1 research outputs found
Alcohol Dehydrogenase-Catalyzed Gold Nanoparticle Seed-Mediated Growth Allows Reliable Detection of Disease Biomarkers with the Naked Eye
Here,
we reported a strategy-based plasmonic enzyme-linked immunosorbent
assay (ELISA) using alcohol dehydrogenase-catalyzed gold nanoparticle
seed-mediated growth to serve as a colorimetric signal generation
method for detecting disease biomarkers with the naked eye. This system
possesses the advantages of outstanding robustness, sensitivity, and
universality. By using this strategy, we investigated the hepatitis
B surface antigen (HBsAg) and α-fetoprotein (AFP) with the lowest
concentration of naked-eye detection down to 1.0 × 10<sup>–12</sup> g mL<sup>–1</sup>. Experiments with real serum samples from
HBsAg-infected patients are presented, demonstrating the potential
for clinical analysis. Our method eliminates the need for sophisticated
instruments and high detection expenses, making it possible to be
a reliable alternative in resource-constrained regions