12 research outputs found

    Intracellular growth of <i>Legionella</i> isolates within J774 cells.

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    <p>The isolated <i>Legionella</i> strains were grown in BYE broth until they reached early stationary phase. Approximately 2×10<sup>9</sup> bacteria diluted (1∶1,000) in RPMI 1640 tissue culture medium were added to J774 cells (2×10<sup>5</sup> per well) with a multiplicity of infection (MOI) of approximately 10. The infected cells were incubated at 37 °C under 5% CO<sub>2</sub>-air for 1.5 h and washed three times with PBS to remove extracellular bacteria. The CFUs were determined by plating dilutions on BCYE agar plates. The <i>L. pneumophila</i> philadelphila-1 strain and its <i>dotA</i> mutant were used as positive and negative controls, respectively.</p

    Minimum spanning tree analysis of 57 <i>Legionella</i> isolates from hot springs in Beijing, China.

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    <p>In the minimum spanning tree, the STs are displayed as circles. The size of each circle indicates the number of isolates within this particular type, and the STs and the number of isolates in each ST are shown in the circles. The colors of the halo surrounding the STs denote types that belong to the same clonal group.</p
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