6 research outputs found

    HHV-8 load in isolated PBMCs, B cells and non-B cells from cKS patients and healthy HHV-8-seropositive (HSP) controls according to their peripheral blood B cells count.

    No full text
    a<p>cKS patients were classified according to our classification that takes into account the prevalent type of lesions, localization, clinical behaviour, evolutive pattern and presence of complications.</p>b<p>gEq =  genome Equivalents.</p

    B cells and their non-memory subsets are increased in patients with cKS.

    No full text
    <p>The number of total B cells and CD27<sup>−</sup> B cells was significantly higher in cKS patients (grey bars) than HCs (white bars). All the subsets composing the preimmune/natural effector compartment, namely transitional, pre-naïve, naïve and MZ-like B lymphocytes, were increased in cKS patients, while the subsets composing the antigen-experienced T cell-dependent compartment, namely IgM-only, switched and CD27<sup>−</sup> memory B cells, were unaffected. Data shown as mean ± SE. <i>P</i>-values calculated using the Student <i>t</i> test for independent samples. *<i>P</i><.05; **<i>P</i><.01; ***<i>P</i><.001.</p

    Clinical characteristics of cKS patients.

    No full text
    a<p>Mean ± standard error.</p>b<p>cKS patients were classified according to our classification that takes into account the prevalent type of lesions, localization, clinical behaviour, evolutive pattern and presence of complications <sup>(53,54)</sup>.</p><p>A indicates slow evolution; B, rapid evolution; rapid denotes an increase in the total number of nodules/plaques or in the total area of plaques in three months following the last examination.</p

    B cells from cKS patients show a low state of activation.

    No full text
    <p>B cells from cKS patients (grey bars) expressed lower levels of the costimulatory molecules CD80 and CD86 and higher levels of CD20 than B cells from HCs (white bars). The expression of the indicated markers on total B cells and their CD27<sup>−</sup> and CD27<sup>+</sup> subsets is shown. Data presented as mean ± SE of mean fluorescence intensity (MFI) values. <i>P</i>-values calculated using the Student <i>t</i> test for independent samples. *<i>P</i><.05; **<i>P</i><.01; ***<i>P</i><.001.</p

    B cells from cKS patients show increased resistance to spontaneous apoptosis and unaffected <i>in vivo</i> turnover.

    No full text
    <p>Apoptotic cells were detected by annexin V binding after 24 h culture in unstimulated conditions. <i>A</i>, representative flow cytometric analysis showing annexin V binding gated on total B cells, CD27<sup>−</sup>, MZ-like (CD27<sup>+</sup>IgD<sup>lo</sup>) and switched memory (CD27<sup>+</sup>IgD<sup>−</sup>) B cells, as indicated; comparison between one HC (upper row) and one cKS patient (lower row). B, B cells from cKS patients (grey bars) showed significantly lower annexinV binding than B cells from HCs (white bars); this lower annexin V binding was evident on CD27<sup>−</sup> and MZ-like (CD27<sup>+</sup>IgD<sup>lo</sup>) B cells, roughly composing the preimmune/natural effector compartment, but not on antigen-experienced switched memory (CD27<sup>+</sup>IgD<sup>−</sup>) B cells. Data shown as mean ± SE. <i>C, In vivo</i> cell turnover was estimated by Ki67 staining of freshly isolated PBMCs. Representative flow cytofluorimetric analysis showing Ki67 binding gated on total B cells, CD27<sup>−</sup> and CD27<sup>+</sup> B cells, as indicated; comparison between one HC (upper row) and one cKS patient (lower row). <i>D</i>, The proportion of Ki67<sup>+</sup> cells within total B cells, CD27<sup>−</sup> and CD27<sup>+</sup> B cells did not differ between cKS patients (grey bars) and HCs (white bars). Data shown as mean ± SE. <i>P</i>-values calculated using the Student <i>t</i> test for independent samples.*<i>P</i><.05; **<i>P</i><.01.</p
    corecore