Effect of rapamycin and CQ on the mTOR pathway in the hippocampus.

<p>(A) A representative graph of western blot analysis of p-mTOR and mTOR expression in the hippocampus of each group, n = 6. (B) A representative graph of western blot analysis of p-S6K1 and S6K1 expression in the hippocampus of each group, n = 6. Data are presented as mean ± SD. *<i>P</i> < 0.05 or **<i>P</i> < 0.01, ^#<i>P</i> > 0.05.</p

Confirmation of the differences in autophagy in the hippocampal neurons with electron microscopy.

<p>(A)Representative electron micrographs showing autophagic vacuoles in the hippocampus of aged rats in each group, n = 3. (B) Quantitative analysis of the number of autophagic vacuoles per neuron in each group. 3 rats were in each group, and ten cells were examined for each rat. The data are presented as the means±SD. Data are presented as mean ± SD. *<i>P</i> < 0.05 or **<i>P</i> < 0.01.</p

Effect of rapamycin and CQ on sevoflurane-induced memory impairment.

<p>(A) Escape latency (time to find the hidden platform) plotted against training day. Repeated measures ANOVA followed by a post hoc Bonferroni multiple comparison test: CON vs. SEV, <i>P</i> = 0.0002, <i>F</i> = 15.47, n = 9; SEV vs. SEV+RAP, <i>P</i> = 0.0155, <i>F</i> = 6.19, n = 9; SEV vs. SEV+CQ, <i>P</i> = 0.0412, <i>F</i> = 4.55, n = 9; CON vs. RAP, <i>P</i> = 0.0569, <i>F</i> = 0.33, n = 9; CON vs. CQ, <i>P</i> = 0.21, <i>F</i> = 15.47, n = 9. (B) The platform crossing times during the probe trial of the MWM test. (C) The time spent in the target quadrant during the probe test of MWM. Data are presented as mean ± SEM (n = 9). *<i>P</i> < 0.05, **<i>P</i> < 0.01, ^#<i>P</i> > 0.05.</p

Confirmation of the differences in autophagy in the hippocampal neurons.

<p>(A) Comparison of LC3 and p62 expression in the hippocampus of aged rats. β-actin was used as an endogenous control. (B) A statistical chart of the relative optical density of p62/β-actin in each group, n = 6. (C) A statistical chart of relative optical density of LC3-II/LC3- I in each group, n = 6. (D) Relative expression of p62 mRNA in each group, n = 6. (E) Relative expression of LC3 mRNA in each group, n = 6. *<i>P</i> < 0.05, **<i>P</i> < 0.01 or ^# <i>P</i> >0.05.</p

Effect of rapamycin and CQ on sevoflurane-induced apoptosis in the hippocampus analyzed by using TUNEL staining.

<p>(A) Representative photomicrographs showing TUNEL-positive cells in the hippocampus of aged rats in each group, n = 3. (B) A statistical chart of the ratio of TUNEL-positive cells/total cells, 3 rats in each group and 5 fields were observed in each rat. Data are presented as mean ± SD,*<i>P</i> < 0.05 or **<i>P</i> < 0.01, ^#<i>P</i> > 0.05.</p

Effects of sevoflurane exposure on physiological parameters of arterial blood gas analysis of aged rats.

<p>Effects of sevoflurane exposure on physiological parameters of arterial blood gas analysis of aged rats.</p

Effect of rapamycin and CQ on sevoflurane-induced apoptosis in the hippocampus.

<p>(A) Comparison of Bax, Bcl-2, and cleaved caspase3 expression in the hippocampus of aged rats in each group. β-actin was used as an endogenous control. (B) A statistical chart of the relative optical density of cleaved caspase3/β-actin in each group, n = 6. (C) A statistical chart of relative optical density of Bax/β-actin in each group, n = 6. (D) A statistical chart of relative optical density of Bcl-2/β-actin in each group, n = 6. Data are presented as mean ± SD. *<i>P</i> < 0.05 or **<i>P</i> < 0.01, ^#<i>P</i> > 0.05.</p

Datasheet1_Patient perceptions and preferences of minimally invasive treatment modalities in varicose veins: a cross-sectional survey.docx

PurposeMinimally invasive therapies (MIT) have gained popularity due to their capacity to reduce trauma, enhance aesthetic outcomes, and shorten recovery periods. This article explores patients’ perceptions and preferences regarding MIT for varicose veins (VVs) while analyzing associated influencing factors to provide a better understanding.Patients and methodsA cross-sectional survey at Zhejiang Rongjun Hospital was performed from January 2022 to June 2023, involving 305 participants with VVs. The questionnaire assessed patient demographics, VVs severity, prior treatment experiences, and treatment preferences. Statistical analyses, including chi-square and Kruskal-Wallis tests, were conducted to explore the correlations between patient characteristics, treatment preferences, and factors influencing these choices.ResultsNearly half of the participants (44.3%) lacked information on any surgical options, whereas a slight majority (55.7%) possessed familiarity with at least one treatment modality, and only 9.8% knew of all six treatment methods presented. Patient surveys discerned that the majority (68.5%) declared an inadequate grasp of treatment methodologies to articulate a treatment preference. Among the 96 patients who made a treatment choice, 24.0% opted for traditional surgery, while 76.0% chose MIT and a higher preference for MIT among male patients compared to female patients (p = 0.006). The patients preferred treatment options for VVs significantly affected by vascular surgeon recommendations and the number of follow-up visits (r = 0.129, p = 0.024; r = 0.122, p = 0.033).ConclusionThe study highlights limited awareness of MIT among Chinese patients with VVs. The insights emphasize the influential role of vascular surgeons’ recommendations and suggest a growing predilection for less invasive treatments due to their advantages in recovery and aesthetics. Provider-patient communication, including education about available treatments and shared decision-making, is essential to align treatment plans with patient expectations and improve outcomes.</p

Identification of cancer-related miRNA-lncRNA biomarkers using a basic miRNA-lncRNA network

The table contains the expression data of miRNAs and lncRNAs in the BRCA, KIRC, LUAD, LUSC, PRAD and THCA

The number of cancer samples remaining after preprocessing.

<p>The number of cancer samples remaining after preprocessing.</p