Additional file 1: of Evaluation of differential qPE9-1/DEP1 protein domains in rice grain length and weight variation

Table S1. Primers used in this study. (DOC 32 kb

Additional file 1: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Figure S1. Pollen viability and embryo structures in the RGB1/rgb1 heterozygous mutant lines. (a) Pollen viability in the WT and RGB1/rgb1 heterozygous mutants (Bar = 200 μm). The pollen grains were stained with 1% I2–KI and imaged with a Leica DM 1000 light microscope. (b) Median longitudinal sections of WT and RGB1/rgb1 heterozygous mutant seeds (18 days after flowering); a plumule, b radicle. (DOCX 3952 kb

Additional file 8: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Table S3. Number of differentially expressed genes identified in the transcriptome analysis. (DOCX 13 kb

Additional file 4: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Figure S4. Stages of seed germination and early postgermination seedlings of rice. (DOCX 4775 kb

Additional file 5: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Figure S5. Comparison of gene expression determined by qRT-PCR and RNA-seq and relative expression of auxin signaling-related genes in the embryos of the WT and rgb1–2 mutants at 2 and 3 days after germination. (a) Comparison of relative gene expression levels determined by RNA-seq and qRT-PCR. Pearson’s test indicated a strong correlation between the two techniques (r = 0.85; p < 0.05). (b-d) Relative expression of auxin-related family genes. Gene expression was determined in the WT and rgb1–2 mutant embryos at 2 and 3 days after germination. (b) OsIAA; (c) OsARF; (d) OsPIN (DOCX 476 kb

Additional file 7: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Table S2. Genotypes of the seeds in the T0-, T1-, and T2-generation transgenic seedlings. (XLSX 16 kb

Additional file 9: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Table S4. GO, COG, and KEGG classification tables. (XLSX 77 kb

Additional file 2: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Figure S2. Phenotypes and genotypes of the rgb1 mutants; relative expression of G protein genes in the embryos of the WT and rgb1 mutants. (a-d) DNA sequences and phenotypes of the WT and rgb1 mutants. (a) WT, (b) rgb1–1, (c) rgb1–2, (d) rgb1–3. Mutations are shown in red. (e) Relative expression of RGB1 in the embryos of the WT and the rgb1–1 and rgb1–2 mutant lines, as determined by qRT-PCR. The forward primer used in the qRT-PCR assays was the RGB1-Cas9 target sequence. (f-h) Relative expression of G protein family genes in the embryos of the WT and the rgb1–1 and rgb1–2 mutant lines from (f) 1-day-old seedlings, (g) 2-day-old seedlings, and (h) 3-day-old seedlings. (DOCX 2619 kb

Additional file 11: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Table S6. Gene expression involved in various response/regulatory pathways. (XLSX 82 kb

Additional file 10: of The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice

Table S5. Genes specifically expressed in the SAM, vascular tissues and developing roots. (XLSX 13 kb