Inhibition of ferrochelatase impairs vascular eNOS/NO and sGC/cGMP signaling - Fig 1

<p>(a) Changes in vascular ferrochelatase (FECH) activity in isolated bovine coronary arteries (BCAs) incubated with difference doses of N-methyl protoporphyrin (NMPP;10⁻⁷, 10⁻⁶ and 10^-5M) for 24 (24-h) and 72 hours (72-h), respectively (n = 6–10). *significant difference from their controls. #significant difference from 24-h group. (b) Original and summarized data for FECH protein expression, normalized to voltage-dependent anion channel (VDAC) in isolated BCAs incubated with 10^-6M NMPP for 24 and 72 hours respectively (n = 3 blots).</p

Changes in vascular heme levels in BCAs cultured with NMPP (10^-6M) for 24 and 72 hours respectively (n = 12).

<p>*significant difference from controls.</p

Vascular wall tension in response to stretch-induced changes in radius (a & d), force development in response to stimulation with 122 mM KCl (HK) and 30 mM KCl (30K) (b & e), and vascular relaxations to isoproterenol (c & f) in BCA rings cultured with NMPP (10^-6M) for 24 (a-c) and 72 (d-f) hours respectively.

<p>(n = 11–14).</p

Vascular protein expression of HSP90 (a; n = 8 for each group) and relaxation response to BAY58-2627 (b; n = 8 for each group) in BCAs in the control condition and cultured with NMPP (10^-6M) for 72 hours.

<p>Vascular protein expression of HSP90 (a; n = 8 for each group) and relaxation response to BAY58-2627 (b; n = 8 for each group) in BCAs in the control condition and cultured with NMPP (10^-6M) for 72 hours.</p

Vascular superoxide production, indicated by 2-hydroxyethidium levels in BCAs cultured with NMPP (10^-6M) for 24 and 72 hours respectively.

<p>n = 8.</p

Changes in vascular relaxations to Acetylcholine (ACh; a & d) and spermine-NONOate (c & f), as well as basal and stimulated release of nitrite/NO (b & e) in BCA rings cultured with NMPP (10^-6M) for 24 (a-c) and 72 (d-f) hours respectively.

<p>(n = 12–15). *significant difference from their controls. #significant difference from baseline controls. † significant difference between the two curves.</p