32 research outputs found

    Hierarchical representation of the 32 clusters generated by Splinecluster

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    <p><b>Copyright information:</b></p><p>Taken from "Time-course analysis of genome-wide gene expression data from hormone-responsive human breast cancer cells"</p><p>http://www.biomedcentral.com/1471-2105/9/S2/S12</p><p>BMC Bioinformatics 2008;9(Suppl 2):S12-S12.</p><p>Published online 26 Mar 2008</p><p>PMCID:PMC2323661.</p><p></p

    Comparison analysis of Diseases and Bio Functions enriched in BEAS-2B cells expressing constitutively active PIK3CA, AKT1 or interfered for PTEN.

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    <p>IPA heatmap displays the top three Disease and Biofunctions enriched by DEGs that are common among BEAS-AKT1-E17K, BEAS-PIK3CA-E545K and BEAS-shPTEN cells (commons, rightmost column) or that are exclusive for each cell line (AKT1, PIK3CA, shPTEN). Diseases and Bio Functions were sorted for p-value, from the most significant (violet) to the least significant (white) values.</p

    Validation of common DEGs in BEAS-C and derivatives cells treated with pharmacological inhibitors of the PI3K/AKT pathway.

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    <p><b>A)</b> Analysis of 7 common DEGs enriched in BioFunctions in BEAS-C and derivatives. Bars are mean±SD of 3 independent experiments performed in triplicate. Statistical analysis was performed by One-way ANOVA test and Dunnett's test for each gene. p-values were indicated in the figure. <b>B)</b> BEAS-C cells and derivatives were treated for 24 hours with the indicated doses of LY294002. Graphs show mRNA levels of the indicated genes in BEAS-C cells and derivatives. Bars are mean±SD of 3 independent experiments conducted in triplicate. Statistical analysis was performed by <i>t-</i>test. p-values as indicated in the figure.</p

    IHC and FISH analysis of AKT2 in NSCLCs.

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    <p>A, left: SCC negative for AKT2 expression; right: SCC positive for AKT2 expression. B, left: ADC negative for AKT2 expression; right: ADC positive for AKT2 expression. Magnification 10Ă— and 40Ă—, respectively. C. Dual-colour fluorescence in situ hybridization analysis of AKT2 gene copy number. FISH analysis of AKT2 (red signals) and chromosome region 19p13.1 (green signals). Left, NSCLC sample with diploid cells; right, NSCLC sample with multiple clustered spots of red signals of AKT2 with 2 chromosome region 19p13.1 signals (gene amplification). Original magnification 100Ă—.</p

    Validation of array results by quantitative RT-PCR analysis.

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    <p><b>(A)</b> Analysis of 10 representative DEGs in BEAS-C and derivatives. Bars are mean ± SD of 4 independent experiments performed in triplicate. Statistical analysis was performed by One-way ANOVA test and Dunnett's test for each gene, p-values as indicated.</p
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