Influences of Relative Humidity and Dwell Time on Silica/Graphene Adhesion Force of a Cone–Plane Contact

Graphene has exceptional electronic, mechanical, and thermal properties, and it is expected to have important applications in integrated circuits and other microelectronic fields. Its performances are greatly affected by surface adhesion force when it is used in a humid environment. In this paper, based on the law of variable water contact angle changing in the process of water vapor condensation, we established a cone–plane contact model, which is related to relative humidity and dwell time, to reveal the internal mechanism of the influence of relative humidity and dwell time on silica/graphene adhesion force. First, the silica/graphene adhesion force dependence of dwell time was measured by atomic force microscopy (AFM) at 45–85% RH. Then, the changing process of the meniscus between the AFM tip and the graphene surface was discussed, and the function of adhesion force with variables of dwell time and contact angle was established. Furthermore, the theoretical and experimental results were compared and analyzed. The results show that with the increase of relative humidity and dwell time, the capillary condensation increases, but the water contact angle of the cone material decreases. This causes the adhesion force to increase first and then decrease after it reaches a threshold value. Furthermore, the variable water contact angle of the graphene surface increases, but the adhesion force decreases gradually with the increase of surface water film. The theoretical results are in good agreement with the experimental results

Time Dependence of the Graphene Surface Adhesion Force of the Sphere–Plane Contact at Different Relative Humidities

Graphene has a promising application prospect in integrated circuits and microelectromechanical systems, and sphere–plane contacts are their common contact types. At present, it is difficult to explain the time dependence of the adhesion force of the sphere–plane contact by conventional theory. Therefore, a single rough peak of sphere–plane contact adhesion force model based on variable water contact angle theory and Bradley contact theory was established; the aim is to reveal the changing law of graphene adhesion force. Then, the time dependence of the graphene surface adhesion force at different humidity levels was investigated by using an atomic force microscopy spherical probe. Finally, a quantitative comparative analysis of the theory and experiment was performed. The results show that the theoretical adhesion force was in good agreement with the experimental measurement results. The time dependence of graphene surface adhesion was not obvious within a relative humidity of 45–55%. When the relative humidity was greater than 65%, the graphene surface adhesion first increased and then decreased with dwell time and finally tended to be stable. Because of the increase in relative humidity, the capillary condensation effect increases, and then the adhesion force increases with the development of the meniscus. When the water film was generated on the sample surface, the adhesion force decreased until the meniscus achieved equilibrium

Engineering the Thermostability of Sucrose Synthase by Reshaping the Subunit Interaction Contributes to Efficient UDP-Glucose Production

The restricted availability of UDP-glucose, an essential precursor that targets oligo/polysaccharide and glycoside synthesis, makes its practical application difficult. Sucrose synthase (Susy), which catalyzes one-step UDP-glucose synthesis, is a promising candidate. However, due to poor thermostability of Susy, mesophilic conditions are required for synthesis, which slow down the process, limit productivity, and prevent scaled and efficient UDP-glucose preparation. Here, we obtained an engineered thermostable Susy (mutant M4) from Nitrosospira multiformis through automated prediction and greedy accumulation of beneficial mutations. The mutant improved the T1/2 value at 55 °C by 27-fold, resulting in UDP-glucose synthesis at 37 g/L/h of space-time yield that met industrial biotransformation standards. Furthermore, global interaction between mutant M4 subunits was reconstructed by newly formed interfaces according to molecular dynamics simulations, with residue Trp162 playing an important role in strengthening the interface interaction. This work enabled effective, time-saving UDP-glucose production and paved the way for rational thermostability engineering of oligomeric enzymes