Allele Frequencies of Endothelial Nitric Oxide Synthase Gene Polymorphisms.

a<p>p = 0.001, ^bp<0.001, ^cp = 0.059, ^dp = 0.127, ^ep = 0.037, ^fp = 0.042, ^gp = 0.390, ^hp = 0.465 <i>vs.</i> health control.</p

eNOS gene polymorphism analysis.

<p>A) PCR-RFLP analysis of exon 7 PCR product. B) Allele b/b of eNOS intron 4. C) Allele a/b of eNOS intron 4. D) Allele a/a of eNOS intron 4.</p

Genotype Frequencies of Endothelial Nitric Oxide Synthase Gene Polymorphisms.

a<p>p<0.001, ^bp = 0.0094, ^cp = 0.0203, ^dp = 0.0357 <i>vs.</i> healthy control.</p

Clinical etiological classification of ANFH patients.

<p>DOD: duration of disease.^ap<0.0001,</p>b<p>p<0.0001 <i>vs.</i> idiopathic group.</p

Concise synthesis of pyrrolo[2,3-<i>d</i>]pyrimidine derivatives via the Cu-catalyzed coupling reaction

<p>We reported a green and simple Cu-catalyzed method for the efficient synthesis of 2-chloro-7-cyclopentyl-<i>N,N</i>-dimethyl-7H-pyrrolo[2,3-<i>d</i>]pyrimidine-6-carboxamide as the key intermediate in the synthetic approaches to pyrrolo[2,3-<i>d</i>]pyrimidine derivatives from 5-bromo-2,4-dichloropyrimidine through two routes in four steps and five steps, respectively. This method provided green and economical approaches toward numerous pyrrolo[2,3-<i>d</i>]pyrimidine derivatives.</p

Spectral Broadening in Cr³⁺-Doped Sr_0.92Mg_0.91Al_10.1O₁₇ NIR Phosphor Realized by Multi-crystallographic Site Occupation

Near-infrared phosphor-converted light-emitting diodes (NIR pc-LEDs) play an important role in nondestructive testing, medical imaging, food analysis, and so on. Broadband NIR phosphors are needed in order to cover the characteristic wavelengths of various matters. In this paper, a NIR phosphor, Sr0.92Mg0.91Al10.1O17 (SMAO):Cr3+, with a full width at half maximum (FWHM) of 12–102 nm is achieved as a function of Cr3+ concentration. Two Cr3+ sites are observed by means of excitation spectra and fluorescence decay curves. The spectral broadening with the increase of Cr3+ concentration is attributed to the increase of occupation ratio on the weak crystal field site by Cr3+. In addition, the difference in thermal stability between 2E and 4T2 is employed to conduct the temperature measurement with a relative sensitivity of 0.43% K–1 at 270 K. A NIR pc-LED is fabricated using SMAO:0.03Cr3+ phosphor combined with a 395 nm LED chip with radiation flux of 2.2 mW@10 mA and 15 mW@100 mA

Table1_Complete genome sequence analysis of a novel alkane-degrading bacterial strain, Acinetobacter vivianii KJ-1, and its diesel degradation ability.DOCX

With the increasing demand for diesel in various countries, the ecological pollution caused by the improper use, storage, and accidental leakage of diesel needs to be addressed urgently. As an environmentally friendly and cost-effective method, bioremediation generally uses various microorganisms to remove pollutants from the environment. Here, the strain KJ-1, obtained through an enrichment culture using n-dodecane from oil-contaminated soil near a gas station as the substrate, was identified as Acinetobacter vivianii according to its morphology, biochemistry, and molecular biology. The isolate KJ-1 was able to use diesel as a sole carbon source and more than 40% of diesel was degraded after 12 days of incubation with strain KJ-1 in mineral salts medium. The most suitable diesel concentration and nitrogen source concentrations were 4,140 mg/L and 350–700 mg/L, respectively, for diesel degradation and bacterial growth. The optimal initial pH and temperature for strain KJ-1 growth and diesel degradation were 6.5–8.0 and 20–37°C, respectively. To investigate the diesel-degrading mechanisms of this strain, the complete genome was sequenced and annotated. The complete genome consists of one chromosome with a total length of 3,927,757 base pairs and a G + C content of 41.5%. The genes related to the two-component regulatory system and alkane degradation were analyzed. In addition, two putative alkane monooxygenases were analyzed, and the protein sequences were characterized and compared with other AlkBs in Acinetobacter spp. using sequences downloaded from NCBI. The results demonstrated that A. vivianii KJ-1 may be particularly useful for future bioremediation of diesel-polluted soil.</p

DataSheet1_Biomechanical Effects of 3D-Printed Bioceramic Scaffolds With Porous Gradient Structures on the Regeneration of Alveolar Bone Defect: A Comprehensive Study.DOCX

In the repair of alveolar bone defect, the microstructure of bone graft scaffolds is pivotal for their biological and biomechanical properties. However, it is currently controversial whether gradient structures perform better in biology and biomechanics than homogeneous structures when considering microstructural design. In this research, bioactive ceramic scaffolds with different porous gradient structures were designed and fabricated by 3D printing technology. Compression test, finite element analysis (FEA) revealed statistically significant differences in the biomechanical properties of three types of scaffolds. The mechanical properties of scaffolds approached the natural cancellous bone, and scaffolds with pore size decreased from the center to the perimeter (GII) had superior mechanical properties among the three groups. While in the simulation of Computational Fluid Dynamics (CFD), scaffolds with pore size increased from the center to the perimeter (GI) possessed the best permeability and largest flow velocity. Scaffolds were cultured in vitro with rBMSC or implanted in vivo for 4 or 8 weeks. Porous ceramics showed excellent biocompatibility. Results of in vivo were analysed by using micro-CT, concentric rings and VG staining. The GI was superior to the other groups with respect to osteogenicity. The Un (uniformed pore size) was slightly inferior to the GII. The concentric rings analysis demonstrated that the new bone in the GI was distributed in the periphery of defect area, whereas the GII was distributed in the center region. This study offers basic strategies and concepts for future design and development of scaffolds for the clinical restoration of alveolar bone defect.</p

Additional file 1 of Efficient bone regeneration of BMP9-stimulated human periodontal ligament stem cells (hPDLSCs) in decellularized bone matrix (DBM) constructs to model maxillofacial intrabony defect repair

Additional file 1. Fig. S1: The effect of BMP9 on the osteogenic differentiation ability of hDMSCs in vitro. (A) BMP9 stimulated the expression of early responsive target genes in hDMSCs. Human DPSCs, SCAPs and PDLSCs were infected with Ad-GFP and Ad-BMP9 for 24h. The expression levels of ID1, ID2 and CTGF in different groups were evaluated by qPCR. (B) BMP9 stimulated the expression of osteogenic genes in hDMSCs. The expression levels of RUNX2, OPN, ALP and OSX in different groups were evaluated by qPCR on days 3 and 7. The assays were carried out in three independent experiments. All data are the means ± SDs; *P<0.05 and **P<0.01. Fig. S2: Reconstructed 3D micro-CT images showing the heights of new bone formation. Representative images are shown. Fig. S3: The heights of new bone were analyzed using Mimics Research 19.0 software. All values are the means ± SDs; *P < 0.05, **P < 0.01, ***P < 0.001. Fig. S4: Reconstructed 3D micro-CT images of the whole constructs. The yellow regions represent the plain DBM constructs, while the blue regions are indicative of newly formed bony masses. Representative images are shown. Fig. S5: Immunohistochemical staining of osteogenic marker in newly formed tissues. (A) Representative immunohistochemical staining images of osteocalcin (OCN). Scale bar=50 μm. (B) The OCN positive cells were quantified by IOD (Integrated Optical Density) with Image-Pro Plus. All values are the means ± SDs; ***P < 0.001, # no statistical significance. Table S1: Primer sequences