Additional file 1 of Gene profiling of Toll-like receptor signalling pathways in neutrophils of patients with acute-on-chronic liver failure

Additional file 1: Figure S1. Volcano plots showed upregulation and downregulation of marker genes compared to the healthy control group. A. Compensated cirrhosis group compared to the healthy control. B. Decompensated cirrhosis group compared to the healthy control group. C. ACLF group compared to the health control

Clustering analysis of genotyping data of B. cereus clinical endophthalmitis isolates.

<p>Phylogenetic tree of <i>B</i>. <i>cereus</i> clinical endophthalmitis isolates was drawn based on <i>vrrA</i> gene sequence analyses. The figure showed that <i>B</i>. <i>cereus</i> isolats could be grouped into three genotyping (GT) groups: GTI, GTII, and GTIII. GTI was be further divided into three subgroups.</p

Clinical manifestation and prognosis associated with three <i>vrrA</i>-based genotypes of <i>B</i>. <i>cereus</i>.

<p>Clinical manifestation and prognosis associated with three <i>vrrA</i>-based genotypes of <i>B</i>. <i>cereus</i>.</p

B-scan images of the patients' eyes infected with <i>B</i>. <i>cereus</i> before (A,B,C) and after (D,E,F) surgery operation.

<p>(A). Infection caused by GTI strains showed severe vitreous opacity (gay arrows). (B). Infection caused by GTII strains showed mild vitreous opacity (gray arrows). (C). Mild vitreous opacity infection caused by GTIII strains (gray arrow),high reflection and ascoustic shadow in vitreous showed foreign body (white arrow). (D). Infection caused by GTI strains after surgery. (E). Infection caused by GTII strains after surgery. (F). Infection caused by GTIII strains after surgery(the false expansion of eye ball after vitreous surgery with silicon oil tamponade).</p

Patients’ Information.

<p>Patients’ Information.</p

PCR amplification of the <i>vrrA</i> gene among clinical <i>B</i>. <i>cereus</i> isolates.

<p>Lane1-14: Bc1-Bc14; lane16-25: Bc16-Bc25; Lane26: <i>B</i>. <i>cereus</i> ATCC14579; Lane27: <i>B</i>. <i>thuringiensis</i> CTCC22945; Lane28: <i>Bacillus subtilis</i> ATCC9372; Lane29: ddH2O; Lane15 and Lane30: DNA marker. The predicted size of the product was approximately 430bp. A total of twenty–four <i>B</i>. <i>cereus</i> strains were confirmed by the PCR, and non-<i>B</i>. <i>cereus</i> strains did not yield a PCR product.</p

Additional file 1 of Soluble urokinase plasminogen activator receptor is associated with short-term mortality and enhanced reactive oxygen species production in acute-on-chronic liver failure

Additional file 1. Table A. 1. Risk factors for short-term mortality by univariate Cox regression analysis. Figure A. 1. Comparison of plasma suPAR concentrations in different disease groups. No statistical significance was found between each pair of groups. Figure A. 2. The effect of suPAR on ROS production in neutrophils in HC and CHB under E.coli stimulation. Whole blood from healthy controls (n = 13) and patients with CHB (n = 12) was stimulated with E. coli in the presence of suPAR (50 ng/ml) or PBS for 30 minutes in vitro. ROS levels in neutrophils were determined. Statistical analyses were performed using the Wilcoxon signed-rank test. *p < 0.05; ns, not statistically significant. Figure A. 3. The effect of suPAR on ROS production in neutrophils in ACLF without E. coli stimulation. Whole blood from patients with ACLF (n = 10) was incubated with suPAR (50 ng/ml) or PBS for 30 minutes in vitro without E. coli stimulation. ROS levels in neutrophils were determined. Statistical analyses were performed using the Wilcoxon signed-rank test. ns, not statistically significant. Figure A. 4. The correlation of serum suPAR and spontaneous ROS production in neutrophils from ACLF. The spontaneous ROS production in neutrophils from patients with ACLF (n = 11) was directly determined without E.coli stimulation. Statistical analyses were performed using the using spearman correlation. Supplementary methods: The limitations of cytokine measurements. Supplementary methods: Combining MELD or SOFA with suPAR