Additional file 2: Table S2. of Dynamics of sterol synthesis during development of Leishmania spp. parasites to their virulent form

Homologous genes of Leishmania spp. sterol biosynthetic pathway (SBP). Trypanosoma cruzi SBP genes were used as gene ID during database search except those marked with an “*”, in which keyword search was used. Blank cells indicate data not available. (XLSX 17 kb

Additional file 1: Table S1. of Dynamics of sterol synthesis during development of Leishmania spp. parasites to their virulent form

Effect of different culture media on absolute sterol content of stationary-phase promastigotes of a virulent strain compared to an avirulent strain of Leishmania infantum +. (DOCX 13 kb

Additional file 1: of Extracellular release of virulence factor major surface protease via exosomes in Leishmania infantum promastigotes

Figure S1. Highlight of the peptides and residues identified as indicators of protein class: stationary (S), logarithmic (L), or constitutive (C). The small number after each amino acid section shows the location in the protein sequence. N and C indicate the N-terminal and the C-terminal of the proteins, respectively. See Table 1 for accession numbers. (DOCX 312 kb

Additional file 2: of Extracellular release of virulence factor major surface protease via exosomes in Leishmania infantum promastigotes

Figure S2. Alignment of all 17 proteins identified. The first four proteins are examples of previously identified S, L, and two C class proteins with accession numbers: LcMSPS1 (Yao 2003) = M80669, LcMSPL1 (Yao et al. [24]) = M80672, LEIDOC1 (Ramamoorthy et al. [8]) = AAA29237.1, MSPC_partial = CAC37969. Accession numbers of the remaining 17 proteins are provided in Table 1. Underlined proteins are identical to already classified proteins: GP63_LEIDO(S) = ‘MSPS4’ in Yao et al. [24]; LcMSPL1(L) = ‘MSPL1’ on NCBI (accession number M80672); GP63_LEIDO6(C) = ‘MSPC’ on NCBI (accession number CAC37953); GP63LEIDO5(C) = ‘constitutive major surface protease’ on NCBI (accession number CAC37955); GP63_LEIME(C) = ‘GP63-C1’ on NCBI (accession number P43150); GP63_LEITR(C) = ‘MSPC’ in Yao et al. [24]. Underlined italics indicate the peptides that were identified by LC-MS/MS. Blue highlight indicates how MSPL1 is differentiated from MSPS1 (Roberts et al. [30]); yellow highlight: suggestive of a C class; black highlight: key features of an S class as described in Roberts et al. [9, 30]; grey highlight: areas that show ambiguity towards the classification (due to being a key feature of another class or a non-common feature); light blue highlight: anchor addition site. (DOCX 34 kb

Supplementary Tables 1-5 from Biomarkers of Dietary Energy Restriction in Women at Increased Risk of Breast Cancer

Supplementary Tables 1-5 from Biomarkers of Dietary Energy Restriction in Women at Increased Risk of Breast Cance

Appendix from Breast Cancer Risk in Young Women in the National Breast Screening Programme: Implications for Applying NICE Guidelines for Additional Screening and Chemoprevention

NICE guidelines.</p

Supplementary Figure 1 from Breast Cancer Risk in Young Women in the National Breast Screening Programme: Implications for Applying NICE Guidelines for Additional Screening and Chemoprevention

Sensitivity analysis.</p

The Effect of 1,3-Diaryl-[1H]-pyrazole-4-acetamides on Glucose Utilization in ob/ob Mice

This article provides evidence of a new class of compounds, 1,3-diaryl-[1H]-pyrazole-4-acetamides, initially identified from their ability to increase glucose transport in an adipocyte and muscle cell line and ultimately demonstrating dramatic glucose lowering in ob/ob mice, a diabetic animal model. The lead compound, 1, possessed some behavioral-like effects which were removed by structural variation during the course of this investigation. Specifically, 11g (R1 = meta-CF3, Ar2 = 4‘biphenyl, R3 = diethylamide) illustrated the potency of this series with ED50 values for glucose lowering in ob/ob mice of 3.0 mg/kg/day. Concomitant with its effect on glucose lowering, 11g also caused a 50% reduction in insulin levels consistent with an agent that increases whole body insulin sensitivity. 11g showed favorable pharmacokinetic data with acceptable absorption, negligible metabolism, and good duration of action. 11g demonstrated no appreciable adipogenic effect through PPARγ agonism, a characteristic of the thiazolidinediones (TZD), and so represents a potentially new class of agents for the treatment of diabetes

Additional file 1: Appendix 1. of Mammographic density adds accuracy to both the Tyrer-Cuzick and Gail breast cancer risk models in a prospective UK screening cohort

Density residual: description of methods. Appendix 2: Table S1. Univariate and multivariate performance of breast density and the Tyrer-Cuzick and Gail risk models, subgroup analysis by time of cancer diagnosis. (PDF 396 kb