Aromatic molecules subclasses.

Aromatic molecules subclasses.</p

1-AMA titration curve spectra.

Emission spectra of the pOBP excited at 295 nm in the absence and in the presence of different amounts of 1-AMA.</p

Penta-chlorobenzene competitive assay.

Figure shows the competitive assay. The competitive molecule used was penta-chlorobenzene; fluorescence signal at 481 nm was unaffected.</p

Benzene effect on pOBP structure.

Near-UV CD spectra of pOBP collected at different concentrations of benzene (0–10 μM).</p

Air pollutant molecules that bind to pOBP.

Air pollutant molecules that bind to pOBP.</p

Benzene titration curve fitting.

Plot of the intensity decrease of 1-AMA fluorescence emission at 481 nm as a function of benzene concentration. In red color it is shown the fitting curve obtained by a non-linear function.</p

1-AMA titration curve fitting.

Plot of the intensity increase of 1-AMA fluorescence intensity as a function of fluorophore concentrations.</p

Fluorescence emission spectra at increasing concentrations of benzene.

Figure shows the emission fluorescence spectra of pOBP in the presence of saturating concentrations of 1-AMA. The addition of increasing amounts of benzene determines a decrease of the peak at 481 nm and an increase of the peak at 340 nm.</p

Circular dichroism and fluorescence measurements.

<p>Panel A: Near-UV spectra of MalE2 at pH 4.0 (red), pH 7.0 (green) and pH 10.0 (blue); Panel B: steady state fluorescence spectra of MalE2 at pH 4.0 (red), pH 7.0 (green) and pH 10.0 (blue); inset: the ANS fluorescence spectra of MalE2 at pH 4.0 (red), pH 7.0 (green) and pH 10.0 (blue); Panel C and D: lifetime distribution profiles obtained from dynamics fluorescence measurements at pH 4.0 (red), pH 7.0 (green) and pH 10.0 (blue).</p

Reverse docking results.

The graphical output provided by IdTarget.</p