5 research outputs found

    ICAM-1 was involved with TNF-α stimulated h-GF binding of SAOS-2.

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    <p>FACS analysis for ICAM-1 expression in h-GF with or without TNF-α (1.16nM) pre-treatment is shown (A), as well as at increasing times over 24 hr of TNF-α (1.16nM) treatment (B), compared with graphs showing the time-course of increased SAOS-2 binding by TNF-α (1.16nM) treated h-GF (C), and a histogram of the effect of blocking antibody against ICAM-1 (D). (A) h-GF expressed ICAM-1 and this was increased by TNF-α with maximal expression by 6 hr of cytokine treatment (B). (C) In an experiment performed at the same time as that shown in (5B), and using h-GF from the same donor, Maximal ICAM-1 expression correlated with maximal binding of SAOS-2 (p<0.001). As seen in the insert (C) examining the first 5 hr of cytokine stimulation in a separate time course experiment, increased SAOS-2 binding occurred between 0.5 and 1 hr of TNF-α (1.16nM) h-GF stimulation, and was maximal by 1.5 hrs (p<0.001). (D) Blocking antibody against ICAM-1 reduced binding of SAOS-2 to both untreated and TNF-α (1.16nM) stimulated h-GF (p<0.04).</p

    TNF-α increased ICAM-1 expression in h-GF and HUVEC, but only increased VCAM-1 in HUVEC.

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    <p>Fluorescence distribution profiles are shown proportionate to the peak incidence of fluorescence in each cell population studied. Background levels of ICAM-1 in both h-GF and HUVEC were appreciably increased following 24 hrs of stimulation with TNF-α (1.16nM). Although a similar effect of the cytokine was seen in HUVEC with regard to VCAM-1 expression, negligible VCAM-1 in h-GF was not significantly increased by TNF-α (1.16nM) stimulation.</p

    SAOS-2 binding to culture plastic and h-GF.

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    <p>SAOS-2 appeared as dark round cells in photomicrographs (arrow heads), as opposed to the elongated paler h-GF (arrows). h-GF increased SAOS-2 binding compared with culture plastic alone (p<0.001), and this was increased by TNF-α (1.16nM) treatment of the h-GF (p<0.001). (Bars = 50 µm).</p

    Decaying effect of TNF-α stimulation on h-GF binding of SAOS-2.

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    <p>A graph is shown of SAOS-2 binding to h-GF, in which h-GF were first stimulated with TNF-α (1.16nM) for 24 hrs, and then washed before further culture for from 0 to 24 hrs in M199 with BSA (4%), either with or without fresh TNF-α (1.16nM). There was decreased SAOS-2 attachment between 6 hr and 12 hrs post-cytokine stimulation (p<0.005), and this reduced to a plateau by 18 hrs (p<0.001).</p