Additional file 1 of Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)

Additional file 1

Additional file 9 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 9: Table S6: FPKM expression matrix of PLCP gene, FPKM refers to expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced

Additional file 8 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 8: Table S5: The detailed information of the GO function enrichment list of PLCP gene. (XLS 5 kb

Additional file 3 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 3: Figure S1: Predict the conservative motif of PLCPs by online MEME website

Additional file 2 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 2: Table S2: Protein properties of PLCP gene family in grapevine

Data_Sheet_2_Transcriptional Profiling of Resistant and Susceptible Cultivars of Grapevine (Vitis L.) Reveals Hypersensitive Responses to Plasmopara viticola.pdf

Grapevine downy mildew is the most serious disease of grapevine cultivars that affects the rate of resistance/susceptibility to Plasmopara viticola. In this study, we used the susceptible cultivar “Zitian Seedless” and the resistant cultivar “Kober 5BB” as materials to determine the transcriptome differences and phenotypes of the leaves after inoculation with downy mildew. The differences in microstructures and molecular levels were compared and analyzed. Fluorescence staining and microscopic observations confirmed that hypersensitive cell death occurred around the stomata in “Kober 5BB” infected by downy mildew zoospores. Meanwhile, transcriptomic profiling indicated that there were 11,713 and 6,997 gene expression differences between the resistant and susceptible cultivars at 72 h after inoculation when compared to control (0 h), respectively. The differentially expressed genes of the two cultivars are significantly enriched in different pathways, including response to plant-pathogen interaction, mitogen-activated protein kinase (MAPK) signaling pathway, plant hormone signal transduction, phenylpropanoid, and flavonoid biosynthesis. Furthermore, the results of functional enrichment analysis showed that H2O2 metabolism, cell death, reactive oxygen response, and carbohydrate metabolism are also involved in the defense response of “Kober 5BB,” wherein a total of 322 key genes have been identified. The protein interaction network showed that metacaspases (MCAs), vacuolar processing enzymes (VPEs), and Papain-like cysteine proteases (PLCPs) play an important role in the execution of hypersensitive responses (HR). In conclusion, we demonstrated that HR cell death is the key strategy in the process of grape defense against downy mildew, which may be mediated or activated by Caspase-like proteases.</p

Additional file 4 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 4: Table S3: The Ka/Ks ratios and divergence between paralogous VvPLCP gene pairs

Additional file 11 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 11: Figure S4: Melting curve and amplification curve for qPCR

Additional file 5 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 5: Table S4: Promoter Cis-regulatory elements analysis details of grapevine VvPLCP genes

Additional file 6 of “The PLCP gene family of grapevine (Vitis vinifera L.): characterization and differential expression in response to Plasmopara Viticola”

Additional file 6: Figure S2: Number of each cis-acting element in the promoter region (2.0 kb upstream of the translation start site) of VvPLCP. Based on the functional annotation, the cis-acting elements were classified into three major classes: plant growth and development, phytohormone responsive, or abiotic and biotic stresses-related cis-acting elements genes