Patients’ Information.

<p>Patients’ Information.</p

PCR amplification of the <i>vrrA</i> gene among clinical <i>B</i>. <i>cereus</i> isolates.

<p>Lane1-14: Bc1-Bc14; lane16-25: Bc16-Bc25; Lane26: <i>B</i>. <i>cereus</i> ATCC14579; Lane27: <i>B</i>. <i>thuringiensis</i> CTCC22945; Lane28: <i>Bacillus subtilis</i> ATCC9372; Lane29: ddH2O; Lane15 and Lane30: DNA marker. The predicted size of the product was approximately 430bp. A total of twenty–four <i>B</i>. <i>cereus</i> strains were confirmed by the PCR, and non-<i>B</i>. <i>cereus</i> strains did not yield a PCR product.</p

Clinical manifestation and prognosis associated with three <i>vrrA</i>-based genotypes of <i>B</i>. <i>cereus</i>.

<p>Clinical manifestation and prognosis associated with three <i>vrrA</i>-based genotypes of <i>B</i>. <i>cereus</i>.</p

Clustering analysis of genotyping data of B. cereus clinical endophthalmitis isolates.

<p>Phylogenetic tree of <i>B</i>. <i>cereus</i> clinical endophthalmitis isolates was drawn based on <i>vrrA</i> gene sequence analyses. The figure showed that <i>B</i>. <i>cereus</i> isolats could be grouped into three genotyping (GT) groups: GTI, GTII, and GTIII. GTI was be further divided into three subgroups.</p

B-scan images of the patients' eyes infected with <i>B</i>. <i>cereus</i> before (A,B,C) and after (D,E,F) surgery operation.

<p>(A). Infection caused by GTI strains showed severe vitreous opacity (gay arrows). (B). Infection caused by GTII strains showed mild vitreous opacity (gray arrows). (C). Mild vitreous opacity infection caused by GTIII strains (gray arrow),high reflection and ascoustic shadow in vitreous showed foreign body (white arrow). (D). Infection caused by GTI strains after surgery. (E). Infection caused by GTII strains after surgery. (F). Infection caused by GTIII strains after surgery(the false expansion of eye ball after vitreous surgery with silicon oil tamponade).</p

Photographs

Photograph

Prevalence of Human Parvovirus B19, Bocavirus, and PARV4 in Blood Samples from the General Population of China and Lack of a Correlation between Parvovirus and Hepatitis B Co-Infection

<div><p>Few comprehensive studies have investigated viraemia caused by human parvoviruses (HPAVs) in China. A total of 1626 of blood samples were collected from non-HBV and HBV infected Chinese subjects (adults, N = 1279; children, N = 347) from south-western and south-eastern China. DNA from three HPAVs was detected in blood samples using PCR-based assays. The epidemiological profiles and association with HBV co-infection were also analysed. Of the 1626 blood samples tested, 138 (8.49%) were found to exhibit HPAV viraemia, including 3.51% with B19, 3.75% with HBoV and 2.52% with PARV4. The presence of B19 DNA in both child and adult, as well as that of PARV4 DNA in adult,from the south-western region was significantly higher than that from the south-eastern region (<i>P</i> = 0.006 for B19 in children; <i>P</i> = 0.026 for B19 in adults; and <i>P</i> = 0.014 for PARV4 in adult).However, the frequency of HBoV DNA in adults from the south-western region was significantly lower than that observed in adults from the south-eastern region (<i>P = </i>0.001). Furthermore, HBoV was more prevalence in male (4.9%) than in female (1.4%) individuals. In addition, no significant correlation between HBV and HPAV co-infection was found using serum samples from Chinese adults. In conclusions,the molecular prevalence of three HPAVs in blood samples exhibited variation among different populations depending on area, age and gender; No association between HPAV and HBV infection in adults was found. Our data provide a basis for improving blood safety and preventing HPAV infection in China.</p></div

Location of samples collected for screening of three human parvoviruses (HPAVs) in China.

<p>Location of samples collected for screening of three human parvoviruses (HPAVs) in China.</p

Demographic characteristics of all participants (N = 1626).

<p>Demographic characteristics of all participants (N = 1626).</p

Prevalences of B19, HBoV and PARV4 in Han and Tibetan populations in the south-western region.

<p>Prevalences of B19, HBoV and PARV4 in Han and Tibetan populations in the south-western region.</p