Functional annotation of <i>C</i>. <i>idella</i> Unigenes.

(A) Go term distribution for the cellular component, biological process and molecular function categories. (B) eggNOG annotation.</p

DE genes pathways classification of <i>C</i>. <i>idella</i>.

<p>DE genes pathways classification of <i>C</i>. <i>idella</i>.</p

Phagosome-related pathway gene expression in control and experimental group intra-peritoneal injection with 2.7×10⁷ CFU/mL (100 μL/fish) <i>Aeromonas hydrophila</i>, as determined by qRT-PCR.

(A) TUBB, (B) CTSS, (C) MARCO, (D) LAMP1/2. The black and the gray column represent selected gene and sequencing data, respectively. Values are presented as the mean ± SD (n = 6). The expression levels with * indicate significant differences with control group (p < 0.05).</p

Complement and coagulation cascades pathway gene expression in control and experimental group intra-peritoneal injection with 2.7×10⁷ CFU/mL (100 μL/fish) <i>Aeromonas hydrophila</i>, as determined by qRT-PCR.

<p>(A1 and A2) C4, (B1 and B2) C3, (C1 and C2) MBL, (D1 and D2) C2, (E1 and E2) C5, (F1 and F2) C8A, (G) C1QA, (H) C1QC, (I) F2R, (J) FGG, (K) C8B, (L) CFB, (M) CTSZ, and (N) LIPA. The black and the gray column represent selected gene and sequencing data, respectively. Values are presented as the mean ± SD (n = 6). The expression levels with * indicate significant differences with control group (p<0.05).</p

Antigen processing system gene expression in control and experimental group intra-peritoneal injection with 2.7×10⁷ CFU/mL (100 μL/fish) <i>Aeromonas hydrophila</i>, as determined by qRT-PCR.

<p>(A) HTPG, (B) PSME2, (C) PSME1, (D) HSPA4. The black and the gray column represent selected gene and sequencing data, respectively. Values are presented as the mean ± SD (n = 6). The expression levels with * indicate significant differences with control group (p<0.05).</p

Unigene distribution in the <i>C</i>. <i>idella</i>.

<p>(A) E-value distribution of unigenes annotated into public databases with an E-value cut-off of 1E-50. (B) Identity distribution of unigenes annotated into public databases with an E-value cut-off of 1E-50.</p

DE genes related to immune system.

<p>DE genes related to immune system.</p

KEGG pathway in hierarchy 2.

<p>Unigenes were classified in metabolism, genetic information processing, environmental information processing, cellular processes, organismal systems and human diseases categories.</p

Summaries of cDNA libraries sequencing of grass carp by Illumina NextSeq 500 platform.

<p>Summaries of cDNA libraries sequencing of grass carp by Illumina NextSeq 500 platform.</p

Statistics of the annotation results for the <i>C</i>. <i>idella</i> unigenes.

<p>Statistics of the annotation results for the <i>C</i>. <i>idella</i> unigenes.</p