Understanding the multiwavelength observation of Geminga's TeV halo: the role of anisotropic diffusion of particles

In this letter we propose that the X-ray and the TeV observations in the vicinity of Geminga can be understood in the framework of anisotropic diffusion of injected electrons/positrons. This interpretation only requires the turbulence in the vicinity of Geminga to be sub-Alfv\'enic with the local mean magnetic field direction approximately aligned with our line of sight towards Geminga, without invoking extreme conditions for the environment, such as an extremely small diffusion coefficient and a weak magnetic field of strength $<1\mu$G as suggested in previous literature.Comment: 7 pages, 4 figures, including Supplemental Material, PRL accepte

Transfer Function Analysis: Modelling Residential Building Costs in New Zealand by Including the Influences of House Price and Work Volume

An accurate cost estimate not only plays a key role in project feasibility studies but also in achieving a final successful outcome. Conventionally, estimating cost typically relies on the experience of professionals and cost data from previous projects. However, this process is complex and time-consuming, and it is challenging to ensure the accuracy of the estimates. In this study, the bivariate and multivariate transfer function models were adopted to estimate and forecast the building costs of two types of residential buildings in New Zealand: Low-rise buildings and high-rise buildings. The transfer function method takes advantage of the merits of univariate time series analysis and the power of explanatory variables. In the dynamic project conduction environment, simply including building cost data in the cost forecasting models is not valid for making predictions, because the change in demand must be considered. Thus, the time series of house prices and work volume were used to explain exogenous effects in the transfer function model. To demonstrate the effectiveness of transfer function models, this study compared the results generated by the transfer function models with autoregressive integrated moving average models. According to the forecasting performance of the models, the proposed approach achieved better results than autoregressive integrated moving average models. The proposed method can provide accurate cost estimates that can help stakeholders in project budget planning and management strategy making at the early stage of a project

On the minimum jet power of TEV BL Lac objects in the p-γ\gamma model

We study the requirement on the jet power in the conventional p-$\gamma$ models (photopion production and Bethe-Heitler pair production) for TeV BL Lac objects. We select a sample of TeV BL Lac objects whose SEDs are difficult to be explained by the one-zone leptonic model. Based on the relation between the p-$\gamma$ interaction efficiency and the opacity of $\gamma\gamma$ absorption, we find that the detection of TeV emission poses upper limits on the p-$\gamma$ interaction efficiencies in these sources and hence minimum jet powers can be derived accordingly. We find that the obtained minimum jet powers exceed the Eddington luminosity of the supermassive black holes. Implications for the accretion mode of the supermassive black hole in these BL Lac objects and the origin of their TeV emissions are discussed.Comment: 11 pages, 4 figures, 2 tables, accepted for publication in Ap

Cardiac Specific Overexpression of Mitochondrial Omi/HtrA2 Induces Myocardial Apoptosis and Cardiac Dysfunction.

Myocardial apoptosis is a significant problem underlying ischemic heart disease. We previously reported significantly elevated expression of cytoplasmic Omi/HtrA2, triggers cardiomyocytes apoptosis. However, whether increased Omi/HtrA2 within mitochondria itself influences myocardial survival in vivo is unknown. We aim to observe the effects of mitochondria-specific, not cytoplasmic, Omi/HtrA2 on myocardial apoptosis and cardiac function. Transgenic mice overexpressing cardiac-specific mitochondrial Omi/HtrA2 were generated and they had increased myocardial apoptosis, decreased systolic and diastolic function, and decreased left ventricular remodeling. Transiently or stably overexpression of mitochondria Omi/HtrA2 in H9C2 cells enhance apoptosis as evidenced by elevated caspase-3, -9 activity and TUNEL staining, which was completely blocked by Ucf-101, a specific Omi/HtrA2 inhibitor. Mechanistic studies revealed mitochondrial Omi/HtrA2 overexpression degraded the mitochondrial anti-apoptotic protein HAX-1, an effect attenuated by Ucf-101. Additionally, transfected cells overexpressing mitochondrial Omi/HtrA2 were more sensitive to hypoxia and reoxygenation (H/R) induced apoptosis. Cyclosporine A (CsA), a mitochondrial permeability transition inhibitor, blocked translocation of Omi/HtrA2 from mitochondrial to cytoplasm, and protected transfected cells incompletely against H/R-induced caspase-3 activation. We report in vitro and in vivo overexpression of mitochondrial Omi/HtrA2 induces cardiac apoptosis and dysfunction. Thus, strategies to directly inhibit Omi/HtrA2 or its cytosolic translocation from mitochondria may protect against heart injury

Adiponectin improves coronary no-reflow injury by protecting the endothelium in rats with type 2 diabetes mellitus.

To determine the effect of adiponectin (APN) on the coronary no-reflow (NR) injury in rats with Type 2 diabetes mellitus (T2DM), 80 male Sprague-Dawley rats were fed with a high-sugar-high-fat diet to build a T2DM model. Rats received vehicle or APN in the last week and then were subjected to myocardial ischemia reperfusion (MI/R) injury. Endothelium-dependent vasorelaxation of the thoracic aorta was significantly decreased and serum levels of endothelin-1 (ET-1), intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were noticably increased in T2DM rats compared with rats without T2DM. Serum APN was positively correlated with the endothelium-dependent vasorelaxation, but negatively correlated with the serum level of ET-1. Treatment with APN improved T2DM-induced endothelium-dependent vasorelaxation, recovered cardiac function, and decreased both NR size and the levels of ET-1, ICAM-1 and VCAM-1. Hypoadiponectinemia was associated with the aggravation of coronary NR in T2DM rats. APN could alleviate coronary NR injury in T2DM rats by protecting the endothelium and improving microcirculation

Preparation and biological activity of the monoclonal antibody against the second extracellular loop of the angiotensin II type 1 receptor

The current study was to prepare a mouse-derived antibody against the angiotensin II type 1 receptor (AT1-mAb) based on monoclonal antibody technology, to provide a foundation for research on AT1-AA-positive diseases. Balb/C mice were actively immunized with the second extracellular loop of the angiotensin II type 1 receptor (AT1R-ECII). Then, mouse spleen lymphocytes were fused with myeloma cells and monoclonal hybridomas that secreted AT1-mAb were generated and cultured, after which those in logarithmic-phase were injected into the abdominal cavity of mice to retrieve the ascites. Highly purified AT1-mAb was isolated from mouse ascites after injection with 1 × 107 hybridomas. A greater amount of AT1-mAb was purified from mouse ascites compared to the cell supernatant of hybridomas. AT1-mAb purified from mouse ascites constricted the thoracic aorta of mice and increased the beat frequency of neonatal rat myocardial cells via the AT1R, identical to the effects of AT1-AA extracted from patients’ sera. Murine blood pressure increased after intravenous injection of AT1-mAb via the tail vein. High purity and good biological activity of AT1-mAb can be obtained from mouse ascites after intraperitoneal injection of monoclonal hybridomas that secrete AT1-mAb. These data provide a simple tool for studying AT1-AA-positive diseases