Additional file 3 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 3: Supplementary File 2. The gene alignments and all parameters for the phylogenetic analysis conducted in this study

Additional file 2 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 2: Supplementary File 1. All of the scripts and parameters used for the genome assemblies produced in this study

Additional file 4 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 4: Table S1. Genome sequencing and assembly statistics. Table S2. Comparisons of Huntiella genome statistics. Table S3. Gene present at the second a-factor pheromone locus

Additional file 1 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 1: Fig. S1. RNA mapping to confirm the single nucleotide deletion in the H. moniliformis MAT1-2-7. Fig. S2. The structure of the Huntiella a-factor pheromone proteins and the sequence of the putative mature repeats. Fig. S3. An alignment of the a1 a-factor pheromone factor genes from all eight Huntiella species considered in this study. Fig. S4. RNA mapping to confirm in frame stop codons in the a1 and a6 a-factor pheromone factor genes from the unisexual H. moniliformis. Fig. S5. An alignment of the a3 a-factor pheromone from all eight Huntiella species considered in this study. Fig. S6. RNA mapping to determine expression of the multiple a-factor pheromone genes from H. abstrusa. Fig. S7. RNA mapping to determine expression of the multiple a-factor pheromone genes from H. omanensis. Fig. S8. RNA mapping to determine expression of the multiple a-factor pheromone genes from H. moniliformis. Fig. S9. The structure of the Huntiella α-factor pheromones from the various Huntiella species along with the sequences of the putative mature repeats

The diversity of the Leotiomycete a-factor pheromone in the Ploettnerulaceae, Drepanopezizaceae, Mollisiaceae, Erysiphaceae, Hyaloscyphaceae, Helotiaceae and Lachnaceae.

The diversity of the Leotiomycete a-factor pheromone in the Ploettnerulaceae, Drepanopezizaceae, Mollisiaceae, Erysiphaceae, Hyaloscyphaceae, Helotiaceae and Lachnaceae.</p

Alpha pheromone sequences for all Leotiomycetes (GBK).

Alpha pheromone sequences for all Leotiomycetes (GBK).</p

<i>MAT1-1-13</i> sequences from <i>Rhynchosporium</i> species.

The Leotiomycetes is a hugely diverse group of fungi, accommodating a wide variety of important plant and animal pathogens, ericoid mycorrhizal fungi, as well as producers of antibiotics. Despite their importance, the genetics of these fungi remain relatively understudied, particularly as they don’t include model taxa. For example, sexual reproduction and the genetic mechanisms that underly this process are poorly understood in the Leotiomycetes. We exploited publicly available genomic and transcriptomic resources to identify genes of the mating-type locus and pheromone response pathway in an effort to characterize the mating strategies and behaviors of 124 Leotiomycete species. Our analyses identified a putative a-factor mating pheromone in these species. This significant finding represents the first identification of this gene in Pezizomycotina species outside of the Sordariomycetes. A unique mating strategy was also discovered in Lachnellula species that appear to have lost the need for the primary MAT1-1-1 protein. Ancestral state reconstruction enabled the identification of numerous transitions between homothallism and heterothallism in the Leotiomycetes and suggests a heterothallic ancestor for this group. This comprehensive catalog of mating-related genes from such a large group of fungi provides a rich resource from which in-depth, functional studies can be conducted in these economically and ecologically important species.</div

Alpha pheromone sequences for all Leotiomycetes (FASTA).

Alpha pheromone sequences for all Leotiomycetes (FASTA).</p

Gene alignments and RNAseq mappings of the <i>ste2</i> gene from <i>Hymenoscyphus</i> species.

Gene alignments and RNAseq mappings of the ste2 gene from Hymenoscyphus species.</p

Presence and absence of both pheromone factors and additional functional information of the α-factor pheromone.

Presence and absence of both pheromone factors and additional functional information of the α-factor pheromone.</p