Kinetic Resolution of Sulfoximines via Asymmetric Organocatalyzed Formation of Benzothiadiazine-1-oxides

A catalytic kinetic resolution of sulfoximines has been developed through chiral phosphoric acid-catalyzed intramolecular dehydrative cyclizations. A variety of racemic sulfoximines bearing an ortho-amidophenyl moiety underwent asymmetric dehydrative cyclizations using this method, yielding both the recovered sulfoximines and benzothiadiazine-1-oxide products with good to high enantioselectivities (with s-factor up to 61). The diverse derivatizations of the chiral products into a wide range of S-stereogenic center-containing S,N-heterocycles have demonstrated the value of this method

Selective Synthesis of Isoquinolines by Rhodium(III)-Catalyzed C–H/N–H Functionalization with α‑Substituted Ketones

A rhodium­(III)-catalyzed C–H/N–H bond functionalization for the synthesis of 1-aminoisoquinolines from aryl amidines and α-MsO/TsO/Cl ketones was achieved under mild reaction conditions. Thus, this approach provides a practical method for the site-selective synthesis of various synthetically valuable isoquinolines with wide functional group tolerance

Carboxylate-Enhanced Rhodium(III)-Catalyzed Aryl C–H Alkylation with Conjugated Alkenes under Mild Conditions

Rhodium­(III)-catalyzed C–H bond functionalization for the synthesis of β-aryl aldehydes and ketones from (hetero)­aryl oximes, pyri­(mi)­dine, as well as pyrazoles and α,β-unsaturated carbonyl compounds has been developed under exceedingly mild reaction conditions. Thus, the versatile rhodium­(III) catalysis features high step- and atom-economy, oxidant-free reaction conditions, and broad substrate scope

Additional file 2: of Blood pressure reduction and clinical outcomes with angiotensin-converting enzyme inhibitors and angiotensin II receptor blockers: protocol for a systematic review and meta-regression analysis

Primary MEDLINE (PubMed) search strategy. (DOCX 14 kb

Amidines for Versatile Cobalt(III)-Catalyzed Synthesis of Isoquinolines through C–H Functionalization with Diazo Compounds

A cobalt­(III)-catalyzed C–H/N–H bond functionalization for the synthesis of 1-aminoisoquinolines from aryl amidines and diazo compounds has been developed. The reaction proceeds under mild reaction conditions, obviates the need for oxidants, produces only N2 and H2O as the byproducts, and features a broad substrate scope

Additional file 1: of Blood pressure reduction and clinical outcomes with angiotensin-converting enzyme inhibitors and angiotensin II receptor blockers: protocol for a systematic review and meta-regression analysis

PRISMA-P 2015 Checklist. (DOCX 30 kb

MOESM3 of Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo

Additional file 3. Effect of NMBRA treatment on the expression of viral NP and cytokines in BMDMs. The BMDMs were mock-treated or treated with NMBRA after infection with PR8 (MOI = 1). NMBRA-treated cells were harvested at 16 hpi. mRNA levels of NP, IFN-α, and IL-6 measured by RT-PCR (A). The expression of NP (B), IFN-α (C), and IL-6 (D) was measured by qRT-PCR. β-Actin was used as the reference housekeeping gene for internal standardization. **P < 0.01

MOESM1 of Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo

Additional file 1. Effect of trypsin on the expression of IL-6 and PAR2 in cells. The 293T and A549 cells were infected with PR8 (MOI = 1) in the presence or absence of trypsin. The 293T cells at 0, 3 and 12 hpi and A549 cells at 0, 3, 12, and 16 hpi were collected to test the expression of IL-6 mRNA in 293T and A549 cells by RT-PCR and qRT-PCR and the expression of PAR2 and NMBR in A549 cells by Western blotting. (A, B) IL-6 mRNA expression in 293T cells. (C) PAR2 expression in 293T cells. (D, E) IL-6 mRNA expression in A549 cells. (F) PAR2 expression in A549 cells. GAPDH and β-actin were used as the reference housekeeping genes for internal standardization

MOESM2 of Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo

Additional file 2. Effect of NMB treatment on the expression of viral NP and cytokines in BMDMs. The BMDMs were mock-treated or treated with NMB after infection with PR8 (MOI = 1). NMB-treated cells were harvested at 16 hpi. The mRNA levels of NP, IFN-α, and IL-6 were measured by RT-PCR (A). qRT-PCR measurement of NP (B), IFN-α (C) and IL-6 mRNA expression (D). β-Actin was used as the reference housekeeping gene for internal standardization. ** P < 0.01

MOESM4 of Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo

Additional file 4. Effect of trypsin on the expression of IL-6, PAR2 and NMBR in vitro. The BMDMs and A549 cells were infected with PR8 (MOI = 1) in the presence or absence of trypsin. The BMDMs and A549 cells were collected at the indicated times to test the expression of IL-6 mRNA by RT-PCR and qRT-PCR and the expression of PAR2 and NMBR by Western blotting. (A, B) IL-6 mRNA expression in BMDMs. (C) PAR2 and NMBR expression in BMDMs. (D) NMBR expression in A549 cells. β-Actin was used as the reference housekeeping gene for internal standardization