Number of ileal bacteria in healthy and diseased dogs.

Number of ileal bacteria in healthy and diseased dogs.</p

Spatial distribution of the number of ileal bacteria based on FISH.

Spatial distribution of the number of ileal bacteria based on FISH.</p

Spatial distribution of the number of colonic bacteria based on FISH.

<p>Spatial distribution of the number of colonic bacteria based on FISH.</p

Probes used for fluorescence in situ hybridization.

<p>Probes used for in situ bacterial identification.</p

Dog cohort demographics.

<p>Canine cohort demographics with regards to age, gender, breed, and diet at the time of endoscopic biopsy.</p

FISH of canine endoscopic biopsies.

<p>Triple color FISH identifies bacterial organisms within different mucosal compartments of endoscopic ileal and colonic biopsies obtained from healthy dogs. Panel A = colon biopsy hybridized with Cy3-Ebac1790; Panel B = colon biopsy hybridized with Cy3-Bac303; Panel C = ileum hybridized with Cy3-Ebac1790; and Panel D = ileum hybridized with Cy3-Ec (<i>E</i>. <i>coli</i>). All other bacteria that hybridize exclusively with the universal probe (Eub338-FITC) appear green. DAPI-stained colonic mucosa with goblet cells appears blue. FM = free mucus; AM = adherent mucus.</p

FISH of canine endoscopic biopsies.

<p>Triple color FISH identifies bacterial organisms present in biofilms adherent to the colonic epithelia in dogs with inflammatory bowel disease (A), granulomatous colitis (B), and colorectal cancer (C). <i>Bacteroides</i> (Bac303-Cy3 probe–panel A), <i>E</i>. <i>coli</i> (Ec-Cy3 probe–panel B), and <i>Eubacterium rectale</i> (Erec482-Cy3 –panel C) populations appear orange against green and blue backgrounds. All other bacteria that hybridize exclusively with the universal probe (Eub338-FITC) appear green. DAPI-stained colonic mucosa with goblet cells appears blue.</p

Number of colonic bacteria in healthy and diseased dogs.

<p>Number of colonic bacteria in healthy and diseased dogs.</p

Clinical characteristics of dogs studied.

<p>CIBDAI = canine IBD activity index with score reported at diagnosis.</p

Triple color FISH identifies mucosal bacteria in the adherent mucus compartment of dogs with IBD following prednisone and dietary therapy.

Panels A-C = ileal tissues and panels D-F = colonic tissues. Panel A = probe Cy3-Faecali698; Panel B = probe Cy3-Ebac; Panel C = probe Cy3- Strc493; Panel D = probe Cy3-Bif164; Panel E = Cy3-Ebac; Panel F = probe FITC-Eub338. All other bacteria that hybridize exclusively with the universal probe (Eub338-FITC) appear green. DAPI-stained intestinal mucosa with goblet cells appears blue. All images at 600x magnification.</p