Comparison of caudal vein and retro orbital injections.

<p> After classical busulfan pre-conditioning (2x25 mg/Kg), female mice received 1000 non manipulated CD34+ cells by i.v. injection either in the caudal vein or retro orbital sinus. Their bone marrow were analyzed 8 weeks later for <b>A.</b> total huCD45 chimerism, <b>B.</b> huCFU content. Each mouse represented by (●), Median represented by (─).</p

Impact of gender on human cells engraftment.

<p>After classical busulfan pre-conditioning (2x25 mg/Kg), female and male mice received 100, 500 or 1000 non manipulated CD34+ cells by i.v. injection in the retro orbital sinus. Their bone marrow were analyzed 8 weeks later for <b>A.</b> total huCD45 chimerism, <b>B.</b> huCFU content. Each mouse represented by ● (female) or ○ (male), Median represented by (─).</p

Impact on engraftment level of time delay between mice pre-conditioning and cell injections.

<p>Female mice received 2 doses of busulfan (25mg/Kg each) at 24 hours, 4 days or 7 days intervals. Mice received 1000 non manipulated CD34+ cells by caudal injection and their bone marrow were analyzed 8 weeks later for <b>A.</b> total huCD45 chimerism, <b>B.</b> B lymphoid (▪) versus myeloid (□) cells ration in CD45+ cells, and <b>C.</b> huCFUs content per femur. Each mouse represented by (●), Median represented by (─).</p