12 research outputs found
Clustering results of patterns obtained by PFGE analysis of 228 <i>Legionella</i> strains.
<p>These strains were isolated from three hot spring recreational areas in Beijing, China during the one-year study period.</p
Relationship between the monthly average number of <i>Legionella</i> and monthly average number of passenger flow.
<p>The curve represents the monthly average number of <i>Legionella</i> (CFU/L), and the histogram represents the monthly average number of passenger flow.</p
The number of spa water samples, spa pools and months contaminated by different <i>Legionella</i> species and serogroups.
<p>The number of spa water samples, spa pools and months contaminated by different <i>Legionella</i> species and serogroups.</p
Clustering results of patterns obtained by PFGE analysis of 55 <i>Legionella</i> strains.
<p>These strains were isolated from one sample of hot spring recreational area A, which was collected in April.</p
Association of physical, chemical, and microbiological water characteristics with <i>Legionella</i> spp. concentration of spa water samples.
<p>Association of physical, chemical, and microbiological water characteristics with <i>Legionella</i> spp. concentration of spa water samples.</p
Additional file 1: of Characterization of Mycobacterium tuberculosis isolates from Hebei, China: genotypes and drug susceptibility phenotypes
Primer information of the MIRU-VNTR loci in this study. (DOCX 15 kb
Intracellular growth of <i>Legionella</i> isolates within J774 cells.
<p>The isolated <i>Legionella</i> strains were grown in BYE broth until they reached early stationary phase. Approximately 2×10<sup>9</sup> bacteria diluted (1∶1,000) in RPMI 1640 tissue culture medium were added to J774 cells (2×10<sup>5</sup> per well) with a multiplicity of infection (MOI) of approximately 10. The infected cells were incubated at 37 °C under 5% CO<sub>2</sub>-air for 1.5 h and washed three times with PBS to remove extracellular bacteria. The CFUs were determined by plating dilutions on BCYE agar plates. The <i>L. pneumophila</i> philadelphila-1 strain and its <i>dotA</i> mutant were used as positive and negative controls, respectively.</p
Minimum spanning tree analysis of 57 <i>Legionella</i> isolates from hot springs in Beijing, China.
<p>In the minimum spanning tree, the STs are displayed as circles. The size of each circle indicates the number of isolates within this particular type, and the STs and the number of isolates in each ST are shown in the circles. The colors of the halo surrounding the STs denote types that belong to the same clonal group.</p
Distribution of <i>Legionella</i> species and serogroups from springs in Beijing, China.
<p>Distribution of <i>Legionella</i> species and serogroups from springs in Beijing, China.</p
Distribution of clonal groups from 35 SBT profiles for <i>L. pneumophila</i> isolates (n = 57) in hot springs in Beijing, China.
<p>Distribution of clonal groups from 35 SBT profiles for <i>L. pneumophila</i> isolates (n = 57) in hot springs in Beijing, China.</p