62 research outputs found

    Formation of Nanotunnels Inside a Resist Film in Laser Interference Lithography

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    A few kinds of 2-diazo-1-naphthoquinone-4-sulfonates of poly­(4-hydroxylstyrene) were prepared to form one-component i-line photoresists. In the laser interference lithography experiments of some of the photoresists, nanotunnels were observed to be aligned in the interior of the resist film. The shape and size of the nanotunnels remain virtually unchanged even under an increased exposure dose, indicating that the exposure energy is confined within the tunnel space. The formation of the nanotunnels results from the effect of standing waves and the permeation of developer from the surface deep into the resist films

    Data_Sheet_1_Carbon and nitrogen metabolic regulation in freshwater plant Ottelia alismoides in response to carbon limitation: A metabolite perspective.docx

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    Carbon and nitrogen metabolism are basic, but pivotal metabolic pathways in plants and are tightly coupled. Maintaining the balance of carbon and nitrogen metabolism is critical for plant survival. Comprehensively revealing the metabolic balance of carbon–nitrogen interactions is important and helpful for understanding the adaptation of freshwater plants to CO2 limited aqueous environment. A comprehensive metabolomics analysis combined with physiological measurement was performed in the freshwater plant Ottelia alismoides acclimated to high and low CO2, respectively, for a better understanding of how the carbon and nitrogen metabolic adjustment in freshwater plants respond to carbon limitation. The present results showed that low CO2 acclimated O. alismoides exhibited significant diurnal titratable acidity and malate fluctuations, as well as an opposite diel pattern of starch change and high enzymatic activities required for crassulacean acid metabolism (CAM) photosynthesis, which indicates that CAM was induced under low CO2. Moreover, the metabolomic analysis showed that most intermediates of glycolysis, pentose phosphate pathway (PPP) and tricarboxylic acid (TCA) cycle, were increased under low CO2, indicative of active respiration in low-CO2-treated O. alismoides. Meanwhile, the majority of amino acids involved in pathways of glutamate and arginine metabolism, aspartate metabolism, and the branched-chain amino acids (BCAAs) metabolism were significantly increased under low CO2. Notably, γ-aminobutyric acid (GABA) level was significantly higher in low CO2 conditions, indicating a typical response with GABA shunt compensated for energy deprivation at low CO2. Taken together, we conclude that in low-CO2-stressed O. alismoides, CAM photosynthesis was induced, leading to higher carbon and nitrogen as well as energy requirements. Correspondingly, the respiration was greatly fueled via numerous starch degradation to ensure CO2 fixation in dark, while accompanied by linked promoted N metabolism, presumably to produce energy and alternative carbon sources and nitrogenous substances for supporting the operation of CAM and enhancing tolerance for carbon limitation. This study not only helps to elucidate the regulating interaction between C and N metabolism to adapt to different CO2 but also provides novel insights into the effects of CO2 variation on the metabolic profiling of O. alismoides.</p

    DataSheet_1_Functional identification of purine permeases reveals their roles in caffeine transport in tea plants (Camellia sinensis).xlsx

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    Caffeine is a characteristic secondary metabolite in tea plants. It confers tea beverage with unique flavor and excitation effect on human body. The pathway of caffeine biosynthesis has been generally established, but the mechanism of caffeine transport remains unclear. Here, eight members of purine permeases (PUPs) were identified in tea plants. They had diverse expression patterns in different tissues, suggesting their broad roles in caffeine metabolism. In this study, F1 strains of "Longjing43" ♂ × "Baihaozao" ♀ and different tea cultivars were used as materials to explore the correlation between caffeine content and gene expression. The heterologous expression systems of yeast and Arabidopsis were applied to explore the function of CsPUPs. Correlation analysis showed that the expressions of CsPUP1, CsPUP3.1, and CsPUP10.1 were significantly negatively correlated with caffeine content in tea leaves of eight strains and six cultivars. Furthermore, subcellular localization revealed that the three CsPUPs were not only located in plasma membrane but also widely distributed as circular organelles in cells. Functional complementation assays in yeast showed that the three CsPUPs could partly or completely rescue the defective function of fcy2 mutant in caffeine transport. Among them, transgenic yeast of CsPUP10.1 exhibited the strongest transport capacity for caffeine. Consistent phenotypes and functions were further identified in the CsPUP10.1-over-expression Arabidopsis lines. Taken together, it suggested that CsPUPs were involved in caffeine transport in tea plants. Potential roles of CsPUPs in the intracellular transport of caffeine among different subcellular organelles were proposed. This study provides a theoretical basis for further research on the PUP genes and new insights for caffeine metabolism in tea plants.</p

    Volcano plot displaying differentially expressed miRNAs identified using miRNA-seq in Tibetan and Yorkshire pigs.

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    <p>The y-axis represents the mean expression value of log10 (<i>P</i>-value) and the x-axis displays the log<sub>2</sub>-fold change value. Up-regulated and downregulated miRNAs are shown in red and green, respectively. Black dots indicate genes with no significant change in expression.</p

    Petrogenesis and tectonic setting of Early Cretaceous magmatism in the Jiwa area, central Lhasa Terrane, Tibet

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    <p>New zircon LA-ICP-MS U–Pb ages, Sr-Nd isotopic data, and whole-rock major and trace element data from Early Cretaceous volcanic rocks are reported for the Jiwa area in the southern central Lhasa Terrane of Tibet. These mainly silicic volcanic rocks and subordinate intermediate-basic volcanic rocks have long been considered to be Pliocene (Wuyu Group) or Eocene (Pana Formation) in age. However, our new zircon U–Pb ages constrain the timing of eruption to the Early Cretaceous (124.6 ± 1.6–126.1 ± 1.1 Ma); thus, we have redefined these volcanic rocks as the Lower Cretaceous Zenong Group. The silicic volcanic rocks feature high-K calc-alkaline to shoshonitic compositions and are mostly strongly peraluminous, rich in Rb, Th, and light rare earth elements (LREEs), and depleted in Nb, Ta, P, and Ti. They are also characterized by negative whole-rock <i>ε</i>Nd(t) values (–9.1 to –13.1) and variable <sup>87</sup>Sr/<sup>86</sup>Sr ratios. Thus, the geochemical and zircon U–Pb age data of the Jiwa volcanic rocks suggest that these rocks are associated with a continental arc and are mostly likely derived from anatexis of ancient continental crustal material and minor basalt-derived melts. The discovery of Early Cretaceous volcanic rocks in the southern central Lhasa Terrane extends the duration of magmatism triggered by southward subduction of the Bangong-Nujiang oceanic lithosphere from the Late Jurassic to the Early Cretaceous. The spatial distribution of magmatism is also extended 70–80 km to the south. The Lower Cretaceous volcanic rocks in the Jiwa area are proposed to be a result of bi-directional subduction, with southward subduction of the Bangong-Nujiang oceanic crust and northward subduction of the Yarlung Zangbo oceanic crust. The bi-directional subduction of the oceanic lithosphere and gravitational sinking led to slab retreat at ca. 125 Ma. The roll-back of the slab would have then led to back-arc extension and asthenospheric upwelling. The subduction-induced decompression melting of the mantle led to the generation of widespread rhyolitic volcanism with continental arc geochemical signatures.</p

    Venn diagrams demonstrating relationships among miRNA in Tibetan and Yorkshire pigs.

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    <p>(A) Venn diagram for total miRNAs (contained novel miRNAs and known miRNAs). (B) Venn diagram for known porcine miRNAs. TP and YP miRNAs marked in blue and yellow cycle, respectively. Tibetan pigs (TP) and Yorkshire pigs (YP).</p

    Five cardiac tissue differentially expressed (DE) miRNAs validated by reverse-transcription quantitative polymerase chain reaction.

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    <p>Relative expression levels of DE miRNAs. Upper letters (a, b) on bars denote significantly different expression levels in the same miRNA (<i>P</i> < 0.05).</p

    Image_1_Functional identification of purine permeases reveals their roles in caffeine transport in tea plants (Camellia sinensis).tif

    No full text
    Caffeine is a characteristic secondary metabolite in tea plants. It confers tea beverage with unique flavor and excitation effect on human body. The pathway of caffeine biosynthesis has been generally established, but the mechanism of caffeine transport remains unclear. Here, eight members of purine permeases (PUPs) were identified in tea plants. They had diverse expression patterns in different tissues, suggesting their broad roles in caffeine metabolism. In this study, F1 strains of "Longjing43" ♂ × "Baihaozao" ♀ and different tea cultivars were used as materials to explore the correlation between caffeine content and gene expression. The heterologous expression systems of yeast and Arabidopsis were applied to explore the function of CsPUPs. Correlation analysis showed that the expressions of CsPUP1, CsPUP3.1, and CsPUP10.1 were significantly negatively correlated with caffeine content in tea leaves of eight strains and six cultivars. Furthermore, subcellular localization revealed that the three CsPUPs were not only located in plasma membrane but also widely distributed as circular organelles in cells. Functional complementation assays in yeast showed that the three CsPUPs could partly or completely rescue the defective function of fcy2 mutant in caffeine transport. Among them, transgenic yeast of CsPUP10.1 exhibited the strongest transport capacity for caffeine. Consistent phenotypes and functions were further identified in the CsPUP10.1-over-expression Arabidopsis lines. Taken together, it suggested that CsPUPs were involved in caffeine transport in tea plants. Potential roles of CsPUPs in the intracellular transport of caffeine among different subcellular organelles were proposed. This study provides a theoretical basis for further research on the PUP genes and new insights for caffeine metabolism in tea plants.</p
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