14 research outputs found

    Construction of rabbit corneal epithelium tissue-engineered with acellular conjunctiva matrix.

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    <p>Rabbit primary limbal epithelial cells proliferated well at day 7 (A). Trypan blue-alizarin red staining showed that the cells maintained live activity and grew to confluence (B). The cells could be labeled with CM-Dil (red fluorescence) (C) and stained positive with anti-K3/12 (D). Negative control lacks primary antibody (E). The rabbit corneal epithelial cells formed a 2–3 epithelial layer structure after culture for 14 days, as confirmed by H.E. staining (F) and TEM observation (G). There were tight junctions between cells and the aCM scaffold (H). Scale bar: 100 µm (D, E), 50 µm (B, C, F), 5 µm (G) and 1 µm (H).</p

    Construction of a rabbit limbal stem cell deficiency model.

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    <p>Representative slit-lamp photograph of rabbit cornea showed significant opacity and neovascularization after 1 month (A). PAS staining in which goblet cells are easily observed in corneal impression cytology (B). Signet ring goblet cells (arrow) were also revealed by H.E. staining of corneal sections (C). Scale bar: 10 µm (B) and 50 µm (C).</p

    Morphological changes in acellular conjunctiva matrix and denuded amniotic membrane after 0.25% collagenase digestion.

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    <p>Morphological changes in acellular conjunctiva matrix and denuded amniotic membrane after 0.25% collagenase digestion.</p

    Biocompatibility of acellular conjunctiva matrix in vivo.

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    <p>Representative slit-lamp photographs just after intracorneal transplantation (A) and one month later (B). H.E. staining showed that transplanted aCM (arrow) adapted well to the host corneal stroma, with no evidence of inflammatory cells or stromal edema. Scale bar: 100 µm (C) and 50 µm (D).</p

    Corneal H and E staining and impression cytology after transplantation of tissue-engineered corneal epithelium.

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    <p>Restoration of corneal epithelium began at day 7 after transplantation with reconstructed corneal epithelium on aCM (A). Normal morphology was observed at day 30 (B) with no goblet cells (C), whereas the group that received reconstructed corneal epithelium on dAM exhibited defective corneal epithelium at day 7 (D) and goblet cells (arrow) were still observed at day 30 (E, F). Scale bar: 100 µm (A, B, D, E) and 10 µm (C, F).</p

    The effect of acellular conjunctiva matrix extracts on the proliferation of human corneal epithelial cells.

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    <p>The effect of acellular conjunctiva matrix extracts on the proliferation of human corneal epithelial cells.</p

    Scores of the corneal surface over time in all groups (corneal haze/neovascularization/sum).

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    <p>Scores of the corneal surface over time in all groups (corneal haze/neovascularization/sum).</p

    Macroscopic view of the acellular conjunctiva matrix.

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    <p>The aCM (B, E) were more transparent than the normal conjunctiva matrix (A, D). The transparent characteristics were not affected after the sterilization with γ-irradiation (C, F).</p

    Postoperative tracking of donor cells on the recipient cornea.

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    <p>More donor cells were detected on peripheral region of the rabbit cornea transplanted with aCM (A) compared with that transplanted with dAM (B) at days 7 and 30. At day 30, more donor cells had migrated into the central cornea region transplanted with aCM-based corneal epithelium (A), whereas few cells were detected in the central cornea transplanted with dAM-based corneal epithelium (B). Scale bar: 100 µm.</p

    Effect of macrophage depletion on myofibroblast differentiation.

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    <p>Corneal flat mount staining after 14 days of penetrating keratoplasty showed that α-SMA was strongly expressed in the junction area between the graft and recipient cornea (A) and parallel to the corneal surface (B, C) in the PBS-LIP-injected mice, while in the Cl<sub>2</sub>MDP-LIP-treated mice, α-SMA expression was significantly lower (D), and the fibers in the junction area were more disorganized than in the control mice (E,F).</p
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