第698回 千葉医学会例会・第18回 肺癌研究施設例会 40.

<div><p>Dietary protein restriction is not only beneficial to health and longevity in humans, but also protects against air pollution and minimizes feeding cost in livestock production. However, its impact on amino acid (AA) absorption and metabolism is not quite understood. Therefore, the study aimed to explore the effect of protein restriction on nitrogen balance, circulating AA pool size, and AA absorption using a pig model. In Exp.1, 72 gilts weighting 29.9 ± 1.5 kg were allocated to 1 of the 3 diets containing 14, 16, or 18% CP for a 28-d trial. Growth (n = 24), nitrogen balance (n = 6), and the expression of small intestinal AA and peptide transporters (n = 6) were evaluated. In Exp.2, 12 barrows weighting 22.7 ± 1.3 kg were surgically fitted with catheters in the portal and jejunal veins as well as the carotid artery and assigned to a diet containing 14 or 18% CP. A series of blood samples were collected before and after feeding for determining the pool size of circulating AA and AA absorption in the portal vein, respectively. Protein restriction did not sacrifice body weight gain and protein retention, since nitrogen digestibility was increased as dietary protein content reduced. However, the pool size of circulating AA except for lysine and threonine, and most AA flux through the portal vein were reduced in pigs fed the low protein diet. Meanwhile, the expression of peptide transporter 1 (PepT-1) was stimulated, but the expression of the neutral and cationic AA transporter systems was depressed. These results evidenced that protein restriction with essential AA-balanced diets, decreased AA absorption and reduced circulating AA pool size. Increased expression of small intestinal peptide transporter PepT-1 could not compensate for the depressed expression of jejunal AA transporters for AA absorption.</p></div

Additional file 1 of LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Additional file 1: Figure S1. MAPK1 knockdown promotes apoptosis and inhibits migration and invasion in gastric cancer cells. (A) Cell apoptosis was detected in MKN-45 and MGC-803 cells transfected with sh-MAPK1-1, sh-MAPK1-2 or sh-NC by flow cytometry. (B and C) Cell migration and invasion were measured in MKN-45 and MGC-803 cells transfected with sh-MAPK1-1, sh-MAPK1-2 or sh-NC by transwell assay

Additional file 4 of LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Additional file 4: Figure S4. LINC00483 sponges miR-490-3p to regulate apoptosis, migration and invasion in gastric cancer cells. (A) Cell apoptosis was detected in MKN-45 and MGC-803 cells transfected with miR-NC, miR-490-3p, miR-490-3p and pcDNA or LINC00483 by flow cytometry. (B and C) Cell migration and invasion were measured in MKN-45 and MGC-803 cells transfected with miR-NC, miR-490-3p, miR-490-3p and pcDNA or LINC00483 by transwell assay

Additional file 2 of LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Additional file 2: Figure S2. MAPK1 overexpression reverses the effect of LINC00483 knockdown on cell apoptosis, migration and invasion in gastric cancer cells. (A) Cell apoptosis was detected in MKN-45 and MGC-803 cells transfected with sh-NC, sh-LINC00483-1, sh-LINC00483-1 and pcDNA or MAPK1 by flow cytometry. (B and C) Cell migration and invasion were measured in MKN-45 and MGC-803 cells transfected with sh-NC, sh-LINC00483-1, sh-LINC00483-1 and pcDNA or MAPK1 by transwell assay

Additional file 3 of LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Additional file 3: Figure S3. The effect of LINC00483 on 5 predicted miRNAs. miRcode and TargetScan predicted the miRNAs that have the binding sites of LINC00483 and MAPK1. 5 lowly-expressed miRNAs (miR-183, miR-101-3p, miR-128-3p, miR-129-5p and miR-490-3p) in gastric cancer were selected. The effect of LINC00483 knockdown on these miRNAs’ expression was measured in MKN-45 cells. *P < 0.05

Data_Sheet_1_Evaluating and analyzing student labor literacy in China's higher vocational education: an assessment model approach.PDF

IntroductionThis study addresses the gap in evaluating labor literacy amongst vocational students in China's higher vocational education system. It aims to develop a comprehensive framework for assessing essential labor competencies, thereby contributing to a nuanced understanding of vocational education's role in skill development.MethodsEmploying a multifaceted research methodology, this study integrates questionnaire surveys, econometric analyses, and the Delphi method to assess labor literacy among 749 students from three leading vocational institutions. A pioneering labor literacy assessment model is introduced: S = 0.5466B1+0.1816B2+0.1623B3+0.1095B4, where S denotes the overall labor literacy score. Here, B1 represents labor concepts, B2 denotes habits and qualities, B3 signifies knowledge and skills, and B4 encapsulates emotions and attitudes, illustrating a comprehensive approach to measuring labor literacy.ResultsOur findings reveal pronounced disparities in labor literacy across the identified dimensions, with particular deficiencies in labor concepts. The study also identifies six determinants—gender, political profile, academic performance, internship and training base utilization, inclination towards innovation and entrepreneurship, and labor education evaluation mechanisms—that significantly influence labor literacy outcomes.DiscussionHighlighting the imperative for a contextually informed and holistic approach to labor literacy, this study's insights advocate for educational strategies that are both aligned with labor market demands and cognizant of socio-cultural nuances. The developed assessment model not only propels the theoretical discourse in vocational education forward but also provides a pragmatic guide for educators and policy makers, aiming to mitigate disparities and enhance labor competencies through refined educational practices.</p

Additional file 2 of Screening drug target combinations in disease-related molecular networks

A total of 23 reaction constants was taken from experimental values, while the others were obtained by fitting the calculated production of LTB4. (PDF 119 kb

Table_1_Evaluating and analyzing student labor literacy in China's higher vocational education: an assessment model approach.XLSX

IntroductionThis study addresses the gap in evaluating labor literacy amongst vocational students in China's higher vocational education system. It aims to develop a comprehensive framework for assessing essential labor competencies, thereby contributing to a nuanced understanding of vocational education's role in skill development.MethodsEmploying a multifaceted research methodology, this study integrates questionnaire surveys, econometric analyses, and the Delphi method to assess labor literacy among 749 students from three leading vocational institutions. A pioneering labor literacy assessment model is introduced: S = 0.5466B1+0.1816B2+0.1623B3+0.1095B4, where S denotes the overall labor literacy score. Here, B1 represents labor concepts, B2 denotes habits and qualities, B3 signifies knowledge and skills, and B4 encapsulates emotions and attitudes, illustrating a comprehensive approach to measuring labor literacy.ResultsOur findings reveal pronounced disparities in labor literacy across the identified dimensions, with particular deficiencies in labor concepts. The study also identifies six determinants—gender, political profile, academic performance, internship and training base utilization, inclination towards innovation and entrepreneurship, and labor education evaluation mechanisms—that significantly influence labor literacy outcomes.DiscussionHighlighting the imperative for a contextually informed and holistic approach to labor literacy, this study's insights advocate for educational strategies that are both aligned with labor market demands and cognizant of socio-cultural nuances. The developed assessment model not only propels the theoretical discourse in vocational education forward but also provides a pragmatic guide for educators and policy makers, aiming to mitigate disparities and enhance labor competencies through refined educational practices.</p

Additional file 1 of Screening drug target combinations in disease-related molecular networks

The ordinary differential equations of AA metabolic network was developed. A series of ODEs was established to simulate unicellular behavior, which included 24 initial concentrations and 45 reaction constants. (PDF 130 kb

Synthesis and Optical Properties of Soluble Near-Infrared Arylene Bisthienoimidazole Dyes

Soluble, film-forming near-infrared arylene bisthienoimidazole dyes with a band gap of 1.2–1.3 eV are electrochromic and absorb strongly at the telecommunication wavelengths (1310 and 1550 nm) in the oxidized state