YIELD POTENTIAL OF DIRECT SEEDED RICE (ORYZA SATIVA L.) AS INFLUENCED BY DIFFERENT SEEDING TECHNIQUE AND WEED MANAGEMENT PRACTICES

A field experiment was conducted during kharif season 2007-08 at Crop Research Center of SVPUAT, Meerut and Uttar Pradesh to find out the suitable crop establishment method and weed management practices in rice. Effective tiller, (297.98), grain/panicle (143.84), grain yield (3711 kg/ha), straw yield (5808 kg/ha) and harvest index (38.98 %) produced higher under broadcast in puddled condition. Among weed management practices, higher number of productive tillers/m2, panicle length (28.79 cm), grains/panicle (148.3), test weight (22.48 g) and grain yield under butachlor @1.5 kg a.i. as pre-emergence followed by almix @ 4 g a.i./ha as post-emergence. Straw yield (5989 kg/ha) was significantly higher under butachlor @1.5 kg a.i. as pre-emergence followed by one hand weeding at 20-25 DAS (days after sowing)

Monocrotophos Induced Apoptosis in PC12 Cells: Role of Xenobiotic Metabolizing Cytochrome P450s

Monocrotophos (MCP) is a widely used organophosphate (OP) pesticide. We studied apoptotic changes and their correlation with expression of selected cytochrome P450s (CYPs) in PC12 cells exposed to MCP. A significant induction in reactive oxygen species (ROS) and decrease in glutathione (GSH) levels were observed in cells exposed to MCP. Following the exposure of PC12 cells to MCP (10−5 M), the levels of protein and mRNA expressions of caspase-3/9, Bax, Bcl2, P53, P21, GSTP1-1 were significantly upregulated, whereas the levels of Bclw, Mcl1 were downregulated. A significant induction in the expression of CYP1A1/1A2, 2B1/2B2, 2E1 was also observed in PC12 cells exposed to MCP (10−5 M), whereas induction of CYPs was insignificant in cells exposed to 10−6 M concentration of MCP. We believe that this is the first report showing altered expressions of selected CYPs in MCP-induced apoptosis in PC12 cells. These apoptotic changes were mitochondria mediated and regulated by caspase cascade. Our data confirm the involvement of specific CYPs in MCP-induced apoptosis in PC12 cells and also identifies possible cellular and molecular mechanisms of organophosphate pesticide-induced apoptosis in neuronal cells

Rice crop residue as fertiliser substitute for enhancing yield and soil health: Identifying the optimum level from multilocation trials in India

To address agricultural challenges like residue burning and excessive inorganic fertilizer use, a two-year (2023–2024) multi-location field experiment was conducted across five diverse Indian agro-ecological zones. Six integrated nutrient management strategies were tested: T1 (100% Recommended Dose of Fertilizers (RDF)), T2 (50% RDF + 50% RDF via residue), T3 (50% RDF + 50% RDF via residue + Pusa decomposer (PD)), T4 (50% RDF via residue + 50% RDF via green manure/green leaf manure), T5 (75% RDF + 25% via residue + PD), and T6 (control). Conventional fertilization (T1) consistently achieved the highest mean grain yield across locations. However, T5 (25% RDF substitution via crop residue) produced statistically comparable yields to T1, even surpassing it by 7.6% at Moncompu. Treatments T2 and T3 generally incurred yield penalties (10–28%), except at Karaikal, where T2 increased yield (+5% in 2023; +11.6% in 2024), due to favorable decomposition conditions. Despite yield trade-offs, T2 and T3 (50% RDF substitution via crop residue) exhibited superior yield stability across locations. Soil nutrient dynamics varied considerably by location and treatment. While T2 and T3 caused partial phosphorus (P) depletion, they consistently improved soil potassium (K) and organic carbon (OC). P buildup was seen in T2/T5 at Karaikal and T1-T5 at Pusa. Similarly, K buildup was seen across most treatments and sites, though K mining was widespread at Pantnagar. Nitrogen (N) and OC showed mixed results, with some treatments leading to accumulation and others depletion. The T6 consistently showed the highest nutrient depletion across all parameters. T1 yielded the highest partial factor productivity of nitrogen (PFP-N) at 47 kg grain/kg N, closely followed by T5 (44 kg grain/kg N). T3 (42.5 kg grain/kg N) was statistically similar to T5, establishing a PFP-N hierarchy of T1 > T5 ≈ T3 > T2 ≈ T4. Grain yield correlated positively with agronomic traits like tiller number, panicle density, and 1000-grain weight, all linked to N and K availability. Thus, co-application of 75% RDF via fertilizers and 25% using crop residues with microbial decomposers (T5) is recommended as a sustainable alternative integrated approach to conventional fertilization

The Anti-Inflammatory and Antibacterial Basis of Human Omental Defense: Selective Expression of Cytokines and Antimicrobial Peptides

BACKGROUND: The wound healing properties of the human omentum are well known and have extensively been exploited clinically. However, the underlying mechanisms of these effects are not well understood. We hypothesize that the omentum tissue promotes wound healing via modulation of anti-inflammatory pathways, and because the omentum is rich in adipocytes, the adipocytes may modulate the anti-inflammatory response. Factors released by human omentum may affect healing, inflammation and immune defense. METHODOLOGY: Six human omentum tissues (non obese, free from malignancy, and any other systemic disorder) were obtained during diagnostic laparoscopies having a negative outcome. Healthy oral mucosa (obtained from routine oral biopsies) was used as control. Cultured adipocytes derived from human omentum were exposed to lipopolysaccharide (LPS) (1-50 ng/mL) for 12-72 hours to identify the non-cytotoxic doses. Levels of expression (mRNA and protein) were carried out for genes associated with pro- and anti-inflammatory cytokine responses and antibacterial/antimicrobial activity using qRT-PCR, western blotting, and cell-based ELISA assays. RESULTS: The study shows significant higher levels of expression (mRNA and protein) of several specific cytokines, and antibacterial peptides in the omentum tissues when compared to oral sub-mucosal tissues. In the validation studies, primary cultures of adipocytes, derived from human omentum were exposed to LPS (5 and 10 ng/mL) for 24 and 48 h. The altered expressions were more pronounced in cultured adipocytes cells when exposed to LPS as compared to the omentum tissue. CONCLUSIONS/SIGNIFICANCE: Perhaps, this is the first report that provides evidence of expressional changes in pro- and anti-inflammatory cytokines and antibacterial peptides in the normal human omentum tissue as well as adipocytes cultured from this tissue. The study provides new insights on the molecular and cellular mechanisms of healing and defense by the omentum, and suggests the potential applicability of cultured adipocytes derived from the omentum for future therapeutic applications

Ameliorative Effects of Dimetylthiourea and N-Acetylcysteine on Nanoparticles Induced Cyto-Genotoxicity in Human Lung Cancer Cells-A549

We study the ameliorative potential of dimetylthiourea (DMTU), an OH• radical trapper and N-acetylcysteine (NAC), a glutathione precursor/H2O2 scavenger against titanium dioxide nanoparticles (TiO2-NPs) and multi-walled carbon nanotubes (MWCNTs) induced cyto-genotoxicity in cultured human lung cancer cells-A549. Cytogenotoxicity was induced by exposing the cells to selected concentrations (10 and 50 µg/ml) of either of TiO2-NPs or MWCNTs for 24 h. Anti-cytogenotoxicity effects of DMTU and NAC were studied in two groups, i.e., treatment of 30 minutes prior to toxic insult (short term exposure), while the other group received DMTU and NAC treatment during nanoparticles exposure, i.e., 24 h (long term exposure). Investigations were carried out for cell viability, generation of reactive oxygen species (ROS), micronuclei (MN), and expression of markers of oxidative stress (HSP27, CYP2E1), genotoxicity (P53) and CYP2E1 dependent n- nitrosodimethylamine-demethylase (NDMA-d) activity. In general, the treatment of both DMTU and NAC was found to be effective significantly against TiO2-NPs and MWCNTs induced cytogenotoxicity in A549 cells. Long-term treatment of DMTU and NAC during toxic insults has shown better prevention than short-term pretreatment. Although, cells responded significantly to both DMTU and NAC, but responses were chemical specific. In part, TiO2-NPs induced toxic responses were mediated through OH• radicals generation and reduction in the antioxidant defense system. While in the case of MWCNTs, adverse effects were primarily due to altering/hampering the enzymatic antioxidant system. Data indicate the applicability of human lung cancer cells-A549 as a pre-screening tool to identify the target specific prophylactic and therapeutic potential of drugs candidate molecules against nanoparticles induced cellular damages

STUDY OF VARIATIONS OF CERVICAL SEGMENT OF INTERNAL CAROTID ARTERY

Introduction: Presence of variations in the course of the cervical (extracranial) part of the internal carotid artery (I.C.A.) in the form of tortuosity, kinking, coiling or looping is a rare condition. These may be attributed to embryological or acquired factors. Patients with such variations may be asymptomatic in some cases, whereas, they may develop cerebrovascular symptoms due to carotid stenosis affecting cerebral circulation. Materials and methods: The present study was performed during routine undergraduate dissection classes in total 21 human cadavers (6 females and 15 males) on bilateral sides. Results: Bilateral kinking and looping of the cervical part of the I.C.A. was found in two cadavers. Conclusions: The risk of transient ischemic attacks (TIA) in patients with carotid stenosis is high and surgical correction is indicated as a part of treatment. Further, patients having these variations are more prone to injury during radical neck dissection and other surgical operations of the neck region. Knowledge about anatomy of such variations provides a framework to review the embryogenesis of the structure and also provides insight into surgical, medical and radiological implications.</jats:p

Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats

Bisphenol A (BPA) is leached out from plastic infant feeding bottles that are filled with warm milk/water due to high temperatures, exposing the infants to BPA. The present study aims to understand the effects of ingestion of BPA leached from plastic infant feeding bottle and delineate the underlying mechanisms in rats. In this study, adult rats of Wistar strain were divided into 3 groups. In the first group, the rats consumed normal food and tap water ad libitum. In the second group, the rats ingested BPA (20 &micro;g/kg bodyweight/day, orally). In the third group, the rats drank water leached from plastic infant feeding bottles. After 30days, tests involving biochemical parameters, histopathological examination, and oxidative stress enzyme markers were performed, and the levels of BPA in plastic-leached water were estimated by HPLC analysis. There were significant biochemical changes in the form of increased alkaline phosphatase (ALP), creatine kinase-muscle/brain (CK-MB), and lactate dehydrogenase (LDH) levels in both treated groups as compared to control group, accompanied by structural damage to the vital organs, and lipid peroxidation, glutathione reductase, and catalase activity were also high in the treated groups. Further, the BPA concentration in plastic leached water was estimated to be 0.1 &plusmn; 0.02 &micro;g/mL