Site-Specific Amphiphilic Magnetic Copolymer Nanoaggregates for Dual Imaging

Molecular imaging along with combinations of imaging modalities can provide a thorough understanding of disease, in particular, tumors. Magnetic resonance imaging (MRI) offers exceptional tissue contrast and resolution; whereas optical imaging provides high sensitivity. Hence a norbornene based copolymer (<b>Nor-Cob-Py-Fol</b>) is reported in this paper as a dual-imaging agent. <b>Nor-Cob-Py-Fol</b> having Co²⁺ complex, pyrene and poly­(ethylene glycol) derived folate, have been synthesized using ring-opening metathesis polymerization (<b>ROMP</b>). All the monomers and polymers are characterized by ¹H NMR, IR, GPC, and TGA techniques. The morphology of the copolymer nanoaggregates has been evaluated with DLS, TEM, and SEM techniques. The functionalization of Co²⁺ to the polymer is monitored by FTIR, ¹H NMR, and ¹³C NMR spectroscopy. Furthermore, the presence of Co²⁺ in the nanoaggregates is confirmed by the EDX (SEM) technique. To prove the MRI capabilities nature of copolymer nanoaggregates, NMR experiment is performed at room temperature. Cell viability studies suggest the biocompatibility nature of the copolymer. Flow cytometry as well as epifluoroscence microscope experiments clearly demonstrate the dual-imaging ability of the newly designed copolymer. The much higher relaxivity ratio (<i>r</i>₂/<i>r</i>₁) of the present method clearly establishes the superiority of our system as one of the best contrast agents known to the practitioners of magnetic resonance imaging

Site-Specific Amphiphilic Magnetic Copolymer Nanoaggregates for Dual Imaging

Molecular imaging along with combinations of imaging modalities can provide a thorough understanding of disease, in particular, tumors. Magnetic resonance imaging (MRI) offers exceptional tissue contrast and resolution; whereas optical imaging provides high sensitivity. Hence a norbornene based copolymer (<b>Nor-Cob-Py-Fol</b>) is reported in this paper as a dual-imaging agent. <b>Nor-Cob-Py-Fol</b> having Co²⁺ complex, pyrene and poly­(ethylene glycol) derived folate, have been synthesized using ring-opening metathesis polymerization (<b>ROMP</b>). All the monomers and polymers are characterized by ¹H NMR, IR, GPC, and TGA techniques. The morphology of the copolymer nanoaggregates has been evaluated with DLS, TEM, and SEM techniques. The functionalization of Co²⁺ to the polymer is monitored by FTIR, ¹H NMR, and ¹³C NMR spectroscopy. Furthermore, the presence of Co²⁺ in the nanoaggregates is confirmed by the EDX (SEM) technique. To prove the MRI capabilities nature of copolymer nanoaggregates, NMR experiment is performed at room temperature. Cell viability studies suggest the biocompatibility nature of the copolymer. Flow cytometry as well as epifluoroscence microscope experiments clearly demonstrate the dual-imaging ability of the newly designed copolymer. The much higher relaxivity ratio (<i>r</i>₂/<i>r</i>₁) of the present method clearly establishes the superiority of our system as one of the best contrast agents known to the practitioners of magnetic resonance imaging

Site-Specific Amphiphilic Magnetic Copolymer Nanoaggregates for Dual Imaging

Molecular imaging along with combinations of imaging modalities can provide a thorough understanding of disease, in particular, tumors. Magnetic resonance imaging (MRI) offers exceptional tissue contrast and resolution; whereas optical imaging provides high sensitivity. Hence a norbornene based copolymer (<b>Nor-Cob-Py-Fol</b>) is reported in this paper as a dual-imaging agent. <b>Nor-Cob-Py-Fol</b> having Co²⁺ complex, pyrene and poly­(ethylene glycol) derived folate, have been synthesized using ring-opening metathesis polymerization (<b>ROMP</b>). All the monomers and polymers are characterized by ¹H NMR, IR, GPC, and TGA techniques. The morphology of the copolymer nanoaggregates has been evaluated with DLS, TEM, and SEM techniques. The functionalization of Co²⁺ to the polymer is monitored by FTIR, ¹H NMR, and ¹³C NMR spectroscopy. Furthermore, the presence of Co²⁺ in the nanoaggregates is confirmed by the EDX (SEM) technique. To prove the MRI capabilities nature of copolymer nanoaggregates, NMR experiment is performed at room temperature. Cell viability studies suggest the biocompatibility nature of the copolymer. Flow cytometry as well as epifluoroscence microscope experiments clearly demonstrate the dual-imaging ability of the newly designed copolymer. The much higher relaxivity ratio (<i>r</i>₂/<i>r</i>₁) of the present method clearly establishes the superiority of our system as one of the best contrast agents known to the practitioners of magnetic resonance imaging

Super paramagnetic Norbornene Copolymer Functionalized with Biotin and Doxorubicin: A Potential Unique Site-Specific Theranostic Agent

Polymer-based nanosystems have been extensively explored either as therapeutic agents or bioimaging probes in the cancer diagnosis. However, very few systems are successful in combining both therapy and imaging. Herein, a new class of norbornene based copolymer, <b>Nor-Dox-Cob-Btn</b> is proposed as potential theranostics agent for tumor diagonosis. The copolymer (<b>Nor-Dox-Cob-Btn</b>) with doxorubicin, cobalt carbonyl complex, and biotin as pendent functionalized group is synthesized, using ring opening metathesis polymerization (ROMP). The cell viability, drug release, NMR relaxation, NMR 1-D image and Epi fluorescence microscopy studies on <b>Nor-Dox-Cob-Btn</b> nanocarrier are thoroughly studied. The effect of nanocarrier on transverse relaxation (<i>T</i>₂) of water molecule and NMR 1-D image suggest that the nanocarrier has the potential application in magnetic resonance imaging agent. The <i>T</i>₂-weighted MRI agent, along with biotin receptor assisted pH responsive doxorubicin release from <b>Nor-Dox-Cob-Btn</b>, prompts us to envision this newly developed polymer for future application in theranostics