Appendix B. Detailed collection information about the morphospecies.

Detailed collection information about the morphospecies

Appendix C. A table listing the 102 observed together with their occurrence patterns and observations history.

A table listing the 102 observed together with their occurrence patterns and observations history

Appendix D. Tabular results from primary and supplementary statistical tests.

Tabular results from primary and supplementary statistical tests

A Statistical Evaluation of Methods of In-Vitro Growth Assessment for <i>Phyllosticta citricarpa</i>: Average Colony Diameter <i>vs</i>. Area

<div><p>Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (D_A) and the measured area (M_A) for assessing fungicide growth inhibition of the ascomycete, <i>Phyllosticta citricarpa</i> on solid media. Both the calculated, D_A and the actual measured area, M_A were adequate for distinguishing significant treatment effects of fungicide on fungal growth, however M_A was more sensitive at identifying significant differences between the controls and fungicide concentrations below 5 ppm.</p></div

Area of fungal growth measured, M_A (dark bar) or calculated, D_A (light bar) from average diameter for Days 7, 14 and 21 for each fungicide level tested.

<p>The error-bars are the 95% confidence intervals for each mean.</p

Day 7 growth of <i>Phyllosticta citricarpa</i> on unamended PDA.

<p>(A) Measurements D1 and D2 are the perpendicular length for estimating growth and used to compute calculated area (D_A). The red circle is the approximate placement and size of the plug. (B) Measured area (M_A) is represented by the area encompassed by the white outline of the colony.</p

Comparison of LS-Means for the three-way interaction, Media*Day*Method, where dark bars represent measured Area (M_A) and light bars represent area calculated from Diameter (D_A).

<p>A. Inhibition ratios calculated using un-amended PDA as control (Ratio₀) for each time point, Days 7, 14 and 21 for each fungicide level tested. B. Inhibition ratios calculated using acetone amended PDA as control (Ratio_A) for each time point, Days 7, 14 and 21 for each fungicide level tested. The error-bars are the 95% confidence intervals for each mean.</p

Appendix A. A table listing the 12 caves investigated together with descriptive characteristics and treatments received.

A table listing the 12 caves investigated together with descriptive characteristics and treatments received

List of <i>Phyllosticta citricarpa</i> isolates obtained from <i>Citrus sinensis</i> fruits in Florida and their characteristic included in this study.

<p>List of <i>Phyllosticta citricarpa</i> isolates obtained from <i>Citrus sinensis</i> fruits in Florida and their characteristic included in this study.</p

Maximum-likelihood (ML) tree of influenza A NA subtypes.

<p>A: N1; B: N2; C: N5; D: N8. The annotation for each lineage was labeled on the trees. Three lineages in N1 (1A, 1B and 1C), two lineages in N2 (2A and 2B), two lineages in N5 (5A and 5B), and two lineages in N8 (8A and 8B) were classified. The bootstrap values supporting the corresponding lineages are shown to the left of the major nodes. Scale bars indicate the numbers of nucleotide substitutions per site.</p