A study of the reactions of third grade pupils to the various types of children's literature that were available during three specific periods: 1900-1920; 1920-1940; 1940-1953

Thesis (Ed.M.)--Boston Universit

What is the most effective way to treat recurrent canker sores?

Amlexanox appears to be most effective overall. Amlexanox 5% paste reduces ulcer size, pain duration, and healing time (strength of recommendation [SOR]: A, multiple randomized controlled trials [RCTs]). Topical steroids may alleviate pain and decrease ulcer burden, defined as total number of ulcers over a measured time, usually 4 to 6 weeks (SOR: B, multiple small heterogenous RCTs). Chemical cautery agents also lessen pain (SOR: A, single RCTs on differing agents), and chlorhexidine mouthwashes may reduce overall ulcer burden (SOR: B, heterogeneous RCTs). The herbal preparation Eupatorium laevigatum alleviates pain and improves healing (SOR: B, single RCT). Oral vitamin B12 supplements and avoiding toothpastes containing sodium lauryl sulfate may prevent recurrent ulcers (SOR: B, small RCTs)

Extragalactic Constraints on the Initial Mass Function

The local stellar mass density is observed to be significantly lower than the value obtained from integrating the cosmic star formation history (SFH), assuming that all the stars formed with a Salpeter initial mass function (IMF). Even other favoured IMFs, more successful in reconciling the observed $z=0$ stellar mass density with that inferred from the SFH, have difficulties in reproducing the stellar mass density observed at higher redshift. In this study we investigate to what extent this discrepancy can be alleviated for any universal power-law IMF. We find that an IMF with a high-mass slope shallower (2.15) than the Salpeter slope (2.35) reconciles the observed stellar mass density with the cosmic star formation history, but only at low redshifts. At higher redshifts $z>0.5$ we find that observed stellar mass densities are systematically lower than predicted from the cosmic star formation history, for any universal power-law IMF.Comment: 6 pages, 5 figures, accepted to MNRA

Photometric Catalogue of Quasars and Other Point Sources in the Sloan Digital Sky Survey

We present a catalogue of about 6 million unresolved photometric detections in the Sloan Digital Sky Survey Seventh Data Release classifying them into stars, galaxies and quasars. We use a machine learning classifier trained on a subset of spectroscopically confirmed objects from 14th to 22nd magnitude in the SDSS {\it i}-band. Our catalogue consists of 2,430,625 quasars, 3,544,036 stars and 63,586 unresolved galaxies from 14th to 24th magnitude in the SDSS {\it i}-band. Our algorithm recovers 99.96% of spectroscopically confirmed quasars and 99.51% of stars to i $\sim$21.3 in the colour window that we study. The level of contamination due to data artefacts for objects beyond $i=21.3$ is highly uncertain and all mention of completeness and contamination in the paper are valid only for objects brighter than this magnitude. However, a comparison of the predicted number of quasars with the theoretical number counts shows reasonable agreement.Comment: 16 pages, Ref. No. MN-10-2382-MJ.R2, accepted for publication in MNRAS Main Journal, April 201

Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria

peer-reviewedAbstract A bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s responsible for driving the changes in the intestinal cells. The adherence of various bifidobacteria to HT-29 cells was increased when the intestinal cells were pre-incubated with immunoglobulin G (IgG). Modulation of the intestinal cells by IgG was concentration dependent with 16 mg/mL IgG resulting in a 43-fold increase in the adhesion of Bifidobacterium longum NCIMB 8809 to HT-29 cells. Periodate treatment of colostral IgG prior to performing the colonization studies resulted in a reduction in the adhesion of the strain to the intestinal cells demonstrating that the glycans of IgG may be important in modulating the intestinal cells for enhanced commensal adhesion. IgG isolated from mature milk also resulted in significant increases in adhesion of the Bifidobacterium strains tested albeit at reduced levels (3.9-fold). The impact of IgG on the HT-29 cells was also visualised via scanning electron microscopy. This study builds a strong case for the inclusion of IgG ingredients sourced from cow’s milk in functional foods aimed at increasing numbers of health promoting bacteria in the human gut

Characterisation of nerve‐mediated ATP release from bladder detrusor muscle and its pathological implications

Background and Purpose. To characterise the molecular mechanisms that determine variability of atropine‐resistance of nerve‐mediated contractions in human and guinea‐pig detrusor smooth muscle Experimental Approach. Atropine‐resistance of nerve‐mediated contractions, and the role of P2X1 receptors, was measured in isolated preparations from guinea‐pigs and also humans with or without overactive bladder syndrome, from which the mucosa was removed. Nerve‐mediated ATP release was measured directly with amperometric ATP‐sensitive electrodes. Ecto‐ATPase activity of guinea‐pig and human detrusor samples was measured in vitro by measuring the concentration‐dependent rate of ATP breakdown. The transcription of ecto‐ATPase subtypes in human samples was measured by qPCR. Key Results Atropine resistance was greatest in guinea‐pig detrusor, absent in human tissue from normally‐functioning bladders and intermediate in human overactive bladder. Greater atropine resistance correlated with reduction of contractions by the ATP‐diphospho‐hydrolase apyrase, directly implicating ATP in their generation. E‐NTPDase‐1 was the most abundantly transcribed ecto‐ATPase of those tested and transcription was reduced in tissue from human overactive, compared to normal, bladders. E‐NTPDase‐1 enzymatic activity was inversely related to the magnitude of atropine resistance. Nerve‐mediated ATP release was continually measured and varied with stimulation frequency over the range 1‐16 Hz. Conclusion and Implications Atropine‐resistance in nerve‐mediated detrusor contractions is due to ATP release and its magnitude is inversely related to E‐NTPDase‐1 activity. ATP is released under different stimulation conditions compared to acetylcholine that implies different routes for their release</p

Radio Emission and AGN Feedback in Post-starburst Galaxies

We investigate radio-mode AGN activity among post-starburst galaxies from the Sloan Digital Sky Survey to determine whether AGN feedback may be responsible for the cessation of star formation. Based on radio morphology and radio-loudness from the FIRST and NVSS data, we separate objects with radio activity due to an AGN from ongoing residual star formation. Of 513 SDSS galaxies with strong A-star spectra, 12 objects have 21-cm flux density above 1 mJy. These galaxies do not show optical AGN emission lines. Considering that the lifetime of radio emission is much shorter than the typical time-scale of the spectroscopic features of post-starburst galaxies, we conclude that the radio-emitting AGN activity in these objects was triggered after the end of the recent starburst, and thus cannot be an important feedback process to explain the post-starburst phase. The radio luminosities show a positive correlation with total galaxy stellar mass, but not with the mass of recently formed stars. Thus the mechanical power of AGN feedback derived from the radio luminosity is related to old stellar populations dominating the stellar mass, which in turn are related to the masses of central supermassive black holes.Comment: 11 pages, 6 figures, accepted for publication in MNRA

Multi-parallel qPCR provides increased sensitivity and diagnostic breadth for gastrointestinal parasites of humans: field-based inferences on the impact of mass deworming

BACKGROUND: Although chronic morbidity in humans from soil transmitted helminth (STH) infections can be reduced by anthelmintic treatment, inconsistent diagnostic tools make it difficult to reliably measure the impact of deworming programs and often miss light helminth infections. METHODS: Cryopreserved stool samples from 796 people (aged 2-81 years) in four villages in Bungoma County, western Kenya, were assessed using multi-parallel qPCR for 8 parasites and compared to point-of-contact assessments of the same stools by the 2-stool 2-slide Kato-Katz (KK) method. All subjects were treated with albendazole and all Ascaris lumbricoides expelled post-treatment were collected. Three months later, samples from 633 of these people were re-assessed by both qPCR and KK, re-treated with albendazole and the expelled worms collected. RESULTS: Baseline prevalence by qPCR (n = 796) was 17 % for A. lumbricoides, 18 % for Necator americanus, 41 % for Giardia lamblia and 15% for Entamoeba histolytica. The prevalence was <1% for Trichuris trichiura, Ancylostoma duodenale, Strongyloides stercoralis and Cryptosporidium parvum. The sensitivity of qPCR was 98% for A. lumbricoides and N. americanus, whereas KK sensitivity was 70% and 32%, respectively. Furthermore, qPCR detected infections with T. trichiura and S. stercoralis that were missed by KK, and infections with G. lamblia and E. histolytica that cannot be detected by KK. Infection intensities measured by qPCR and by KK were correlated for A. lumbricoides (r = 0.83, p < 0.0001) and N. americanus (r = 0.55, p < 0.0001). The number of A. lumbricoides worms expelled was correlated (p < 0.0001) with both the KK (r = 0.63) and qPCR intensity measurements (r = 0.60). CONCLUSIONS: KK may be an inadequate tool for stool-based surveillance in areas where hookworm or Strongyloides are common or where intensity of helminth infection is low after repeated rounds of chemotherapy. Because deworming programs need to distinguish between populations where parasitic infection is controlled and those where further treatment is required, multi-parallel qPCR (or similar high throughput molecular diagnostics) may provide new and important diagnostic information

Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses

Plant plasma membrane localized pattern recognition receptors (PRRs) detect extracellular pathogen-associated molecules. PRRs such as Arabidopsis EFR and rice XA21 are taxonomically restricted and are absent from most plant genomes. Here we show that rice plants expressing EFR or the chimeric receptor EFR::XA21, containing the EFR ectodomain and the XA21 intracellular domain, sense both Escherichia coli- and Xanthomonas oryzae pv. oryzae (Xoo)-derived elf18 peptides at sub-nanomolar concentrations. Treatment of EFR and EFR::XA21 rice leaf tissue with elf18 leads to MAP kinase activation, reactive oxygen production and defense gene expression. Although expression of EFR does not lead to robust enhanced resistance to fully virulent Xoo isolates, it does lead to quantitatively enhanced resistance to weakly virulent Xoo isolates. EFR interacts with OsSERK2 and the XA21 binding protein 24 (XB24), two key components of the rice XA21-mediated immune response. Rice-EFR plants silenced for OsSERK2, or overexpressing rice XB24 are compromised in elf18-induced reactive oxygen production and defense gene expression indicating that these proteins are also important for EFR-mediated signaling in transgenic rice. Taken together, our results demonstrate the potential feasibility of enhancing disease resistance in rice and possibly other monocotyledonous crop species by expression of dicotyledonous PRRs. Our results also suggest that Arabidopsis EFR utilizes at least a subset of the known endogenous rice XA21 signaling components