A Venn diagram showing <i>Nasonia vitripennis</i> venom components in other Chalcidoidea species: <i>M</i>. <i>spermotrophus</i>, <i>C</i>. <i>solmsi</i>, <i>T</i>. <i>pretiosum</i> and <i>M</i>. <i>amalphitanum</i>.

A Venn diagram showing Nasonia vitripennis venom components in other Chalcidoidea species: M. spermotrophus, C. solmsi, T. pretiosum and M. amalphitanum.</p

Basic Gene Ontology (GO) analysis terms for <i>M</i>. <i>amalphitanum</i> gene products.

Basic Gene Ontology (GO) analysis terms for M. amalphitanum gene products.</p

Number of homologs of <i>N</i>. <i>vitripennis</i> venom (<i>N</i>. <i>vitripennis</i> toxin constituents) in <i>M</i>. <i>amalphitanum</i> and other Chalcidoidea species based on Universal Chalcidoidea Database [54].

Number of homologs of N. vitripennis venom (N. vitripennis toxin constituents) in M. amalphitanum and other Chalcidoidea species based on Universal Chalcidoidea Database [54].</p

Comparison of TE landscape divergence plots and TE genome fraction pie charts in four parasitoid wasp species: <i>M</i>. <i>amalphitanum</i>, <i>T</i>. <i>pretiosum</i>, <i>N</i>. <i>vitripennis</i> and <i>D</i>. <i>alloeum</i>.

Comparison of TE landscape divergence plots and TE genome fraction pie charts in four parasitoid wasp species: M. amalphitanum, T. pretiosum, N. vitripennis and D. alloeum.</p

Maximum likelihood analysis of phylogenetic relationships between Piwi/Argonaute coding sequences.

Colored dots denote sequences from T. pretiosum (blue), L. clavipes (gray), S. invicta (yellow) and M. amalphitanum (red). Recent duplications in the latter three hymenopterans are indicated by curly brackets, and the corresponding TE divergence plots from [58, 59] and Fig 3 are placed next to each curly bracket. Phylogeny analysis and notations are as in S12 Fig.</p

Final statistics of the genome and transcriptome assemblies of parasitoid wasp <i>Megaphragma amalphitanum</i>.

Final statistics of the genome and transcriptome assemblies of parasitoid wasp Megaphragma amalphitanum.</p

Size comparison of the parasitoid wasp <i>M</i>. <i>amalphitanum</i> and bacterium <i>Thiomargarita namibiensis</i>.

(A) An adult stage of the parasitoid wasp M. amalphitanum (image adapted from [5]), (B) T. namibiensis–the largest known bacterium (modified from Schulz et al. 1999) [11].</p