Supplementary results from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Additional Analysis not included in the main text</p

Supplemental Figure legends from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Legends of supplemental figure 1, 2, 3</p

Supplemental Figure 1 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Patient Baseline Parameters and their correlation with Clinical Response measurements</p

Supplemental Figure 2 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Response Predictive Ability of secreted IL-12/IL-10 ratio</p

Supplemental file 1 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Excel Spreadsheet listing all the genes belonging to the 8 modules</p

Supplemental Figure 3 from Expression of CD14, IL10, and Tolerogenic Signature in Dendritic Cells Inversely Correlate with Clinical and Immunologic Response to TARP Vaccination in Prostate Cancer Patients

Interactive 3D plot showing the correlation of module 2 expression with CD14, IL-10, MDC and MCP-1.Each dot represents one DC sample. X, y and z coordinates represent concentration levels of IL-10, MDC, and MCP-1, respectively, size of dots is proportional with % of CD14+ cells, color represents module 2 expression level (red-yellow-white gradient, with red being the lowest expression level and white the highest)</p

Global gene expression and multidimensional scaling analysis of FePro labeled BMSCs.

<p>BMSC samples from 3 donors (FePro-labeled, gold nanoparticle-labeled and unlabeled control) and control cells (3 samples from human embryonic stem cells and 3 samples of adult cells) were analyzed by an oligonucleotide microarray. The multidimensional scaling plot similarly grouped the hES cells together, the adult cells other than BMSCs together in another group, and all the BMSC samples into a third group. The BMSCs did not cluster according to the type of labeling method. hES- human embryonic stem cell; adult indicated the adult cells: Fb-fibroblasts, EC endothelial cells, SMC-smooth muscle cells; BMSC-FePro: bone marrow stromal cellslabeled with FePro; BMSC-Gold: bone marrow stromal cells labeled with gold nanoparticle; BMSC-control: unlabeled BMSC control; D1: donor 1; D2-donor 2; D3 donor 3.</p

Immunohistochemical staining of ossicles derived from FePro or GFP labeled and unlabeled BMSCs.

<p>A representative ossicle derived from unlabeled (A) and FePro labeled (B) BMSCs at 8 weeks, stained with H & E showing comparable abundant bone formation and abundant hematopoiesis. Immunohistochemistry staining for GFP of a representative ossicle derived from BMSCs labeled with both FePro and lentivirus carrying GFP (C) and control unlabeled BMSCs (D).</p

Dilution of FePro in cultured FePro-labeled and unlabeled BMSCs.

<p>The microphotographs show PB staining of BMSCs cultured <i>in vitro</i> at passages 3, 4, 5 and 6 after BMSCs at passage 2 were labeled with FePro.</p

Prussian blue staining of ossicles derived from FePro or unlabeled BMSCs.

<p>Prussian blue (PB) staining of a representative ossicle derived from BMSCs labeled with FePro (A) and control unlabeled BMSCs (B). PB staining of a representative ossicle derived from BMSCs labeled with FePro showing PB⁺ adipocytes (C). PB staining of a representative ossicle derived from labeled BMSCs showing PB⁺ pericytes (D).</p