Adjusting the Dose of Ag-Ion Implantation on TiN–Ag-Modified SLA-Ti Creates Different Micronanostructures: Implications on Bacteriostasis, Biocompatibility, and Osteogenesis in Dental Implants

The prevention of aseptic loosening and peri-implantitis is crucial for the success of dental implant surgery. In this study, different doses of Ag-implanted TiN/Ag nanomultilayers were prepared on the sandblasting with large grit and acid etching (SLA)-Ti surface using a multiarc ion-plating system and an ion-implantation system, respectively. The physical and chemical properties of the samples were assessed using various techniques, including scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, atomic force microscopy, inductively coupled plasma atomic emission spectrometry, and water contact angle measurements. In addition, the applicability and biosafety of the SLA/1 × 1017-Ag and SLA/1 × 1018-Ag surfaces were determined via biocompatibility testing in vivo and in vitro. The results demonstrated that the physical and chemical properties of SLA/1 × 1017-Ag and SLA/1 × 1018-Ag surfaces were different to some extent. However, compared with SLA-Ti, silver-loaded TiN/Ag-modified SLA-Ti surfaces (SLA/1 × 1018-Ag) with enhanced bacteriostatis, osteogenesis, and biocompatibility have great potential for dental applications

Additional file 2 of High abundance of Ralstonia solanacearum changed tomato rhizosphere microbiome and metabolome

Additional file 2: Table S1. The characters of co-occurrence networks of two groups with different abundance of RS

Additional file 1 of High abundance of Ralstonia solanacearum changed tomato rhizosphere microbiome and metabolome

Additional file 1: Figure S1. Principal coordinates analysis (PCoA) with Bray-Curtis dissimilarity of the rhizosphere bacterial communities using the whole OTU table excluding the OTUs belonging to Ralstonia solanacearum

Additional file 1 of Genome-wide selective signatures mining the candidate genes for egg laying in goose

Supplementary Material 1: Table S1. The statistical table of sequencing results for each sample

Additional file 2: of Root exudates drive the soil-borne legacy of aboveground pathogen infection

R code and root exudate compounds. (ZIP 360 kb

Additional file 1: of Root exudates drive the soil-borne legacy of aboveground pathogen infection

Figure S1. Schematic representation of the experimental design. Figure S2. Detection of Pseudomonas syringae pv. tomato strain DC3000 by PCR. The length of target fragment is 304 bp. Figure S3. Principal coordinate analysis (PCoA) with Bray-Curtis dissimilarity of the microbial community in bulk soil (B) or rhizosphere (R) of control (C) or pathogen-conditioned (P) soils. A) bulk soils, B) rhizosphere soils. Figure S4. A and B are the relative abundance (%) of the bacterial genera within the Firmicutes and Proteobacteria phyla in the microbial communities of control and pathogen- conditioned bulk soils, respectively. C: Relative abundance (%) of the major bacterial phyla using the whole ASV table excluding ten differential ASVs (Fictibacillus and Sphingomonas) present in the microbial communities of control (C) or pathogen-conditioned (P) soils. Samples were taken from the bulk (B) soil or rhizospheres (R) of unchallenged plants. Figure S5. Principal coordinate analysis (PCoA) with Bray-Curtis dissimilarity of the microbial community in bulk soil (B) or rhizosphere (R) of control (C) or pathogen-conditioned (P) soils using the whole ASV table excluding two ASVs belonging to the genera Fictibacillus and Sphingomonas, respectively. a) bulk soils; b) rhizosphere soils; c) all soil samples. Table S1. Soil properties in the control and pathogen-conditioned soil. Table S2. Relative abundance of two highly discriminative ASVs (Fictibacillus and Sphingomonas) in bulk soil samples from the control-conditioned (BC) versus pathogen-conditioned (BP) soils. Table S3. 200–300 ASVs together differentiate the microbial communities of pathogen conditioned and control bulks soil. (DOCX 690 kb