Structural Dynamics of Lipid Bilayer Membranes Explored by Magnetic Field Effect Based Fluorescence Microscopy

Lipid bilayer membranes are known to exist as heterogeneous and dynamic structures where the molecules are always moving and fluctuating under physiological conditions. Magnetic field effects (MFEs) studied herein are phenomena in which the exciplex emission from an electron donor–acceptor dyad increases or decreases by applying an external magnetic field. The characteristic dependence of MFEs on the viscosity and polarity of the surrounding medium has been applied to investigate the local environments around the probe molecule. In this study, a novel MFE-based fluorescence microscopy technique was developed to explore the structural dynamics of lipid bilayer membranes. The vesicle formation during the membrane deformation was selectively visualized through the MFEs, thus allowing the extraction of information on the cellular dynamics at high temporal and spatial resolutions. This highly versatile and powerful technique is applicable to a wide range of areas, such as biology and material science

Diagnostic indices of E6/E7 mRNA analyses for detecting CIN2+.

Diagnostic indices of E6/E7 mRNA analyses for detecting CIN2+.</p

Diagnostic indices of E6/E7 mRNA analyses for detecting invasive cervical cancer.

Diagnostic indices of E6/E7 mRNA analyses for detecting invasive cervical cancer.</p

E6/E7 mRNA analyses and HPV genotyping in LBC samples from patients with cervical neoplastic diseases.

<p>E6/E7 mRNA analyses and HPV genotyping in LBC samples from patients with cervical neoplastic diseases.</p

Study design.

<p>HPV: human papillomavirus, CIN: cervical intraepithelial neoplasia, ICC: invasive cervical cancer.</p

Diagnostic indices of E6/E7 mRNA analyses for detecting CIN3+.

Diagnostic indices of E6/E7 mRNA analyses for detecting CIN3+.</p

Comparison between DNA and RNA of HPV E6/E7 and human β2-microglobulin (B2M) genes.

<p>The size of E6/E7 DNA is 652bp for HeLa and 622bp for CaSki, same as E6 mRNA. The size of B2M DNA is 775bp and B2M RNA is 148bp.</p

Kaplan-Meier curves for upgraded Pap-test results in followed-up patients with CIN1-2.

<p><i>A</i>, cases positive for both E6 and E7 mRNAs (n = 3) <i>vs</i>. the remainder (n = 28); <i>B</i>, cases with positive E7 mRNAs (n = 4) <i>vs</i>. negative E7 mRNAs (n = 27); <i>C</i>, cases with positive E6 mRNA (n = 15) <i>vs</i>. negative E6 mRNA (n = 16); <i>D</i>, cases with positive HR-HPV DNA (n = 26) <i>vs</i>. negative HR-HPV DNA (n = 5); <i>E</i>, cases with positive HPV16/18 DNA (n = 8) vs. negative HPV16/18 DNA (n = 23).</p

Detection of E6/E7 mRNAs from patients.

<p>E6 transcript is detected in samples 1 and 3, and 2 kinds of E7 transcript are detected in samples 2 and 3.</p

E6/E7 mRNA expression patterns by RT-PCR in human cervical cancer cell lines.

<p>H: full-length E6/E7 (E6), M: spliced E6*I/E7 (E7), L: spliced E6*II/E7 (E7).</p