Changes in the abundance of GmMAN1 in abscission zone (Z) and non-abscission zone (N) in soybean petiole explants.

<p>Molecular masses (kDa) are indicated on the right. Equal amounts (100 µg) of protein were loaded on each lane.</p

Ultrastructural changes of apoptotic neurons in sevoflurane group.

<p>These degenerating neurons showed an advanced stage of apoptosis, with chromatin clumping, condensation and margination (arrow), (a) a control rat. (b) a sevoflurane treated rat, Scale bar = 2 µm.</p

Sevoflurane induced apoptosis of cells in the hippocampus of aged rats.

<p>Apoptosis was examined by the TUNEL method. Upper: Photomicrographs of TUNEL-positive cells. (a) Control group. (b) Sevoflurane group. (c) Number of TUNEL-positive cells in each group. Data are presented as mean ± SD. *<i>P</i><0.05, <i>vs</i> control group.</p

Changes in the abundance of <i>GmMAN1</i> transcripts in abscission zone (Z) and non-abscission zone (N) in soybean petiole explants.

<p>Equal amounts (20 µg) of RNA were loaded on each lane.</p

Changes in endo-β-mannanase activity in the leaves (A), stem (B) and roots (C) in soybean plants after wounding.

<p>Plants of 17-d-old were wounded by removing half of the leaf blade of the first pair of true leaves. CK: control, unwounded plants. Means of three measurements ±SD.</p

Changes in the abundance of GmMAN1 in the leaves (L), stem (S) and roots (R) in wounded (W) and control (C) soybean plants.

<p>Molecular masses (kDa) are indicated on the right. Equal amounts (100 µg) of protein were loaded on each lane.</p

Changes in the ultrathin-layer isoelectric focusing isoform profiles of endo-β-mannanase in abscission zone (Z) and non-abscission zone (N) of soybean petiole explants.

<p>Isoelectric points (pI) are indicated on the right. Equal amounts (30 µg) of protein were loaded on each lane.</p

The vital signs and arterial blood gas during anesthetics treatment.

*<p><i>P</i>>0.05 <i>vs</i> sham anesthesia rats.</p><p>HR:heart rate, MAP: mean artery pressure, PaCO₂: partial pressure of carbon dioxide in artery, SPO₂: saturation of arterial blood oxygen.</p

Changes in the ultrathin-layer isoelectric focusing isoform profiles of endo-β-mannanase in abscission zone (Z) and non-abscission zone (N) in intact soybean plants treated with 0.1% (v/v) ethephon.

<p>Isoelectric point (pI) is indicated on the right. Equal amounts (30 µg) of protein were loaded on each lane.</p

Immunohistochemistry of CHOP and Caspase-12 in hippocampus.

<p>(a) CHOP immunostain in control group ×400. (b) CHOP immunostain in sevoflurane group ×400. (c) Caspase-12 immunostain in control group ×400. (d) Caspase-12 immunostain in sevoflurane group ×400. (e) Numbers of CHOP and Caspase-12-positive cells. (f) Optical density of CHOP and Caspase-12-positive cells. Data are presented as mean ± SD. *<i>P</i><0.05, <i>vs</i> the control group.</p