Designing Superlattice Structure via Self-Assembly of One-Component Polymer-Grafted Nanoparticles

The control of the self-assembly of the nanocrystals into ordered structures has been extensively investigated, but fewer efforts have been devoted to studying one-component polymer-grafted nanoparticles (OPNPs). Herein, through coarse-grained molecular dynamics simulation, we design a novel nanoparticle (NP) grafted with polymer chains, focusing on its self-assembled structures. First, we examine the effects of length and density of grafted polymer chains by calculating the radial distribution function between NPs, as well as through direct visualization. We observe a monotonic change of the arranged morphology of grafted-NPs as a function of the density of grafted polymer chains, which indicates that the increase of the grafting density contributes to the order of the morphology. Meanwhile, we find that much longer grafted polymer chains worsen the regularity of the morphology. Then, we probe the influence of the stiffness of grafted polymer chains (denoted by K ranging from 0 to 500) on the order of grafted-NPs, finding that the order of the structure exhibits a nonmonotonic behavior as a function of K at moderate grafting density. For high grafting density, the order of the morphology is initially enhanced and becomes saturated as a function of K. For the effect of K on the stress–strain behavior, the system with the lowest order demonstrates the most remarkable reinforced mechanical behavior for both low and high grafting density. Last, we establish the phase diagram by varying the stiffness and density of the grafted polymer chains, which contains the amorphous, ordered, and superlattice structures, respectively. In general, our simulated results provide guidelines to tailor the self-assembly of the OPNPs by taking advantage of the length, density, and stiffness of grafted polymer chains

DataSheet_1_[18F]AlF-NOTA-ADH-1: A new PET molecular radiotracer for imaging of N-cadherin-positive tumors.docx

BackgroundThe cell adhesion molecule (CAM) N-cadherin has become an important target for tumor therapy. The N-cadherin antagonist, ADH-1, exerts significant antitumor activity against N-cadherin-expressing cancers.MethodsIn this study, [18F]AlF-NOTA-ADH-1 was radiosynthesized. An in vitro cell binding test was performed, and the biodistribution and micro-PET imaging of the probe targeting N-cadherin were also studied in vivo.ResultsRadiolabeling of ADH-1 with [18F]AlF achieved a yield of up to 30% (not decay-corrected) with a radiochemical purity of >97%. The cell uptake study showed that Cy3-ADH-1 binds to SW480 cells but weakly binds to BXPC3 cells in the same concentration range. The biodistribution results demonstrated that [18F]AlF-NOTA-ADH-1 had a good tumor/muscle ratio (8.70±2.68) in patient-derived xenograft (PDX) tumor xenografts but a lower tumor/muscle ratio (1.91±0.69) in SW480 tumor xenografts and lowest tumor/muscle ratio (0.96±0.32) in BXPC3 tumor xenografts at 1 h post-injection (p.i.) These findings were in accordance with the immunohistochemistry results. The micro PET imaging results revealed good [18F]AlF-NOTA-ADH-1 tumor uptake in pancreatic cancer PDX xenografts with strong positive N-calcium expression, while lower tumor uptake in SW480 xenografts with positive expression of N-cadherin, and significantly lower tumor uptake in BXPC3 xenografts with low expression of N-cadherin, which was consistent with the biodistribution and immunohistochemistry results. The N-cadherin-specific binding of [18F]AlF-NOTA-ADH-1 was further verified by a blocking experiment involving coinjection of a non radiolabeled ADH-1 peptide, resulting in a significant reduction in tumor uptake in PDX xenografts and SW480 tumor.Conclusion[18F]AlF-NOTA-ADH-1 was successfully radiosynthesized, and Cy3-ADH-1 showed favorable N-cadherin-specific targeting ability by in vitro data. The biodistribution and microPET imaging of the probe further showed that [18F]AlF-NOTA-ADH-1 could discern different expressions of N-cadherin in tumors. Collectively, the findings demonstrated the potential of [18F]AlF-NOTA-ADH-1 as a PET imaging probe for non-invasive evaluation of the N-cadherin expression in tumors.</p

sj-pdf-1-ajs-10.1177_03635465231208843 – Supplemental material for Fibroblast Activation Protein–Targeted PET/CT with Al18F-NODA-FAPI-04 for In Vivo Imaging of Tendon Healing in Rat Achilles Tendon Injury Models

Supplemental material, sj-pdf-1-ajs-10.1177_03635465231208843 for Fibroblast Activation Protein–Targeted PET/CT with Al18F-NODA-FAPI-04 for In Vivo Imaging of Tendon Healing in Rat Achilles Tendon Injury Models by Zhenfeng Liu, Haiying Zhou, Pengfei Li, Zewei Wang, Tian Tu, Sohaib Hasan Abdullah Ezzi, Vishnu Goutham Kota, Mohamed Hasan Abdulla Hasan Abdulla, Ahmad Alhaskawi, Yanzhao Dong, Yuqiao Huang, Mengjie Dong, Xinhui Su and Hui Lu in The American Journal of Sports Medicine</p