7 research outputs found
Effect of plasmin on neutrophil activation.
<p>Fluorescence histograms for expression of CD11b/Mac-1 and CD62L/L-selectin on murine neutrophils undergoing stimulation with plasmin, PMA (open histograms, broken lines), or vehicle (open histograms, solid lines) as compared to control IgG (solid histograms).</p
Role of mast cells and lipid mediators for plasmin-dependent leukocyte responses.
<p>The number of ruthenium red-positive cells in the cremaster muscle of mice treated with cromolyn, MK-886, or BN52021 undergoing stimulation with plasmin (A). Leukocyte responses in the cremaster muscle of mast cell-depleted mice as well as of mice treated with cromolyn (B, D), MK-886, BN52021 (C, E), or vehicle undergoing stimulation with plasmin (mean±SEM for <i>n</i> = 4–6 per group; #p<0.05, vs. unstimulated; *p<0.05, vs. vehicle; §p<0.05, vs. MK-886; °p<0.05, vs. cromolyn).</p
Systemic leukocyte counts as well as microhemodynamic parameters, including inner vessel diameter, blood flow velocity, and wall shear rate were obtained as detailed in <i>Material and Methods</i> (mean±SEM for <i>n</i> = 3–6 per group).
<p>Systemic leukocyte counts as well as microhemodynamic parameters, including inner vessel diameter, blood flow velocity, and wall shear rate were obtained as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017229#s4" target="_blank"><i>Material and Methods</i></a> (mean±SEM for <i>n</i> = 3–6 per group).</p
Effect of plasmin on leukocyte rolling, firm adherence, and transmigration.
<p>Leukocyte responses in the cremaster muscle after intraarterial (A, C, E) or intrascrotal (B, D, F) injection of plasmin (mean±SEM for <i>n</i> = 3 per group; *p<0.05, vs. control).</p
Effect of TXA, EACA, and aprotinin on postischemic leukocyte responses.
<p>Postcapillary venules in the postischemic cremaster muscle treated with ε-aminocaproic acid (EACA) or vehicle (A; scale bar 25 µm). Leukocyte rolling (B), firm adherence (C), and transmigration (D) in mice treated with tranexamic acid (TXA), EACA, or aprotinin undergoing I/R (mean±SEM; n = 6 per group; #p<0.05, vs. sham; *p<0.05, vs. vehicle).</p
Effect of TXA, EACA, and aprotinin on postischemic remodeling of the perivenular basement membrane.
<p>Postcapillary venule in the postischemic cremaster muscle immunostained for collagen IV. White rings show low-expression regions (LER; A; scale bar 10 µm). Size (B) and density (C) of LER in mice treated with TXA, EACA, or aprotinin undergoing I/R (mean±SEM; <i>n</i> = 4 per group; #<i>p</i><0.05, vs. unstimulated; *<i>p</i><0.05, vs. vehicle).</p
Role of mast cells and lipid mediators for postischemic leukocyte responses.
<p>Cremasteric ruthenium-red-positive mast cells (A; arrows). The number of ruthenium red-positive cells in mice treated with TXA, EACA, aprotinin, cromolyn, MK-886, or BN52021 undergoing I/R (B). Leukocyte firm adherence (C) and transmigration (D) in mast cell-depleted mice as well as in mice treated with cromolyn, BN52021, or MK-886 undergoing I/R (mean±SEM for <i>n</i> = 4–5 per group; #p<0.05, vs. sham; *p<0.05, vs. vehicle).</p