Demographic and clinical characteristics of patients against SARS-CoV-2 culture isolation vs subgenomic RNA concordance.

Demographic and clinical characteristics of patients against SARS-CoV-2 culture isolation vs subgenomic RNA concordance.</p

Primers and probes used to quantify SARS-CoV-2 load and subgenomic RNA.

Primers and probes used to quantify SARS-CoV-2 load and subgenomic RNA.</p

Supplementary information reporting data related to Figs 1, 2 and S2 Fig.

Supplementary information reporting data related to Figs 1, 2 and S2 Fig.</p

Quantasoft panel for RNAse P housekeeping gene of the 51 nasopharyngeal swabs (one well per patient).

In each panel, the first and second wells represent the negative and positive reaction control, respectively. (PDF)</p

Selection criteria for the 51 nasopharyngeal swabs included in the study.

Selection criteria for the 51 nasopharyngeal swabs included in the study.</p

Supplementary information reporting the full protocol used for the detection and quantification of SARS-CoV-2 subgenomic RNAs and viral load.

Supplementary information reporting the full protocol used for the detection and quantification of SARS-CoV-2 subgenomic RNAs and viral load.</p

SARS-CoV-2 subgenomic and genomic load against concordance between SARS-CoV-2 culture isolation and subgenomic RNA detection.

The dot plots represent the quantification of (A) E subgenomic, (B) N subgenomic, (C) SARS-CoV-2 RNA load. Negative concordant is defined as negative culture isolation and negative sgRNA detection. Positive concordant is defined as positive culture isolation and positive sgRNA detection. Discordant is defined as negative culture isolation and positive sgRNA detection. SgRNA was considered positive (+) when at least one subgenomic RNA (N or E) was detectable. The dotted line represents the best cut-off, calculated by ROC curve analysis, to predict a positive culture isolation. *One-sided p-values comparing patients with concordant +/+ results and patients with discordant -/+ results were calculated by the Mann–Whitney test.</p

SARS-CoV-2 genomic and subgenomic RNA load against culture isolation.

SARS-CoV-2 genomic and subgenomic RNA load against culture isolation.</p

Demographic and clinical characteristics of the study population.

Demographic and clinical characteristics of the study population.</p

SARS-CoV-2 subgenomic and genomic load against culture results.

The dot plots represent the quantification of (A) E subgenomic, (B) N subgenomic, and (C) SARS-CoV-2 RNA load in positive and negative cultures. The dotted line represents the best cut-off, calculated by ROC analysis, to predict a positive culture isolation. The bars indicate the median and interquartile range values (IQR).</p