liver, and more importantly, the beer CDs were found in the brain, which indicated that they were able to cross the blood–brain barrier. Acute toxicity of the beer CDs was evaluated using BALB/c mice, and the results revealed that the biochemical parameters of mice after administration of a single dose of 2 g kg−1 body weight were almost same as those of the control groups. Histological analysis showed no obvious organ damage in the tested mice. The in vitro results indicated that CDs dispersed onto both the cell membrane and the cytoplasm of MC3T3-E1 cells, alter the cell cycle progression, and caused cell apoptosis at high doses. This work reports the potential risk of CDs in beer and provides valuable information regarding CDs in food.</p

Characterization of Endogenous Nanoparticles from Roasted Chicken Breasts

Emergence of endogenous nanoparticles in thermally processed food has aroused much attention due to their unique properties and potential biological impact. The aim of this study was to investigate the presence of fluorescence nanoparticles in roasted chicken breasts, elemental composition, physicochemical properties, and their molecular interaction with human serum albumin (HSA). Transmission electron microscopy analysis revealed that the foodborne nanoparticles from roasted chicken were nearly spherical with an average particle size of 1.7 ± 0.4 nm. The elemental analysis of X-ray photoelectron spectroscopy showed the composition of nanoparticles as 47.4% C, 25.8% O, and 26.1% N. The fluorescence of HSA was quenched by the nanoparticles following a static mode, and the molecular interaction of nanoparticles with HSA was spontaneous (Δ<i>G</i>⁰ < 0), where hydrogen bonding and van der Waals forces played an important role during HSA-nanoparticles complex stabilization through thermodynamic analysis by isothermal titration calorimetry. The principal location of the nanoparticles binding site on HSA was primarily in site I as determined by site-specific marker competition. The conformational of HSA was also changed and α-helical structure decreased in the presence of nanoparticles. Our studies revealed that fluorescent nanoparticles were produced after roasting of chicken breast at 230 °C for 30 min for the first time. The obtained nanoparticles can interact with HSA in a spontaneous manner, thus providing valuable insight into foodborne NPs as well as their effects to human albumin protein

Tuning the Microstructures of Electrospray Multicore Alginate Microspheres for the Enhanced Delivery of Astaxanthin

Multicore alginate microspheres (MCPs) have been demonstrated as promising carriers for bioactive substances. Herein, the influence of the size of the inner core on the bioaccessibility of astaxanthin (AST) was investigated using both in vitro and in vivo methods. MCPs with different inner core sizes were fabricated in which the oil-in-water emulsion with different oil droplet sizes was embedded in alginate microspheres (AST@MCPs) via the electrospray technology. The AST@MCPs appeared as a uniform sphere with an average size of 300 μm. The AST encapsulation efficiency in the AST@MCPs was determined to be more than 68%, which was independent of the inner core size. The bioaccessibility of AST increased from 38.3 to 83.2% as the size of the inner core decreased. Furthermore, the anti-inflammatory activity of AST@MCPs after in vitro simulated digestion was evaluated by LPS-induced RAW264.7 cells. The results suggested that AST@MCPs with a smaller inner core size exhibited a stronger anti-inflammatory activity, which further proved the results obtained from in vitro simulated digestion. As expected, the oral administration of AST@MCPs significantly mitigated colitis symptoms in DSS-induced ulcerative colitis mice. Compared with AST@MCPs with larger inner cores, AST@MCPs with smaller inner cores reflect stronger anti-inflammatory activity in vivo. These results suggested that the bioaccessibility of AST in MCPs increased significantly with the decrease in the inner core size, which may be attributed to the rapid formation of micelles in the intestine. This work provides a simple and efficient strategy to prepare microspheres for the enhanced delivery of AST, which has important implications for the design of health-promoting foods

Blue Electroluminescence from InN@SiO₂ Nanomaterials

We report here on the blue electroluminescence from InN@SiO2 nanomaterials prepared by growing InN semiconductor nanocrystals on silica nanoparticles. A blue electroluminescence was observed at 460 nm from a light-emitting device using InN@SiO2 nanomaterials as emission layer. The blue emission remained almost unchanged at different driving voltages. The electroluminescence measurements on various control devices prove that the blue emission is indeed from the InN@SiO2 nanomaterials. The InN@SiO2-based single-layer device emitted almost pure blue light with CIE coordinates of 0.18, 0.23, demonstrating a potential application in electroluminescent devices