Additional file 6: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S5. Forest plot and meta-analysis of postoperative newly increased chronic kidney disease (CKD)(stage≥3). (PDF 82 kb

Additional file 11: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S10. Forest plot and subgroup meta-analysis of postoperative long-term change of Cr level. (PDF 83 kb

Additional file 4: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S3. Forest plot and meta-analysis of postoperative long-term eGFR. (PDF 160 kb

Additional file 1: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Table S1. Risk of bias in included studies using modified Newcastle-Ottawa Scale. (DOCX 51 kb

Additional file 5: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S4. Forest plot and meta-analysis of postoperative acute renal failure. (PDF 159 kb

Additional file 10: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S9. Forest plot and subgroup meta-analysis of postoperative long-term % eGFR change. (PDF 84 kb

Additional file 7: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S6. Forest plot and subgroup meta-analysis of postoperative short-term eGFR change. (PDF 84 kb

Additional file 9: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S8. Forest plot and subgroup meta-analysis of postoperative long-term eGFR change. (PDF 84 kb

Additional file 3: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S2. Forest plot and meta-analysis of postoperative short-term Cr level. (PDF 83 kb

Mass spectrometry data and RNA sequencing data of the effect of Gstp1 on SHR-VSMCs

RNA sequencing analysis was performed to recapitulate possible changes in transcriptome profile caused by downregulation of Gstp1 in SHR-VSMCs. Total RNA was extracted from the cells using Trizol reagent. The concentration, quality and integrity were determined using a NanoDrop spectrophotometer (ThermoFisher Scientific, MA, USA). Total RNA required for single library construction was more than 1 μg. Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). Library sequencing was performed on an Illumina Novaseq 6000 platform (Illumina, San Diego, CA, USA).Mass spectrometry analysis was performed to identify Gstp1 binding proteins in SHR-VSMCs. Proteins were immunoprecipitated using anti-Gstp1 antibody and incubated with Protein A/G PLUS-agarose beads for 4 h. Elution buffer (0.1M glycine–HCl) was used to elute antigen from antibody and 1M Tris–HCl was used for neutralization. After trypsin digestion, peptides were desalted using C18 StageTip(Thermo Fisher Scientific, OH, USA), and vacuum dried. The samples were analyzed by LC-MS/MS using an Easy nLC 1200 coupled to a QExactive HF mass spectrometer (Thermo Scientific, OH, USA). Sequence database searching and protein quantification raw mass spectrometry data were analyzed using the MaxQuant software suite (version 1.6.1.0).</p