88 research outputs found

    Understanding the Role of Language Style of Government Response in E-participation: Evidence from a Citizen Inquiry Forum in China

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    In response to the “Internet + Public Service” reformation, Chinese government organizations increasingly use e-participation platforms to interact with citizens, making how government respond to citizens\u27 inquiry increasingly critical in citizen participation. This study is one of the few attempts to study the effect of language style of government response on citizen participation. Based on the language expectancy theory, we hypothesize that government response using appropriate formal and emotional language will change citizen e-participation behavior. Using text data from 11,194 users in a Chinese citizen inquiry forum, we found that emotional language has the positive impact on citizens\u27 continuous participation significantly. And both emotional language and formal language can promote citizens’ participation quality. Moreover, we identified the complementary effects of two languages on e-participation. This study has implications for both researchers and practitioners

    A Study on the Use of Simulation in Synthesizing Path-Following Control Policies for Autonomous Ground Robots

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    We report results obtained and insights gained while answering the following question: how effective is it to use a simulator to establish path following control policies for an autonomous ground robot? While the quality of the simulator conditions the answer to this question, we found that for the simulation platform used herein, producing four control policies for path planning was straightforward once a digital twin of the controlled robot was available. The control policies established in simulation and subsequently demonstrated in the real world are PID control, MPC, and two neural network (NN) based controllers. Training the two NN controllers via imitation learning was accomplished expeditiously using seven simple maneuvers: follow three circles clockwise, follow the same circles counter-clockwise, and drive straight. A test randomization process that employs random micro-simulations is used to rank the ``goodness'' of the four control policies. The policy ranking noted in simulation correlates well with the ranking observed when the control policies were tested in the real world. The simulation platform used is publicly available and BSD3-released as open source; a public Docker image is available for reproducibility studies. It contains a dynamics engine, a sensor simulator, a ROS2 bridge, and a ROS2 autonomy stack the latter employed both in the simulator and the real world experiments.Comment: 8 pages, 7 figure

    Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis

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    The growth in world population, climate change, and resource scarcity necessitate a sustainable increase in crop productivity. Photosynthesis in major crops is limited by the inefficiency of the key CO2-fixing enzyme Rubisco, owing to its low carboxylation rate and poor ability to discriminate between CO2 and O2. In cyanobacteria and proteobacteria, carboxysomes function as the central CO2-fixing organelles that elevate CO2 levels around encapsulated Rubisco to enhance carboxylation. There is growing interest in engineering carboxysomes into crop chloroplasts as a potential route for improving photosynthesis and crop yields. Here, we generate morphologically correct carboxysomes in tobacco chloroplasts by transforming nine carboxysome genetic components derived from a proteobacterium. The chloroplast-expressed carboxysomes display a structural and functional integrity comparable to native carboxysomes and support autotrophic growth and photosynthesis of the transplastomic plants at elevated CO2. Our study provides proof-of-concept for a route to engineering fully functional CO2-fixing modules and entire CO2-concentrating mechanisms into chloroplasts to improve crop photosynthesis and productivity

    Research on elastic recoil and restoration of vessel pulsatility of Zn-Cu biodegradable coronary stents

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    Coronary stents made of zinc (Zn)-0.8 copper (Cu) (in wt%) alloy were developed as biodegradable metal stents (Zn-Cu stents) in this study. The mechanical properties of the Zn-Cu stents and the possible gain effects were characterized by in vitro and in vivo experiments compared with 316L stainless steel stents (316L stents). Young's modulus of the as-extruded Zn-0.8Cu alloy and properties of the stents, including their intrinsic elastic recoil, stent trackability were evaluated compared with 316L stents. In vivo study was also conducted to evaluate restoration of pulsatility of vessel segment implanted stents. Both Zn-Cu stents and 316L stents have good acute lumen gain. By comparison, the advantages of Zn-Cu stents are as follows: (I) Zn-Cu stents have less intrinsic elastic recoil than 316L stents; (II) stent trackability indicates that Zn-Cu stents have a smaller push force when passing through curved blood vessels, which may cause less mechanical stimulation to blood vessels; (III) in vivo study suggests that Zn-Cu stents implantation better facilitates the recovery of vascular pulsatility.Research funding: This study has financial support from the National Key Research and Development Program of China (No. 2016YFC1102500).Scopu

    Producing fast and active Rubisco in tobacco to enhance photosynthesis

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    Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous plant Rubisco with a faster Rubisco is anticipated to enhance crop photosynthesis and productivity. However, the requirement of chaperones for Rubisco expression and assembly has obstructed the efficient production of functional foreign Rubisco in chloroplasts. Here, we report the engineering of a Form 1A Rubisco from the proteobacterium Halothiobacillus neapolitanus in Escherichia coli and tobacco (Nicotiana tabacum) chloroplasts without any cognate chaperones. The native tobacco gene encoding Rubisco large subunit was genetically replaced with H. neapolitanus Rubisco (HnRubisco) large and small subunit genes. We show that HnRubisco subunits can form functional L8S8 hexadecamers in tobacco chloroplasts at high efficiency, accounting for ∌40% of the wild-type tobacco Rubisco content. The chloroplast-expressed HnRubisco displayed a ∌2-fold greater carboxylation rate and supported a similar autotrophic growth rate of transgenic plants to that of wild type in air supplemented with 1% CO2. This study represents a step towards the engineering of a fast and highly active Rubisco in chloroplasts to improve crop photosynthesis and growth

    Enhancing E-Participation through Citizen-to-Citizen Interactions: Evidence from a Government-sponsored Forum in China

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    Citizen participation is a crucial challenge for government departments, and the potential of citizen-to-citizen (C2C) interactions to enhance government governance remains under-explored. This paper examines the impact of citizens' online social capital in C2C interactions on e-participation behaviour. The findings show that interaction frequency, trust and shared topics in C2C interactions can positively influence citizens' e-participation, with interaction frequency having the most significant impact. This study contributes to the literature on citizen social capital and e-participation and provides guidance for the construction of government e-participation platforms
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