14 research outputs found

    Additional file 1: of Basic leucine zipper transcription factor SlbZIP1 mediates salt and drought stress tolerance in tomato

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    Figure S1. Relative expression profiles of SlbZIP07, SlbZIP10 and SlbZIP39 in the leaves of WT and SlbZIP1-RNAi lines under normal conditions. (DOCX 77 kb

    Anthocyanin Accumulation and Molecular Analysis of Correlated Genes in Purple Kohlrabi (Brassica oleracea var. <i>gongylodes</i> L.)

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    Kohlrabi (Brassica oleracea var. <i>gongylodes</i> L.) is an important dietary vegetable cultivated and consumed widely for the round swollen stem. Purple kohlrabi shows abundant anthocyanin accumulation in the leaf and swollen stem. Here, different kinds of anthocyanins were separated and identified from the purple kohlrabi cultivar (Kolibri) by high-performance liquid chromatography–electrospray ionization tandem mass spectrometry. In order to study the molecular mechanism of anthocyanin biosynthesis in purple kohlrabi, the expression of anthocyanin biosynthetic genes and regulatory genes in purple kohlrabi and a green cultivar (Winner) was examined by quantitative PCR. In comparison with the colorless parts in the two cultivars, most of the anthocyanin biosynthetic genes and two transcription factors were drastically upregulated in the purple tissues. To study the effects of light shed on the anthocyanin accumulation of kohlrabi, total anthocyanin contents and transcripts of associated genes were analyzed in sprouts of both cultivars grown under light and dark conditions

    Additional file 3: of Basic leucine zipper transcription factor SlbZIP1 mediates salt and drought stress tolerance in tomato

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    Table S1. Gene products of annotated genes or with sequence similarity showing at least 2-fold change in transcript abundance (p < 0.05) in leaves of SlbZIP1-RNAi line Ri2 compared with WT plants. (XLSX 76 kb

    Multiple sequence alignment of the amino acid sequences of SlDEAD30, SlDEAD31, AtRH9, and AtRH36 proteins.

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    <p>Identical or similar amino acids are shaded in black and grey, respectively. The locations of the nine conserved helicase motifs are indicated on top of the sequences and DEAD domains are shown in the box. The conserved phenylalanine residue is indicated by an inverted triangle. SlDEAD30 (KJ739798), SlDEAD31 (KJ713393), AtRH9 (NM_125492), and AtRH36 (NM_101494).</p

    Phylogenetic tree and reported or predicted functions of SlDEAD30, SlDEAD31, and other known plant DEAD-box proteins.

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    <p>The phylogenetic tree analysis was constructed with MEGA 5.2 software using the neighbor-joining method and the following parameters: bootstrap analysis of 1000 replicates, poisson model and pairwise deletion. The numbers at the nodes indicate the bootstrap values. SlDEAD30 and SlDEAD31 are marked with a black triangle and a red triangle, respectively. Accession numbers and corresponding references for the proteins used are as follows: <i>Solanum lycopersicum</i>: SlDEAD30, KJ739798; SlDEAD31, KJ713393. <i>Arabidopsis thaliana</i>: AtRH3, NM_001036866 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref028" target="_blank">28</a>]; AtRH9, NM_125492 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref021" target="_blank">21</a>]; AtRH22, NM_104691 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref026" target="_blank">26</a>]; AtRH36, NM_101494 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref025" target="_blank">25</a>]; AtRH52, NM_115719 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref026" target="_blank">26</a>]; STRS1, AY080680 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref016" target="_blank">16</a>]; STRS2, AY035114 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref016" target="_blank">16</a>]; LOS4, BT002444 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref019" target="_blank">19</a>]; RCF1, BT002030 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref054" target="_blank">54</a>]; AtCAF, AF187317 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref027" target="_blank">27</a>]; AtHEN2, AY050658 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref055" target="_blank">55</a>]. UPF1, AF484122 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref056" target="_blank">56</a>]. <i>Oryza sativa</i>: OsBIRH1, Q0DVX2 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref017" target="_blank">17</a>]; OsABP, LOC_Os06g33520 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref057" target="_blank">57</a>]; <i>Hordeum vulgare</i>: HVD1, AB164680 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref058" target="_blank">58</a>]; <i>Glycine max</i>: GmRH, FJ462142 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref059" target="_blank">59</a>]. <i>Apocynum venetum</i>: AvDH1, EU145588 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133849#pone.0133849.ref015" target="_blank">15</a>].</p
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