Basic characteristics of the individuals at presentation.

*<p>Migrants: people who reside Shenzhen but keep “<i>hukou</i>” in their hometown <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067516#pone.0067516-Jia1" target="_blank">[12]</a>.</p

A. The trend of TB notification rate during 1993–2010.

<p>B. Overall treatment delay trend over time stratified from 1993–2010. C. Proportion of different treatment delay durations over time from 1993–2010.</p

Risk factors related with treatment delay.

<p>Risk factors related with treatment delay.</p

Effect of delay on transmission of tuberculosis.

<p>Effect of delay on transmission of tuberculosis.</p

I-309, IL-8 and MIG over-expression is stimulated by Mtb antigens (ESAT-6 and CFP-10) in active TB.

<p>Expression of I-309 (A), IL-8 (B), and MIG (C) induced by PBS or specific TB antigens in whole blood displayed a gradual increase from health controls (n = 80) to individuals with LTBI (n = 45), to those with active TB (n = 80). Mean values ± standard error are shown, *P < 0.05; **P < 0.01 (Student’s <i>t</i>-test).</p

The arrangement of cytokines and antigens on TB-relate cytokine and TB-specific antigen array.

<p>Mouse anti-humane-IgG IgG was used as positive control and BSA were used as negative control.</p

Up-regulation of I-309, IL-8, and MIG in the serum of active TB patients.

<p>(A-C) quantitative humane cytokine array analysis of peripheral serum samples from health controls (n = 20), individuals with LTBI (n = 20) and patients with active TB (n = 20). Blue, I-309; red, IL-8; yellow, MIG. (D-F) ELISA analysis for the confirmation of I-309, IL-8 and MIG expression in peripheral serum samples from health controls, patients with active TB, and individuals with LTBI or other pulmonary diseases (n = 45 in each group). Mean values ± standard error are shown, * P<0.05; ** P < 0.01 (Student’s <i>t</i>-test).</p

Determination of cut-off values for I-309, IL-8 and MIG in the diagnosis of active TB.

<p>Determination of cut-off values for I-309, IL-8 and MIG in the diagnosis of active TB.</p