Tipping the balance for control in host–pathogen interactions; generalized schematic.

<p>To become established in plants or animals, fungal pathogens attempt to disrupt host cell homeostasis while avoiding and/or suppressing host recognition. The host has sophisticated surveillance systems that are poised to rapidly recognize non-self and counter disruptive attempts by pathogens. Signals activated by these surveillance systems can initiate a myriad of host defenses, including the release of reactive oxygen species and hydrolytic enzymes, which thwart the activities of fungal pathogens. The activation of host defense mechanisms also often culminates in the programmed death of host cells or tissue, which limits pathogen spread or dissemination. If attempts by the pathogen to co-opt, subvert, or avoid these host recognition and signaling mechanisms succeed, then the pathogen “wins” the battle for control of the interaction, and disease ensues. If the host wins this battle, then disease is averted.</p

Cell death outcomes following fungal pathogen attack.

<p>Depending on the genotypes of both the plant host and the fungal invader, any one of several cell death pathways can be activated. Although these pathways intersect in cell death, they culminate in disparate outcomes, immunity, or disease as detailed in the text. During the recognition of fungal challenge by the plant, host-controlled HR-PCD leads to a restricted cell death phenotype and ultimately immunity. Conversely, pathogen-mediated PCD suppresses this host recognition, leading to unrestricted spread of the pathogen accompanied by PCD, susceptibility, and disease.</p

Inhibition of autophagy restores A2 pathogenicity.

<p>(A,B) Agar plugs containing actively growing cultures of the OA deficient A2 mutant were inoculated onto leaves of <i>Arabidopsis</i> Col-0 and select <i>Arabidopsis</i> autophagy mutant plants. These mutants showed enhanced susceptibility to the normally non-pathogenic A2 strain. Lesion diameter was monitored over time and all images were recorded 48 hours post inoculation. (C) Tomato leaves were either pre-infiltrated with water (control) or autophagy inhibitors Wortmannin, LY294002, Chloroquine (CQ), and 3-methyladenine (3-MA). Agar plugs containing actively growing A2 were placed on the infiltrated leaves to initiate infection. (B) 48 hours post inoculation; Trypan blue was used to determine the extent of fungal colonization and cleared with acetic acid and ethanol (1: 3, v/v). Images were taken 48 hours post inoculation.</p

Oxidant accumulation in wild type and <i>atg</i> mutant plants.

<p>(A) NBT treated Arabidopsis (Col-0 and two independent <i>atg8a</i> mutant lines) following agar plug inoculation with the A2 mutant. Images were collected 48 hours post inoculation. Dotted lines represent the edge of the observable legion. (B) RT-PCR was used to evaluate the transcript levels of three catalases (CAT1, 2, and 3) and three superoxide dismutases in Col-0 plants following inoculation with wild type <i>S. sclerotiorum</i> (black bars) and the A2 mutant strain (grey bars). *>2 fold change, **>5 fold change.</p

Microscopic examination of cross sections of tomato leaves at the leading edge of the lesion following fungal inoculation.

<p><i>S. sclerotiorum</i> A2 (A) and wild type (B) strains were inoculated onto tomato leaves using colonized agar plugs. 24 hours post inoculation; leaves were post-fixed in osmium tetroxide, and embedded in Spurr's epoxy resin. A microtome was used to cut 400 nm sections. Toluidine blue stain was used to reveal fungal hyphae. H = hyphae. The dotted line represents the leading edge of the visible lesion. Images were collected using an Olympus DP 70 camera and processed with Olympus DP Controller software, version 2.2.1.227.</p

Expression of <i>ced-9</i> in <i>Arabidopsis</i> inhibits wild type infection but does not affect the A2 phenotype.

<p>Agar plugs containing actively growing cultures of wild type <i>S. sclerotiorum</i> (strain 1980) and the OA deficient A2 mutant were inoculated onto Col-0 and <i>ced-9</i> expressing <i>Arabidopsis</i> leaves. (A) Wild type inoculations onto Col-0 plants resulted in typical lesions for this pathogen including a rapid, spreading cell death; however, infection was completely suppressed in <i>ced-9</i> expressing plants. The expression of this gene had no effect on the A2 phenotype. (B) Trypan blue staining indicates the extent of cell death for each genotype/strain combination, including an agar plug control. All images were recorded 48 hours post inoculation.</p

<i>S. sclerotiorum</i> A2 strain induces autophagic structures in plants.

<p><i>S. sclerotiorum</i> wild type and A2 strains were inoculated onto tomato leaves using colonized agar plugs. 24 hours post inoculation; leaves were stained with 100 µM final concentration of MDC (Sigma) in PBS for 30 min. Fluorescence was visualized using an Olympus IX81 inverted fluorescence confocal microscope (Olympus systems, Germany), with an excitation wavelength of 335 nm and an emission wavelength of 508 nm. Images were collected using an Olympus DP 70 camera and processed with Olympus DP Controller software, version 2.2.1.227. Scale bar = 10 µm.</p

Oxalic acid is multifunctional.

<p>OA is a pathogenicity determinant in <i>Sclerotinia</i> that has a number of functions that facilitate fungal pathogenicity. OA inhibits plant defense responses (eg callose deposition) and modulates the host redox environment by blocking the host oxidative burst and creating reducing environment. OA also suppresses autophagy. At later stages, OA accumulation lowers the pH, activates cell wall degrading enzymes and a MAP kinase required for pathogenic sclerotial development. This process culminates in OA induced ROS leading to elicitation of apoptotic cell death and disease. (For further details see Dickman, 2007; Williams et al., 2011).</p

Transmission Electron Microscopy (TEM) fungal inoculated tomato leaves.

<p>Representative TEM images from four independent experiments. (A, G); Healthy non-inoculated leaf tissue. (B–F) Tomato leaves inoculated with the OA deficient A2 strain. (H,I) Tomato leaves inoculated with wild type <i>S. sclerotiorum</i>. Arrows, autolysosomal/autophagosomal-like structures; C, chloroplast; V, vacuole; N, nucleus; Circle, active dismantlement of chloroplast; Rectangle, chromatin condensation within the nucleus. Black scale bars = 2 µm, white scale bars = 1 µm. Sections were examined with a Phillips Morgagni 268 transmission electron microscope at an accelerating voltage of 80 kV. Digital images were recorded with a MegaViewIII digital camera operated with iTEM software.</p

Table_1_Cytoprotective Co-chaperone BcBAG1 Is a Component for Fungal Development, Virulence, and Unfolded Protein Response (UPR) of Botrytis cinerea.DOCX

The Bcl-2 associated athanogene (BAG) family is an evolutionarily conserved group of co-chaperones that confers stress protection against a variety of cellular insults extending from yeasts, plants to humans. Little is known, however, regarding the biological role of BAG proteins in phytopathogenic fungi. Here, we identified the unique BAG gene (BcBAG1) from the necrotrophic fungal pathogen, Botrytis cinerea. BcBAG1 is the homolog of Arabidopsis thaliana AtBAG4, and ectopic expression of BcBAG1 in atbag4 knock-out mutants restores salt tolerance. BcBAG1 deletion mutants (ΔBcbag1) exhibited decreased conidiation, enhanced melanin accumulation and lost the ability to develop sclerotia. Also, BcBAG1 disruption blocked fungal conidial germination and successful penetration, leading to a reduced virulence in host plants. BcBAG1 contains BAG (BD) domain at C-terminus and ubiquitin-like (UBL) domain at N-terminus. Complementation assays indicated that BD can largely restored pathogenicity of ΔBcbag1. Abiotic stress assays showed ΔBcbag1 was more sensitive than the wild-type strain to NaCl, calcofluor white, SDS, tunicamycin, dithiothreitol (DTT), heat and cold stress, suggesting BcBAG1 plays a cytoprotective role during salt stress, cell wall stress, and ER stress. BcBAG1 negatively regulated the expression of BcBIP1, BcIRE1 and the splicing of BcHAC1 mRNA, which are core regulators of unfolded protein response (UPR) during ER stress. Moreover, BcBAG1 interacted with HSP70-type chaperones, BcBIP1 and BcSKS2. In summary, this work demonstrates that BcBAG1 is pleiotropic and not only essential for fungal development, hyphal melanization, and virulence, but also required for response to multiple abiotic stresses and UPR pathway of B. cinerea.</p