Establishment of a cohort of HC of CL patients in <i>L. braziliensis</i> endemic area.

<p>Establishment of a cohort of HC of CL patients in <i>L. braziliensis</i> endemic area.</p

Demographic and epidemiologic features of patients with cutaneous leishmaniasis and household contacts with and without evidence of immune response to leishmania antigen.

<p>Values with identical superscripts are significantly different. Statistical tests and p-values are below.</p><p>P-values for pair-wise Bonferroni post-hoc tests:</p>a<p> = 0.002,</p>b<p> = 0.004,</p>c<p> = 0.005,</p>d<p> = 0.003,</p>e<p> = 0.009,</p>f<p><0.001.</p>*<p>one-way ANOVA test.</p>**<p>Pearson's chi-square test.</p>***<p>before 4:00 pm.</p

Correlation between IFN-γ and CXCL10 production to soluble <i>Leishmania</i> antigen.

<p>The data was obtained from 54 household contacts (HC) with evidence of immune response and 51 household contacts without evidence of immune response which were selected among individuals living in the same households as HC with evidence of immune response. Values of IFN-γ (pg/ml) and CXCL10 (pg/ml) from 105 HC were plotted in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001947#pntd-0001947-g003" target="_blank">Figure 3</a> and these data were analyzed by the Spearman correlation test. P<0.0001, r = 0,74.</p

Production of CXCL9 and CXCL10 in cultures with or without stimulation with soluble <i>Leishmania</i> antigen.

<p>Aliquots of 1 ml of heparinized peripheral blood were cultured in the absence (medium) or stimulated with SLA for 72 hours. Chemokines production was determined by ELISA and results are expressed as pg/ml. Figure A shows the data obtained for CXCL9 and Figure B CXCL10. Values statistically different are indicated as: *** p<0.0001; *p<0.05; Wilcoxon test; GraphPad Prism 4. Medium; SLA.</p

Spontaneous and SLA induced IFN-γ and chemokines production by cells of household contacts of cutaneous leishmaniasis patients with and without evidence of immune response to leishmania antigen.

<p>Values with identical super-scripts are significantly different. Statistical tests and p-values are below:</p><p>P-values for Kruskal Wallis Dunn's post test:</p>a<p><0.05,</p>b<p><0.001,</p>c<p><0.001,</p>d<p><0.001,</p>e<p><0.001,</p>f<p><0.001,</p>g<p><0.001,</p>h<p><0.001,</p>i<p> = <0.001,</p>j<p> = 0.01,</p>k<p><0.001,</p>l<p><0.001,</p>m<p><0.001,</p>n<p><0.001.</p>*<p>one-way ANOVA test;</p>**<p>Kruskal Wallis test;</p>***<p><a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001947#s3" target="_blank">Results</a> expressed as Median value (inter-quartile range).</p