78 research outputs found
The influence of IAC capacity after reuse.
<p>The error bars indicate IAC capacity deviations with three columns.</p
Standard curves by ELISA for diniconazole.
<p>ELISA concentrations were the values of three measurements. The error bars indicate standard deviations (n = 3). .</p
Influence of matrix dilution on the reliability of the diniconazole ELISA.
a<p>tomato and pear samples were diluted (2.5, 5, 10, and 15-fold) with PBS containing 20% methanol.</p
Regression curve of GC versus ELISA methods for determination of diniconazole in water, pear and tomato samples.
<p>ELISA concentrations were the mean values of triplicate measurements.</p
HPLC chromatography of pear samples spiked with 0.1 mg kg<sup>−1</sup> diniconazole (a: non-purified, b: purified by IAC), blank pear sample (c: purified by IAC) and standard solution of 1 mg kg<sup>−1</sup> diniconazole (d).
<p>Peak identifications: (A). impurity, (B). diniconazole.</p
Diniconazole recovery from the spiked samples by HPLC and the ELISA.
<p>Diniconazole recovery from the spiked samples by HPLC and the ELISA.</p
Quantitative Shotgun Proteomics Associates Molecular-Level Cadmium Toxicity Responses with Compromised Growth and Reproduction in a Marine Copepod under Multigenerational Exposure
In
this study, the copepod <i>Tigriopus japonicus</i> was exposed
to different cadmium (Cd) treatments (0, 2.5, 5, 10,
and 50 μg/L in seawater) for five generations (F0–F4),
followed by a two-generation (F5–F6) recovery period in clean
seawater. Six life-history traits (survival, developmental time of
nauplius phase, developmental time to maturation, number of clutches,
number of nauplii/clutch, and fecundity) were examined for each generation.
Metal accumulation was also analyzed for generations F0–F6.
Additionally, proteome profiling was performed for the control and
50 μg/L Cd-treated F4 copepods. In F0–F4 copepods, Cd
accumulated in a concentration-dependent manner, prolonging the development
of the nauplius phase and maturation and reducing the number of nauplii/clutch
and fecundity. However, during F5–F6, Cd accumulation decreased
rapidly, and significant but subtle effects on growth and reproduction
were observed only for the highest metal treatment at F5. Proteomic
analysis revealed that Cd treatment had several toxic effects including
depressed nutrient absorption, dysfunction in cellular redox homeostasis
and metabolism, and oxidative stress, resulting in growth retardation
and reproduction limitation in this copepod species. Taken together,
our results demonstrate the relationship between molecular toxicity
responses and population-level adverse outcomes in <i>T. japonicus</i> under multigenerational Cd exposure
Development of Immunoassays for Detecting Clothianidin Residue in Agricultural Products
Two
enzyme-linked immunosorbent assays (ELISAs) based on polyclonal
antibodies (PcAbs) for clothianidin are described: colorimetric detection
format (ELISA) and pattern of chemiluminescent assay (CLEIA). Clothianidin
hapten was synthesized and conjugated to bovine serum albumin (BSA)
and ovalbumin (OVA) to produce immunogen and coating antigen. Anticlothianidin
PcAbs were obtained from immunized New Zealand white rabbits. Under
optimal conditions, the half-maximal inhibition concentration (IC<sub>50</sub>) and the limit of detection (LOD, IC<sub>20</sub>) of clothianidin
were 0.046 and 0.0028 mg/L for the ELISA and 0.015 and 0.0014 mg/L
for the CLEIA, respectively. There were no obvious cross-reactivities
of the antibodies with its analogues except for dinotefuran. Recoveries
of 76.4–116.4% for the immunoassays were achieved from spiked
samples. The results of immunoassays for the spiked and authentic
samples were largely consistent with gas chromatography. Therefore,
the proposed immunoassays would be convenient and satisfactory analytical
methods for the monitoring of clothianidin in agricultural products
Expression of miR-194-5p, miR-192-5p, and miR-215-5p in human colon cell lines.
<p>The level of expression is given in sequence counts per million.</p
- …