Physical parameters, modeling, and methodological details in using IR laser pulses to warm frozen or vitrified cells ultra-rapidly

We report additional details of the thermal modeling, selection of the laser, and construction of the Cryo Jig used for our ultra-rapid warming studies of mouse oocytes (Jin et al., 2014). A Nd:YAG laser operating at 1064 nm was selected to deliver short 1ms pulses of sufficient power to produce a warming rate of 1×10(7)°C/min from -190°C to 0°C. A special Cryo Jig was designed and built to rapidly remove the sample from LN2 and expose it to the laser pulse. India ink carbon black particles were required to increase the laser energy absorption of the sample. The thermal model reported here is more general than that previously reported. The modeling reveals that the maximum warming rate achievable via external warming across the cell membrane is proportional to (1/R(2)) where R is the cell radius

Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse

Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that they be cooled at very high rates to below − 100°C. We report here that neither of these beliefs holds for mouse oocytes. Rather, we find that if mouse oocytes are suspended in media that produce considerable osmotic dehydration before vitrification and are subsequently warmed at ultra high rates (10,000,000°C/min) achieved by a laser pulse, nearly 100% will survive even when cooled rather slowly and when the concentration of solutes in the medium is only 1/3rd of standard

Phase-coherent lightwave communications with frequency combs

Fiber-optical networks are a crucial telecommunication infrastructure in society. Wavelength division multiplexing allows for transmitting parallel data streams over the fiber bandwidth, and coherent detection enables the use of sophisticated modulation formats and electronic compensation of signal impairments. In the future, optical frequency combs may replace multiple lasers used for the different wavelength channels. We demonstrate two novel signal processing schemes that take advantage of the broadband phase coherence of optical frequency combs. This approach allows for a more efficient estimation and compensation of optical phase noise in coherent communication systems, which can significantly simplify the signal processing or increase the transmission performance. With further advances in space division multiplexing and chip-scale frequency comb sources, these findings pave the way for compact energy-efficient optical transceivers.Comment: 17 pages, 9 figure

On Mathematics, Imagination and the Beauty of Numbers

Mathematic

Personality profiles of young chess players

Although the game of chess has often featured in psychological research, we know very little about people who play chess, especially about children who take up chess as a hobby. This study presents the personality profiles as measured with the Big Five model (BFQ-C; Barbaranelli et al., 2003) of 219 young children who play chess and 50 of their peers who do not. Children who score higher on Intellect/openness and Energy/extraversion are more likely to play chess while children who score higher on Agreeableness are less likely to be attracted to chess. Boys with higher scores on Agreeableness are less likely to take up chess than boys with lower scores. Considering that girls score higher on Agreeableness, this factor may provide one of the possible reasons why more boys are interested in chess. Although none of the Big Five factors were associated with self-reported skill level, a sub-sample of 25 elite players had significantly higher scores on Intellect/openness than their weaker chess playing peers

High spectral efficiency superchannel transmission using a soliton microcomb

Optical communication systems have come through five orders of magnitude improvement in data rate over the last three decades. The increased demand in data traffic and the limited optoelectronic component bandwidths have led to state-of-the-art systems employing hundreds of separate lasers in each transmitter. Given the limited optical amplifier bandwidths, focus is now shifting to maximize the spectral efficiency, SE. However, the frequency jitter from neighbouring lasers results in uncertainties of the exact channel wavelength, requiring large guardbands to avoid catastrophic channel overlap. Optical frequency combs with optimal line spacings (typically around 10-50 GHz) can overcome these limitations and maximize the SE. Recent developments in microresonator-based soliton frequency combs (hereafter microcombs) promise a compact, power efficient multi-wavelength and phase-locked light source for optical communications. Here we demonstrate a microcomb-based communication link achieving state-of-the-art spectral efficiency that has previously only been possible with bulk-optics systems. Compared to previous microcomb works in optical communications, our microcomb features a narrow line spacing of 22.1 GHz. In addition, it provides a four order-of-magnitude more stable line spacing compared to free-running lasers. The optical signal-to-noise ratio (OSNR) is sufficient for information encoding using state-of-the-art high-order modulation formats. This enables us to demonstrate transmission of a 12 Tb/s superchannel over distances ranging from a single 82 km span with an SE exceeding 10 bits/s/Hz, to 2000 km with an SE higher than 6 bits/s/Hz. These results demonstrate that microcombs can attain the SE that will spearhead future optical networks

International cooperation for Mars exploration and sample return

The National Research Council's Space Studies Board has previously recommended that the next major phase of Mars exploration for the United States involve detailed in situ investigations of the surface of Mars and the return to earth for laboratory analysis of selected Martian surface samples. More recently, the European space science community has expressed general interest in the concept of cooperative Mars exploration and sample return. The USSR has now announced plans for a program of Mars exploration incorporating international cooperation. If the opportunity becomes available to participate in Mars exploration, interest is likely to emerge on the part of a number of other countries, such as Japan and Canada. The Space Studies Board's Committee on Cooperative Mars Exploration and Sample Return was asked by the National Aeronautics and Space Administration (NASA) to examine and report on the question of how Mars sample return missions might best be structured for effective implementation by NASA along with international partners. The committee examined alternatives ranging from scientific missions in which the United States would take a substantial lead, with international participation playing only an ancillary role, to missions in which international cooperation would be a basic part of the approach, with the international partners taking on comparably large mission responsibilities. On the basis of scientific strategies developed earlier by the Space Studies Board, the committee considered the scientific and technical basis of such collaboration and the most mutually beneficial arrangements for constructing successful cooperative missions, particularly with the USSR

Extrinsic Curvature Dependence of Nielsen-Olesen Strings

It is shown how to treat the degrees of freedom of Nielsen-Olesen vortices in the $3+1$-dimensional $U(1)$ higgs model by a collective coordinate method. In the london limit, where the higgs mass becomes infinite, the gauge and goldstone degrees of freedom are integrated out, resulting in the vortex world-sheet action. Introducing an ultraviolet cut-off mimics the effect of finite higgs mass. This action is non-polynomial in derivatives and depends on the extrinsic curvature of the surface. Flat surfaces are stable if the coherence length is less than the penetration depth. It is argued that in the quantum abelian higgs model, vortex world-sheets are dominated by branched polymers.Comment: 12 pages, latex, CCNY-HEP-94-3 Some points in the text are clarified and new references are include

An integrative approach unveils FOSL1 as an oncogene vulnerability in KRAS-driven lung and pancreatic cancer

KRAS mutated tumours represent a large fraction of human cancers, but the vast majority remains refractory to current clinical therapies. Thus, a deeper understanding of the molecular mechanisms triggered by KRAS oncogene may yield alternative therapeutic strategies. Here we report the identification of a common transcriptional signature across mutant KRAS cancers of distinct tissue origin that includes the transcription factor FOSL1. High FOSL1 expression identifies mutant KRAS lung and pancreatic cancer patients with the worst survival outcome. Furthermore, FOSL1 genetic inhibition is detrimental to both KRAS-driven tumour types. Mechanistically, FOSL1 links the KRAS oncogene to components of the mitotic machinery, a pathway previously postulated to function orthogonally to oncogenic KRAS. FOSL1 targets include AURKA, whose inhibition impairs viability of mutant KRAS cells. Lastly, combination of AURKA and MEK inhibitors induces a deleterious effect on mutant KRAS cells. Our findings unveil KRAS downstream effectors that provide opportunities to treat KRAS-driven cancers