sj-pdf-1-imr-10.1177_03000605221109396 - Supplemental material for Confusing delayed hematemesis, unusual arterial hemorrhage after pancreaticoduodenectomy: a case report

Supplemental material, sj-pdf-1-imr-10.1177_03000605221109396 for Confusing delayed hematemesis, unusual arterial hemorrhage after pancreaticoduodenectomy: a case report by Luna Wang, Gaoli Guo, Jianhua Yu, Ling Lin, Jianhui Yang and Baochun Lu in Journal of International Medical Research</p

Tumor growth in nude mice.

Tumor growth in nude mice.</p

Fangchinoline suppresses the proliferation of GBC cells.

(A) The cell viability curves detected using CCK8 assays after treating GBC-SD and NOZ cells with different fangchinoline concentrations. (B) The colony formation ability of GBC-SD and NOZ cells treated with different fangchinoline concentrations. Data are presented as mean ± SD. *P0.05. **P0.01. ***P0.001, compared with the DMSO group.</p

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Fangchinoline induces the apoptosis of GBC cells.

(A) Hoechst 33258 staining of GBC cells treated with fangchinoline. (B) TUNEL assay of the apoptosis effect of GBC cells with high fangchinoline concentration. (C) Annexin V/ PI apoptosis assay of GBC-SD. Annexin V (+)/PI (−) or Annexin V (−)/PI (+) were considered as apoptotic cells, Annexin V (−)/PI (−) cells were alive, and Annexin V (+)/PI (+) cells were necrotic.</p

Fangchinoline suppresses the oncogenesis of GBC-SD cells in vivo.

(A) The photograph of nude mice and xenograft tumors. (B) The volume and weight of xenograft tumors were measured. **P < 0.01, compared with the DMSO group.</p

Fangchinoline inhibits the PI3K/Akt/XIAP signaling axis.

(A) Results of FAK, p-FAK (Tyr576/577), Src, p-Src (Tyr527), PI3K, p-PI3K (Tyr458/Tyr199), AKT, p-AKT (Ser473), mTOR, p-mTOR (Ser2448), and XIAP relative protein expression using Western blot. (B) GBC cells were incubated with p-Akt activator SC-79 alone or combined with fangchinoline before lysis for Western blot. Data are presented as mean ± SD. *P0.05. **PP<0.001, compared with the DMSO group.</p