Identifikasi Bakteri Aeromonas Hydrophila Dengan Uji Mikrobiologi Pada Ikan Lele Dumbo (Clarias Gariepinus) Yang Dibudidayakan Di Kecamatan Baitussalam Kabupaten Aceh Besar

The purpose of this study was to identify and evaluate the prevalence of Aeromonas hydrophila bacteria that attack African catfish to in Aceh Besar district with the microbiological test. This study was conducted on March 2016. Samples were collected from the District recorded have African catfish fish in Aceh Besar district, namely the Baitussalam District. Observations conducted in the laboratory of Bacteria Quarantine Fish Quality Control and Safety of Fishery Class 1 Aceh. This study used a descriptive analytic and the conventional method where the sampling was done by a stratified random sampling method. Further, bacteria was isolated, the morphological of bacteria were observeted, pure cultures included Gram staining, catalase test, test oxidase, biochemical tests include tests Triple Sugar Iron Agar (TSIA), test Lysine Iron Agar (LIA), test citrate, test urea, test Oxidation / fermentative (O/F), Methyl Red test Vogue Proskuer (MRVP), carbohydrate fermentation test, test Motility Indol Ornithine (MIO)and the reading of the identification results were conducted.The result showed that three samples of African catfishfrom concrete pond, were positive infected with 100% prevalance attacked byAeromonas hydrophila. Penelitian ini bertujuan untuk mengidentifikasi serta menghitung nilai prevalensi bakteri Aeromonas hydrophila yang menyerang ikan lele dumbo yang dibudidayakan di Kabupaten Aceh Besar dengan uji mikrobiologi. Penelitian ini dilakukan pada Bulan Maret 2016. Pengambilan sampel dilakukan pada Kecamatan Baitussalam Kabupaten Aceh Besar. Pengamatan bakteri dilakukan di Laboratorium Bakteri Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Kelas 1 Aceh. Penelitian ini menggunakan deskriptif analitik dan metode konvensional dimana pengambilan sampel dilakukan dengan metode pengambilan acak terstratifikasi. Selanjutnya dilakukan isolasi bakteri, pengamatan morfologi, kultur murni meliputi uji Gram, uji katalase, uji oksidase, uji biokimia meliputi uji Triple Sugar Iron Agar (TSIA), uji Lysine Iron Agar (LIA), uji sitrat, uji urea, uji Oksidasi/Fermentatif (O/F), uji Methyl Red Vogue Proskuer (MRVP), uji fermentasi karbohidrat, uji Motility Indol Ornithin (MIO), dan pembacaan hasil identifikasi. Hasil penelitian menunjukkan bahwa 3 sampel ikan lele dumbo dari kolam beton positif terinfeksi bakteri Aeromonas hydrophila dengan prevalensi yang menyerang ikan lele dumbo kolam beton 100%

Pengaruh Media Kultur Yang Berbeda Terhadap Laju Pertumbuhan Dan Biomassa Spirulina SP.

The objective of this study was to determine the effect of the different manures as culture media on the growth and biomass Spirulina sp. This research was conducted from March to May 2016. This research used the one factorial Completely Randomized Design (CRD) as method with four treatments and five replications which is A = 100 g/l of chicken manure, B = 100 g/l of bird manure, C = 100 g/l of buffalo manure and D = 100 g/l of cow manure. The result of Analysis of Varians (ANOVA) showed that the different manures as culture media gave the effect (P<0,05) on the growth and biomass of Spirulina sp. The best treatment was obtained at treatment B (chicken manure) with the average of population density was 7,47 x 106 ind/ml and the average of biomass weight was 0,0506